Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08150 and SGL_RS08155 are separated by 8 nucleotides SGL_RS08155 and SGL_RS08160 are separated by 56 nucleotides SGL_RS08160 and SGL_RS08165 overlap by 11 nucleotides SGL_RS08165 and SGL_RS08170 are separated by 114 nucleotides
SGL_RS08150: SGL_RS08150 - deoxyribodipyrimidine photo-lyase, 8-HDF type, at 1,339,656 to 1,341,122
_RS08150
SGL_RS08155: SGL_RS08155 - Spy/CpxP family protein refolding chaperone, at 1,341,131 to 1,341,637
_RS08155
SGL_RS08160: SGL_RS08160 - hypothetical protein, at 1,341,694 to 1,342,062
_RS08160
SGL_RS08165: SGL_RS08165 - sigma-70 family RNA polymerase sigma factor, at 1,342,052 to 1,342,660
_RS08165
SGL_RS08170: SGL_RS08170 - H(+)/Cl(-) exchange transporter ClcA, at 1,342,775 to 1,344,130
_RS08170
Position (kb)
1341
1342
1343 Strain fitness (log2 ratio)
-1
0
1
2 at 1340.765 kb on - strand, within SGL_RS08150 at 1340.815 kb on + strand, within SGL_RS08150 at 1340.828 kb on - strand, within SGL_RS08150 at 1340.870 kb on - strand, within SGL_RS08150 at 1340.920 kb on + strand, within SGL_RS08150 at 1341.246 kb on - strand, within SGL_RS08155 at 1341.480 kb on + strand, within SGL_RS08155 at 1341.524 kb on + strand, within SGL_RS08155 at 1341.735 kb on - strand, within SGL_RS08160 at 1341.852 kb on + strand, within SGL_RS08160 at 1341.876 kb on - strand, within SGL_RS08160 at 1341.882 kb on - strand, within SGL_RS08160 at 1341.916 kb on + strand, within SGL_RS08160 at 1341.998 kb on - strand, within SGL_RS08160 at 1342.004 kb on - strand, within SGL_RS08160 at 1342.196 kb on + strand, within SGL_RS08165 at 1342.204 kb on - strand, within SGL_RS08165 at 1342.221 kb on - strand, within SGL_RS08165 at 1342.225 kb on + strand, within SGL_RS08165 at 1342.228 kb on - strand, within SGL_RS08165 at 1342.281 kb on - strand, within SGL_RS08165 at 1342.378 kb on - strand, within SGL_RS08165 at 1342.463 kb on + strand, within SGL_RS08165 at 1342.649 kb on + strand at 1342.649 kb on + strand at 1342.689 kb on + strand at 1342.818 kb on + strand at 1342.835 kb on + strand at 1342.849 kb on + strand at 1342.861 kb on + strand at 1342.867 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,340,765 - SGL_RS08150 0.76 -0.2 1,340,815 + SGL_RS08150 0.79 +0.0 1,340,828 - SGL_RS08150 0.80 +0.6 1,340,870 - SGL_RS08150 0.83 -0.0 1,340,920 + SGL_RS08150 0.86 -0.4 1,341,246 - SGL_RS08155 0.23 +1.0 1,341,480 + SGL_RS08155 0.69 -1.0 1,341,524 + SGL_RS08155 0.78 -0.7 1,341,735 - SGL_RS08160 0.11 +1.7 1,341,852 + SGL_RS08160 0.43 +0.8 1,341,876 - SGL_RS08160 0.49 +2.4 1,341,882 - SGL_RS08160 0.51 +1.7 1,341,916 + SGL_RS08160 0.60 +0.2 1,341,998 - SGL_RS08160 0.82 -0.3 1,342,004 - SGL_RS08160 0.84 -0.5 1,342,196 + SGL_RS08165 0.24 -0.2 1,342,204 - SGL_RS08165 0.25 -0.4 1,342,221 - SGL_RS08165 0.28 -1.4 1,342,225 + SGL_RS08165 0.28 -1.1 1,342,228 - SGL_RS08165 0.29 -0.3 1,342,281 - SGL_RS08165 0.38 -0.1 1,342,378 - SGL_RS08165 0.54 -0.5 1,342,463 + SGL_RS08165 0.67 +0.1 1,342,649 + +0.6 1,342,649 + +0.4 1,342,689 + -0.3 1,342,818 + +0.0 1,342,835 + -0.6 1,342,849 + -0.3 1,342,861 + +0.1 1,342,867 - +0.2
Or see this region's nucleotide sequence