Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08530 and SGL_RS08535 are separated by 20 nucleotides SGL_RS08535 and SGL_RS08540 are separated by 36 nucleotides SGL_RS08540 and SGL_RS08545 are separated by 313 nucleotides
SGL_RS08530: SGL_RS08530 - type I DNA topoisomerase, at 1,414,126 to 1,416,822
_RS08530
SGL_RS08535: SGL_RS08535 - NIL domain-containing protein, at 1,416,843 to 1,417,247
_RS08535
SGL_RS08540: SGL_RS08540 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 1,417,284 to 1,418,681
_RS08540
SGL_RS08545: SGL_RS08545 - Uma2 family endonuclease, at 1,418,995 to 1,419,573
_RS08545
Position (kb) 1417
1418
1419 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 1416.557 kb on + strand at 1416.802 kb on - strand at 1416.817 kb on + strand at 1417.083 kb on + strand, within SGL_RS08535 at 1417.091 kb on - strand, within SGL_RS08535 at 1417.219 kb on - strand at 1417.450 kb on - strand, within SGL_RS08540 at 1417.452 kb on + strand, within SGL_RS08540 at 1417.572 kb on + strand, within SGL_RS08540 at 1417.692 kb on + strand, within SGL_RS08540 at 1417.823 kb on - strand, within SGL_RS08540 at 1417.860 kb on + strand, within SGL_RS08540 at 1417.943 kb on - strand, within SGL_RS08540 at 1417.989 kb on + strand, within SGL_RS08540 at 1417.994 kb on - strand, within SGL_RS08540 at 1418.466 kb on + strand, within SGL_RS08540 at 1419.297 kb on + strand, within SGL_RS08545
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,416,557 + +3.4 1,416,802 - +1.7 1,416,817 + -0.3 1,417,083 + SGL_RS08535 0.59 +3.4 1,417,091 - SGL_RS08535 0.61 +3.8 1,417,219 - +3.2 1,417,450 - SGL_RS08540 0.12 +0.3 1,417,452 + SGL_RS08540 0.12 +1.1 1,417,572 + SGL_RS08540 0.21 +0.9 1,417,692 + SGL_RS08540 0.29 +1.0 1,417,823 - SGL_RS08540 0.39 +0.6 1,417,860 + SGL_RS08540 0.41 +1.6 1,417,943 - SGL_RS08540 0.47 +0.7 1,417,989 + SGL_RS08540 0.50 +0.7 1,417,994 - SGL_RS08540 0.51 -0.0 1,418,466 + SGL_RS08540 0.85 -0.2 1,419,297 + SGL_RS08545 0.52 -0.6
Or see this region's nucleotide sequence