Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07485 and SGL_RS07490 are separated by 26 nucleotides SGL_RS07490 and SGL_RS07495 are separated by 51 nucleotides SGL_RS07495 and SGL_RS07500 are separated by 198 nucleotides SGL_RS07500 and SGL_RS07505 are separated by 12 nucleotides
SGL_RS07485: SGL_RS07485 - anthranilate phosphoribosyltransferase, at 1,206,947 to 1,207,993
_RS07485
SGL_RS07490: SGL_RS07490 - deoxyribose-phosphate aldolase, at 1,208,020 to 1,208,697
_RS07490
SGL_RS07495: SGL_RS07495 - GH116 family glycosyl hydrolase, at 1,208,749 to 1,211,202
_RS07495
SGL_RS07500: SGL_RS07500 - UPF0175 family protein, at 1,211,401 to 1,211,625
_RS07500
SGL_RS07505: SGL_RS07505 - Rpn family recombination-promoting nuclease/putative transposase, at 1,211,638 to 1,212,420
_RS07505
Position (kb)
1208
1209
1210
1211
1212 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 1208.114 kb on + strand, within SGL_RS07490 at 1208.145 kb on + strand, within SGL_RS07490 at 1208.158 kb on - strand, within SGL_RS07490 at 1208.193 kb on + strand, within SGL_RS07490 at 1208.198 kb on - strand, within SGL_RS07490 at 1208.236 kb on + strand, within SGL_RS07490 at 1208.314 kb on + strand, within SGL_RS07490 at 1208.336 kb on - strand, within SGL_RS07490 at 1208.452 kb on - strand, within SGL_RS07490 at 1208.562 kb on + strand, within SGL_RS07490 at 1208.590 kb on + strand, within SGL_RS07490 at 1208.615 kb on + strand, within SGL_RS07490 at 1208.622 kb on + strand, within SGL_RS07490 at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.648 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.900 kb on - strand at 1209.096 kb on - strand, within SGL_RS07495 at 1209.122 kb on + strand, within SGL_RS07495 at 1209.152 kb on + strand, within SGL_RS07495 at 1209.231 kb on + strand, within SGL_RS07495 at 1209.301 kb on + strand, within SGL_RS07495 at 1209.422 kb on - strand, within SGL_RS07495 at 1209.458 kb on - strand, within SGL_RS07495 at 1209.482 kb on - strand, within SGL_RS07495 at 1209.547 kb on + strand, within SGL_RS07495 at 1209.555 kb on - strand, within SGL_RS07495 at 1209.566 kb on - strand, within SGL_RS07495 at 1209.666 kb on + strand, within SGL_RS07495 at 1209.666 kb on + strand, within SGL_RS07495 at 1209.686 kb on + strand, within SGL_RS07495 at 1209.847 kb on + strand, within SGL_RS07495 at 1209.881 kb on - strand, within SGL_RS07495 at 1209.946 kb on + strand, within SGL_RS07495 at 1209.985 kb on - strand, within SGL_RS07495 at 1210.100 kb on + strand, within SGL_RS07495 at 1210.119 kb on - strand, within SGL_RS07495 at 1210.408 kb on + strand, within SGL_RS07495 at 1210.478 kb on + strand, within SGL_RS07495 at 1210.483 kb on + strand, within SGL_RS07495 at 1210.553 kb on - strand, within SGL_RS07495 at 1210.649 kb on - strand, within SGL_RS07495 at 1210.654 kb on + strand, within SGL_RS07495 at 1210.707 kb on - strand, within SGL_RS07495 at 1210.707 kb on - strand, within SGL_RS07495 at 1210.707 kb on - strand, within SGL_RS07495 at 1210.817 kb on + strand, within SGL_RS07495 at 1210.841 kb on - strand, within SGL_RS07495 at 1210.901 kb on - strand, within SGL_RS07495 at 1210.915 kb on + strand, within SGL_RS07495 at 1211.090 kb on - strand at 1211.094 kb on + strand at 1211.102 kb on - strand at 1211.120 kb on + strand at 1211.120 kb on + strand at 1211.120 kb on + strand at 1211.128 kb on - strand at 1211.128 kb on - strand at 1211.200 kb on - strand at 1211.224 kb on + strand at 1211.282 kb on + strand at 1211.282 kb on + strand at 1211.293 kb on + strand at 1211.301 kb on - strand at 1211.550 kb on - strand, within SGL_RS07500
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,208,114 + SGL_RS07490 0.14 -0.9 1,208,145 + SGL_RS07490 0.18 +0.3 1,208,158 - SGL_RS07490 0.20 +0.2 1,208,193 + SGL_RS07490 0.26 -0.1 1,208,198 - SGL_RS07490 0.26 +0.3 1,208,236 + SGL_RS07490 0.32 +0.1 1,208,314 + SGL_RS07490 0.43 -0.2 1,208,336 - SGL_RS07490 0.47 -0.4 1,208,452 - SGL_RS07490 0.64 +0.2 1,208,562 + SGL_RS07490 0.80 +0.3 1,208,590 + SGL_RS07490 0.84 +0.5 1,208,615 + SGL_RS07490 0.88 -0.0 1,208,622 + SGL_RS07490 0.89 +1.7 1,208,641 + -0.3 1,208,641 + -0.1 1,208,641 + +0.3 1,208,641 + -0.4 1,208,641 + -0.0 1,208,641 + +1.5 1,208,641 + +0.4 1,208,641 + -0.7 1,208,648 - +0.2 1,208,649 - +0.4 1,208,649 - -1.6 1,208,649 - +0.0 1,208,649 - -0.0 1,208,649 - -0.3 1,208,649 - +0.2 1,208,649 - +0.2 1,208,649 - -0.0 1,208,649 - +0.2 1,208,900 - +4.1 1,209,096 - SGL_RS07495 0.14 +3.1 1,209,122 + SGL_RS07495 0.15 +4.3 1,209,152 + SGL_RS07495 0.16 +3.0 1,209,231 + SGL_RS07495 0.20 +3.4 1,209,301 + SGL_RS07495 0.22 +3.7 1,209,422 - SGL_RS07495 0.27 +3.4 1,209,458 - SGL_RS07495 0.29 +3.2 1,209,482 - SGL_RS07495 0.30 +3.8 1,209,547 + SGL_RS07495 0.33 +5.2 1,209,555 - SGL_RS07495 0.33 +3.7 1,209,566 - SGL_RS07495 0.33 +2.9 1,209,666 + SGL_RS07495 0.37 +3.2 1,209,666 + SGL_RS07495 0.37 +3.3 1,209,686 + SGL_RS07495 0.38 +3.6 1,209,847 + SGL_RS07495 0.45 +3.6 1,209,881 - SGL_RS07495 0.46 +3.6 1,209,946 + SGL_RS07495 0.49 +3.4 1,209,985 - SGL_RS07495 0.50 +3.7 1,210,100 + SGL_RS07495 0.55 +3.9 1,210,119 - SGL_RS07495 0.56 +3.2 1,210,408 + SGL_RS07495 0.68 +3.1 1,210,478 + SGL_RS07495 0.70 +3.8 1,210,483 + SGL_RS07495 0.71 +3.5 1,210,553 - SGL_RS07495 0.74 +3.4 1,210,649 - SGL_RS07495 0.77 +2.7 1,210,654 + SGL_RS07495 0.78 +3.5 1,210,707 - SGL_RS07495 0.80 +3.9 1,210,707 - SGL_RS07495 0.80 +3.4 1,210,707 - SGL_RS07495 0.80 +3.4 1,210,817 + SGL_RS07495 0.84 +3.1 1,210,841 - SGL_RS07495 0.85 +2.5 1,210,901 - SGL_RS07495 0.88 +3.3 1,210,915 + SGL_RS07495 0.88 +3.3 1,211,090 - +3.2 1,211,094 + +3.3 1,211,102 - +4.0 1,211,120 + +3.4 1,211,120 + +3.6 1,211,120 + +3.1 1,211,128 - +3.7 1,211,128 - +3.2 1,211,200 - +2.8 1,211,224 + +0.8 1,211,282 + -1.5 1,211,282 + +0.0 1,211,293 + -0.6 1,211,301 - +0.3 1,211,550 - SGL_RS07500 0.66 -0.0
Or see this region's nucleotide sequence