Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09410 and SGL_RS18845 are separated by 121 nucleotides SGL_RS18845 and SGL_RS18850 are separated by 152 nucleotides SGL_RS18850 and SGL_RS09420 overlap by 91 nucleotides SGL_RS09420 and SGL_RS09425 overlap by 8 nucleotides SGL_RS09425 and SGL_RS09430 are separated by 30 nucleotides
SGL_RS09410: SGL_RS09410 - IS3 family transposase, at 1,615,869 to 1,616,528
_RS09410
SGL_RS18845: SGL_RS18845 - hypothetical protein, at 1,616,650 to 1,616,751
_RS18845
SGL_RS18850: SGL_RS18850 - IS630 transposase-related protein, at 1,616,904 to 1,617,466
_RS18850
SGL_RS09420: SGL_RS09420 - DUF86 domain-containing protein, at 1,617,376 to 1,617,813
_RS09420
SGL_RS09425: SGL_RS09425 - nucleotidyltransferase family protein, at 1,617,806 to 1,618,096
_RS09425
SGL_RS09430: SGL_RS09430 - leucyl aminopeptidase, at 1,618,127 to 1,619,710
_RS09430
Position (kb) 1617
1618 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 1617.734 kb on + strand, within SGL_RS09420 at 1617.875 kb on + strand, within SGL_RS09425 at 1617.908 kb on + strand, within SGL_RS09425 at 1617.987 kb on + strand, within SGL_RS09425 at 1617.987 kb on + strand, within SGL_RS09425 at 1618.015 kb on - strand, within SGL_RS09425 at 1618.243 kb on - strand at 1618.297 kb on + strand, within SGL_RS09430 at 1618.430 kb on - strand, within SGL_RS09430 at 1618.448 kb on + strand, within SGL_RS09430 at 1618.455 kb on + strand, within SGL_RS09430 at 1618.479 kb on + strand, within SGL_RS09430 at 1618.519 kb on + strand, within SGL_RS09430 at 1618.561 kb on + strand, within SGL_RS09430 at 1618.561 kb on + strand, within SGL_RS09430 at 1618.569 kb on - strand, within SGL_RS09430 at 1618.720 kb on - strand, within SGL_RS09430
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,617,734 + SGL_RS09420 0.82 +0.8 1,617,875 + SGL_RS09425 0.24 -0.0 1,617,908 + SGL_RS09425 0.35 -0.0 1,617,987 + SGL_RS09425 0.62 -0.1 1,617,987 + SGL_RS09425 0.62 +0.2 1,618,015 - SGL_RS09425 0.72 +0.8 1,618,243 - +4.6 1,618,297 + SGL_RS09430 0.11 +4.4 1,618,430 - SGL_RS09430 0.19 +4.9 1,618,448 + SGL_RS09430 0.20 +4.4 1,618,455 + SGL_RS09430 0.21 +4.4 1,618,479 + SGL_RS09430 0.22 +3.6 1,618,519 + SGL_RS09430 0.25 +4.8 1,618,561 + SGL_RS09430 0.27 +4.3 1,618,561 + SGL_RS09430 0.27 +4.6 1,618,569 - SGL_RS09430 0.28 +5.3 1,618,720 - SGL_RS09430 0.37 +4.2
Or see this region's nucleotide sequence