Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07280 and SGL_RS07285 are separated by 119 nucleotides SGL_RS07285 and SGL_RS07290 overlap by 18 nucleotides SGL_RS07290 and SGL_RS07295 are separated by 180 nucleotides
SGL_RS07280: SGL_RS07280 - excinuclease ABC subunit UvrC, at 1,157,029 to 1,158,909
_RS07280
SGL_RS07285: SGL_RS07285 - N-acetylmuramoyl-L-alanine amidase, at 1,159,029 to 1,160,804
_RS07285
SGL_RS07290: SGL_RS07290 - M67 family metallopeptidase, at 1,160,787 to 1,161,260
_RS07290
SGL_RS07295: SGL_RS07295 - hypothetical protein, at 1,161,441 to 1,161,824
_RS07295
Position (kb)
1159
1160
1161 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1158.470 kb on + strand, within SGL_RS07280 at 1158.691 kb on + strand, within SGL_RS07280 at 1158.719 kb on + strand, within SGL_RS07280 at 1159.082 kb on + strand at 1159.100 kb on - strand at 1159.482 kb on + strand, within SGL_RS07285 at 1159.502 kb on - strand, within SGL_RS07285 at 1159.594 kb on - strand, within SGL_RS07285 at 1159.594 kb on - strand, within SGL_RS07285 at 1159.747 kb on + strand, within SGL_RS07285 at 1159.767 kb on + strand, within SGL_RS07285 at 1159.844 kb on - strand, within SGL_RS07285 at 1159.876 kb on + strand, within SGL_RS07285 at 1159.941 kb on - strand, within SGL_RS07285 at 1160.439 kb on + strand, within SGL_RS07285 at 1160.496 kb on + strand, within SGL_RS07285 at 1160.596 kb on - strand, within SGL_RS07285 at 1160.862 kb on - strand, within SGL_RS07290 at 1160.931 kb on - strand, within SGL_RS07290 at 1161.040 kb on - strand, within SGL_RS07290 at 1161.118 kb on - strand, within SGL_RS07290 at 1161.538 kb on + strand, within SGL_RS07295 at 1161.572 kb on - strand, within SGL_RS07295 at 1161.572 kb on - strand, within SGL_RS07295 at 1161.584 kb on + strand, within SGL_RS07295 at 1161.732 kb on + strand, within SGL_RS07295 at 1161.732 kb on + strand, within SGL_RS07295 at 1161.740 kb on - strand, within SGL_RS07295 at 1161.758 kb on - strand, within SGL_RS07295 at 1161.758 kb on - strand, within SGL_RS07295
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,158,470 + SGL_RS07280 0.77 +0.2 1,158,691 + SGL_RS07280 0.88 -0.1 1,158,719 + SGL_RS07280 0.90 +0.9 1,159,082 + -3.7 1,159,100 - -3.3 1,159,482 + SGL_RS07285 0.26 -4.7 1,159,502 - SGL_RS07285 0.27 -5.1 1,159,594 - SGL_RS07285 0.32 -4.4 1,159,594 - SGL_RS07285 0.32 -5.7 1,159,747 + SGL_RS07285 0.40 -6.6 1,159,767 + SGL_RS07285 0.42 -5.1 1,159,844 - SGL_RS07285 0.46 -5.4 1,159,876 + SGL_RS07285 0.48 -6.6 1,159,941 - SGL_RS07285 0.51 -4.2 1,160,439 + SGL_RS07285 0.79 -2.0 1,160,496 + SGL_RS07285 0.83 -5.2 1,160,596 - SGL_RS07285 0.88 -3.8 1,160,862 - SGL_RS07290 0.16 -0.1 1,160,931 - SGL_RS07290 0.30 +0.3 1,161,040 - SGL_RS07290 0.53 -0.3 1,161,118 - SGL_RS07290 0.70 -0.3 1,161,538 + SGL_RS07295 0.25 +0.8 1,161,572 - SGL_RS07295 0.34 +0.2 1,161,572 - SGL_RS07295 0.34 +0.5 1,161,584 + SGL_RS07295 0.37 +0.2 1,161,732 + SGL_RS07295 0.76 +0.6 1,161,732 + SGL_RS07295 0.76 +0.4 1,161,740 - SGL_RS07295 0.78 +0.1 1,161,758 - SGL_RS07295 0.83 +0.1 1,161,758 - SGL_RS07295 0.83 +1.4
Or see this region's nucleotide sequence