Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08605 and SGL_RS08610 are separated by 21 nucleotides SGL_RS08610 and SGL_RS08615 are separated by 40 nucleotides SGL_RS08615 and SGL_RS08620 are separated by 26 nucleotides
SGL_RS08605: SGL_RS08605 - DUF3120 domain-containing protein, at 1,434,901 to 1,435,623
_RS08605
SGL_RS08610: SGL_RS08610 - adenylate/guanylate cyclase domain-containing protein, at 1,435,645 to 1,436,658
_RS08610
SGL_RS08615: SGL_RS08615 - glutathione peroxidase, at 1,436,699 to 1,437,163
_RS08615
SGL_RS08620: SGL_RS08620 - thiolase family protein, at 1,437,190 to 1,438,602
_RS08620
Position (kb)
1435
1436
1437 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1434.722 kb on + strand at 1434.722 kb on + strand at 1434.745 kb on - strand at 1434.745 kb on - strand at 1434.890 kb on - strand at 1434.971 kb on - strand at 1435.146 kb on + strand, within SGL_RS08605 at 1435.245 kb on - strand, within SGL_RS08605 at 1435.257 kb on + strand, within SGL_RS08605 at 1435.264 kb on + strand, within SGL_RS08605 at 1435.333 kb on - strand, within SGL_RS08605 at 1435.444 kb on - strand, within SGL_RS08605 at 1435.497 kb on - strand, within SGL_RS08605 at 1435.808 kb on + strand, within SGL_RS08610 at 1435.941 kb on + strand, within SGL_RS08610 at 1435.949 kb on - strand, within SGL_RS08610 at 1436.072 kb on - strand, within SGL_RS08610 at 1436.077 kb on + strand, within SGL_RS08610 at 1436.085 kb on - strand, within SGL_RS08610 at 1436.146 kb on - strand, within SGL_RS08610 at 1436.305 kb on + strand, within SGL_RS08610 at 1436.384 kb on + strand, within SGL_RS08610 at 1436.502 kb on - strand, within SGL_RS08610 at 1436.550 kb on + strand, within SGL_RS08610 at 1436.558 kb on - strand at 1436.601 kb on - strand at 1436.619 kb on - strand at 1436.629 kb on - strand at 1436.651 kb on + strand at 1436.732 kb on - strand at 1436.751 kb on - strand, within SGL_RS08615 at 1436.818 kb on - strand, within SGL_RS08615 at 1436.831 kb on + strand, within SGL_RS08615 at 1436.831 kb on - strand, within SGL_RS08615 at 1436.836 kb on + strand, within SGL_RS08615 at 1436.868 kb on + strand, within SGL_RS08615 at 1436.998 kb on - strand, within SGL_RS08615 at 1437.197 kb on + strand at 1437.389 kb on + strand, within SGL_RS08620 at 1437.448 kb on + strand, within SGL_RS08620 at 1437.450 kb on + strand, within SGL_RS08620 at 1437.551 kb on + strand, within SGL_RS08620 at 1437.551 kb on + strand, within SGL_RS08620 at 1437.584 kb on + strand, within SGL_RS08620 at 1437.619 kb on + strand, within SGL_RS08620
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,434,722 + +0.2 1,434,722 + +0.3 1,434,745 - -0.1 1,434,745 - -0.3 1,434,890 - +0.3 1,434,971 - +0.3 1,435,146 + SGL_RS08605 0.34 +1.4 1,435,245 - SGL_RS08605 0.48 +2.2 1,435,257 + SGL_RS08605 0.49 +2.7 1,435,264 + SGL_RS08605 0.50 +3.7 1,435,333 - SGL_RS08605 0.60 +3.3 1,435,444 - SGL_RS08605 0.75 +2.6 1,435,497 - SGL_RS08605 0.82 +2.6 1,435,808 + SGL_RS08610 0.16 +1.5 1,435,941 + SGL_RS08610 0.29 +2.2 1,435,949 - SGL_RS08610 0.30 +1.6 1,436,072 - SGL_RS08610 0.42 +2.1 1,436,077 + SGL_RS08610 0.43 +1.6 1,436,085 - SGL_RS08610 0.43 +1.5 1,436,146 - SGL_RS08610 0.49 +1.8 1,436,305 + SGL_RS08610 0.65 +1.6 1,436,384 + SGL_RS08610 0.73 +0.2 1,436,502 - SGL_RS08610 0.85 +1.9 1,436,550 + SGL_RS08610 0.89 +1.5 1,436,558 - +1.5 1,436,601 - +1.9 1,436,619 - +0.9 1,436,629 - +1.2 1,436,651 + +0.4 1,436,732 - +0.1 1,436,751 - SGL_RS08615 0.11 +0.4 1,436,818 - SGL_RS08615 0.26 +1.1 1,436,831 + SGL_RS08615 0.28 -0.1 1,436,831 - SGL_RS08615 0.28 -0.2 1,436,836 + SGL_RS08615 0.29 -0.2 1,436,868 + SGL_RS08615 0.36 +1.2 1,436,998 - SGL_RS08615 0.64 -0.5 1,437,197 + +0.1 1,437,389 + SGL_RS08620 0.14 +0.1 1,437,448 + SGL_RS08620 0.18 +0.1 1,437,450 + SGL_RS08620 0.18 -0.2 1,437,551 + SGL_RS08620 0.26 +0.5 1,437,551 + SGL_RS08620 0.26 -0.2 1,437,584 + SGL_RS08620 0.28 -0.3 1,437,619 + SGL_RS08620 0.30 +0.0
Or see this region's nucleotide sequence