Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09150 and SGL_RS09155 are separated by 308 nucleotides SGL_RS09155 and SGL_RS09160 are separated by 441 nucleotides SGL_RS09160 and SGL_RS09165 are separated by 110 nucleotides SGL_RS09165 and SGL_RS09170 are separated by 236 nucleotides
SGL_RS09150: SGL_RS09150 - acetolactate synthase large subunit, at 1,556,475 to 1,558,118
_RS09150
SGL_RS09155: SGL_RS09155 - riboflavin synthase subunit alpha, at 1,558,427 to 1,558,627
_RS09155
SGL_RS09160: SGL_RS09160 - thioredoxin family protein, at 1,559,069 to 1,559,611
_RS09160
SGL_RS09165: SGL_RS09165 - hypothetical protein, at 1,559,722 to 1,560,150
_RS09165
SGL_RS09170: SGL_RS09170 - class II fructose-bisphosphatase, at 1,560,387 to 1,561,424
_RS09170
Position (kb)
1559
1560 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1558.222 kb on + strand at 1558.266 kb on + strand at 1558.274 kb on - strand at 1558.669 kb on + strand at 1558.937 kb on - strand at 1559.104 kb on + strand at 1559.123 kb on - strand at 1559.170 kb on + strand, within SGL_RS09160 at 1559.393 kb on + strand, within SGL_RS09160 at 1559.776 kb on - strand, within SGL_RS09165 at 1559.907 kb on - strand, within SGL_RS09165 at 1559.947 kb on + strand, within SGL_RS09165 at 1559.955 kb on - strand, within SGL_RS09165 at 1560.019 kb on - strand, within SGL_RS09165 at 1560.050 kb on + strand, within SGL_RS09165 at 1560.058 kb on - strand, within SGL_RS09165 at 1560.123 kb on + strand at 1560.230 kb on - strand at 1560.292 kb on + strand at 1560.293 kb on - strand at 1560.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,558,222 + +0.3 1,558,266 + +0.0 1,558,274 - -0.0 1,558,669 + +0.2 1,558,937 - +0.2 1,559,104 + +0.2 1,559,123 - +0.8 1,559,170 + SGL_RS09160 0.19 +2.3 1,559,393 + SGL_RS09160 0.60 -3.2 1,559,776 - SGL_RS09165 0.13 +0.3 1,559,907 - SGL_RS09165 0.43 +0.8 1,559,947 + SGL_RS09165 0.52 +0.8 1,559,955 - SGL_RS09165 0.54 +0.6 1,560,019 - SGL_RS09165 0.69 -0.3 1,560,050 + SGL_RS09165 0.76 +0.7 1,560,058 - SGL_RS09165 0.78 +2.2 1,560,123 + -0.2 1,560,230 - +0.7 1,560,292 + +0.5 1,560,293 - +0.5 1,560,293 - +0.2
Or see this region's nucleotide sequence