Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07550 and SGL_RS07555 are separated by 59 nucleotides SGL_RS07555 and SGL_RS07560 are separated by 137 nucleotides SGL_RS07560 and SGL_RS07565 are separated by 9 nucleotides SGL_RS07565 and SGL_RS19580 are separated by 45 nucleotides SGL_RS19580 and SGL_RS07570 are separated by 234 nucleotides
SGL_RS07550: SGL_RS07550 - AarF/ABC1/UbiB kinase family protein, at 1,220,473 to 1,222,230
_RS07550
SGL_RS07555: SGL_RS07555 - hypothetical protein, at 1,222,290 to 1,222,472
_RS07555
SGL_RS07560: SGL_RS07560 - thylakoid lumen protein, at 1,222,610 to 1,222,927
_RS07560
SGL_RS07565: SGL_RS07565 - hypothetical protein, at 1,222,937 to 1,223,209
_RS07565
SGL_RS19580: SGL_RS19580 - hypothetical protein, at 1,223,255 to 1,223,431
_RS19580
SGL_RS07570: SGL_RS07570 - ABC transporter permease, at 1,223,666 to 1,224,646
_RS07570
Position (kb)
1222
1223 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1221.717 kb on - strand, within SGL_RS07550 at 1221.722 kb on - strand, within SGL_RS07550 at 1221.727 kb on - strand, within SGL_RS07550 at 1221.778 kb on - strand, within SGL_RS07550 at 1221.804 kb on - strand, within SGL_RS07550 at 1221.926 kb on + strand, within SGL_RS07550 at 1221.942 kb on + strand, within SGL_RS07550 at 1221.942 kb on + strand, within SGL_RS07550 at 1222.415 kb on + strand, within SGL_RS07555 at 1222.613 kb on + strand at 1222.644 kb on + strand, within SGL_RS07560 at 1222.652 kb on - strand, within SGL_RS07560 at 1222.679 kb on - strand, within SGL_RS07560 at 1222.774 kb on - strand, within SGL_RS07560 at 1222.853 kb on + strand, within SGL_RS07560 at 1222.863 kb on - strand, within SGL_RS07560 at 1222.885 kb on + strand, within SGL_RS07560 at 1222.986 kb on + strand, within SGL_RS07565 at 1223.023 kb on - strand, within SGL_RS07565 at 1223.040 kb on + strand, within SGL_RS07565 at 1223.048 kb on - strand, within SGL_RS07565 at 1223.114 kb on + strand, within SGL_RS07565 at 1223.122 kb on - strand, within SGL_RS07565 at 1223.122 kb on - strand, within SGL_RS07565 at 1223.132 kb on + strand, within SGL_RS07565 at 1223.158 kb on - strand, within SGL_RS07565 at 1223.243 kb on - strand at 1223.344 kb on + strand, within SGL_RS19580 at 1223.344 kb on + strand, within SGL_RS19580 at 1223.356 kb on - strand, within SGL_RS19580 at 1223.534 kb on - strand at 1223.549 kb on + strand at 1223.609 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,221,717 - SGL_RS07550 0.71 -0.2 1,221,722 - SGL_RS07550 0.71 +0.1 1,221,727 - SGL_RS07550 0.71 -0.0 1,221,778 - SGL_RS07550 0.74 -0.3 1,221,804 - SGL_RS07550 0.76 +0.2 1,221,926 + SGL_RS07550 0.83 -0.2 1,221,942 + SGL_RS07550 0.84 -0.9 1,221,942 + SGL_RS07550 0.84 -0.5 1,222,415 + SGL_RS07555 0.68 -0.4 1,222,613 + +1.8 1,222,644 + SGL_RS07560 0.11 +0.4 1,222,652 - SGL_RS07560 0.13 +0.4 1,222,679 - SGL_RS07560 0.22 +2.8 1,222,774 - SGL_RS07560 0.52 +0.7 1,222,853 + SGL_RS07560 0.76 +0.9 1,222,863 - SGL_RS07560 0.80 +1.3 1,222,885 + SGL_RS07560 0.86 +1.2 1,222,986 + SGL_RS07565 0.18 -2.2 1,223,023 - SGL_RS07565 0.32 -3.9 1,223,040 + SGL_RS07565 0.38 -5.1 1,223,048 - SGL_RS07565 0.41 -5.0 1,223,114 + SGL_RS07565 0.65 -4.1 1,223,122 - SGL_RS07565 0.68 -4.9 1,223,122 - SGL_RS07565 0.68 -2.9 1,223,132 + SGL_RS07565 0.71 -4.6 1,223,158 - SGL_RS07565 0.81 -5.5 1,223,243 - -1.4 1,223,344 + SGL_RS19580 0.50 +1.1 1,223,344 + SGL_RS19580 0.50 +0.6 1,223,356 - SGL_RS19580 0.57 +1.1 1,223,534 - +0.2 1,223,549 + -0.9 1,223,609 + -0.6
Or see this region's nucleotide sequence