Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08170 and SGL_RS08175 are separated by 9 nucleotides SGL_RS08175 and SGL_RS08180 are separated by 50 nucleotides SGL_RS08180 and SGL_RS08185 are separated by 97 nucleotides
SGL_RS08170: SGL_RS08170 - H(+)/Cl(-) exchange transporter ClcA, at 1,342,775 to 1,344,130
_RS08170
SGL_RS08175: SGL_RS08175 - serine hydrolase, at 1,344,140 to 1,345,066
_RS08175
SGL_RS08180: SGL_RS08180 - phycobiliprotein lyase, at 1,345,117 to 1,345,677
_RS08180
SGL_RS08185: SGL_RS08185 - hypothetical protein, at 1,345,775 to 1,346,023
_RS08185
Position (kb)
1344
1345
1346 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1343.320 kb on - strand, within SGL_RS08170 at 1343.417 kb on - strand, within SGL_RS08170 at 1343.417 kb on - strand, within SGL_RS08170 at 1343.417 kb on - strand, within SGL_RS08170 at 1343.564 kb on - strand, within SGL_RS08170 at 1343.648 kb on - strand, within SGL_RS08170 at 1343.648 kb on - strand, within SGL_RS08170 at 1343.648 kb on - strand, within SGL_RS08170 at 1343.768 kb on + strand, within SGL_RS08170 at 1343.883 kb on + strand, within SGL_RS08170 at 1343.896 kb on - strand, within SGL_RS08170 at 1343.954 kb on + strand, within SGL_RS08170 at 1343.962 kb on - strand, within SGL_RS08170 at 1344.012 kb on - strand at 1344.037 kb on + strand at 1344.294 kb on + strand, within SGL_RS08175 at 1344.302 kb on - strand, within SGL_RS08175 at 1344.426 kb on + strand, within SGL_RS08175 at 1344.434 kb on - strand, within SGL_RS08175 at 1344.460 kb on + strand, within SGL_RS08175 at 1344.468 kb on - strand, within SGL_RS08175 at 1344.645 kb on + strand, within SGL_RS08175 at 1344.753 kb on + strand, within SGL_RS08175 at 1344.876 kb on - strand, within SGL_RS08175 at 1344.891 kb on + strand, within SGL_RS08175 at 1344.934 kb on + strand, within SGL_RS08175 at 1345.114 kb on + strand at 1345.122 kb on + strand at 1345.536 kb on - strand, within SGL_RS08180 at 1345.689 kb on - strand at 1345.883 kb on - strand, within SGL_RS08185 at 1346.012 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,343,320 - SGL_RS08170 0.40 +0.1 1,343,417 - SGL_RS08170 0.47 +0.2 1,343,417 - SGL_RS08170 0.47 -0.7 1,343,417 - SGL_RS08170 0.47 +0.2 1,343,564 - SGL_RS08170 0.58 -0.2 1,343,648 - SGL_RS08170 0.64 -0.3 1,343,648 - SGL_RS08170 0.64 -1.0 1,343,648 - SGL_RS08170 0.64 +0.8 1,343,768 + SGL_RS08170 0.73 -0.2 1,343,883 + SGL_RS08170 0.82 -1.9 1,343,896 - SGL_RS08170 0.83 +0.0 1,343,954 + SGL_RS08170 0.87 -0.3 1,343,962 - SGL_RS08170 0.88 -0.2 1,344,012 - -0.5 1,344,037 + +0.1 1,344,294 + SGL_RS08175 0.17 -3.5 1,344,302 - SGL_RS08175 0.17 -1.5 1,344,426 + SGL_RS08175 0.31 +0.8 1,344,434 - SGL_RS08175 0.32 -1.5 1,344,460 + SGL_RS08175 0.35 -3.0 1,344,468 - SGL_RS08175 0.35 -2.9 1,344,645 + SGL_RS08175 0.54 -3.3 1,344,753 + SGL_RS08175 0.66 -1.8 1,344,876 - SGL_RS08175 0.79 -1.2 1,344,891 + SGL_RS08175 0.81 -2.0 1,344,934 + SGL_RS08175 0.86 -1.8 1,345,114 + +0.0 1,345,122 + -0.1 1,345,536 - SGL_RS08180 0.75 -2.7 1,345,689 - -0.1 1,345,883 - SGL_RS08185 0.43 +0.5 1,346,012 + -0.1
Or see this region's nucleotide sequence