Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07165 and SGL_RS07170 are separated by 97 nucleotides SGL_RS07170 and SGL_RS07175 are separated by 93 nucleotides SGL_RS07175 and SGL_RS07180 are separated by 22 nucleotides
SGL_RS07165: SGL_RS07165 - M23 family metallopeptidase, at 1,137,216 to 1,138,112
_RS07165
SGL_RS07170: SGL_RS07170 - glycine cleavage system protein GcvH, at 1,138,210 to 1,138,608
_RS07170
SGL_RS07175: SGL_RS07175 - NFACT family protein, at 1,138,702 to 1,140,474
_RS07175
SGL_RS07180: SGL_RS07180 - carboxynorspermidine decarboxylase, at 1,140,497 to 1,141,645
_RS07180
Position (kb)
1138
1139
1140
1141 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1138.309 kb on + strand, within SGL_RS07170 at 1138.385 kb on - strand, within SGL_RS07170 at 1138.414 kb on + strand, within SGL_RS07170 at 1138.578 kb on + strand at 1138.589 kb on + strand at 1138.623 kb on + strand at 1138.623 kb on + strand at 1138.723 kb on + strand at 1138.746 kb on + strand at 1138.804 kb on + strand at 1138.877 kb on + strand at 1138.947 kb on + strand, within SGL_RS07175 at 1138.947 kb on + strand, within SGL_RS07175 at 1138.955 kb on - strand, within SGL_RS07175 at 1139.046 kb on - strand, within SGL_RS07175 at 1139.138 kb on - strand, within SGL_RS07175 at 1139.192 kb on - strand, within SGL_RS07175 at 1139.317 kb on - strand, within SGL_RS07175 at 1139.477 kb on - strand, within SGL_RS07175 at 1139.508 kb on + strand, within SGL_RS07175 at 1139.689 kb on - strand, within SGL_RS07175 at 1139.735 kb on - strand, within SGL_RS07175 at 1139.767 kb on + strand, within SGL_RS07175 at 1139.775 kb on - strand, within SGL_RS07175 at 1139.937 kb on - strand, within SGL_RS07175 at 1140.095 kb on + strand, within SGL_RS07175 at 1140.113 kb on + strand, within SGL_RS07175 at 1140.121 kb on - strand, within SGL_RS07175 at 1140.233 kb on + strand, within SGL_RS07175 at 1140.241 kb on - strand, within SGL_RS07175 at 1140.343 kb on - strand at 1140.372 kb on - strand at 1140.392 kb on - strand at 1140.460 kb on - strand at 1140.594 kb on + strand at 1140.594 kb on + strand at 1140.688 kb on - strand, within SGL_RS07180 at 1140.997 kb on - strand, within SGL_RS07180 at 1141.026 kb on + strand, within SGL_RS07180 at 1141.145 kb on - strand, within SGL_RS07180 at 1141.325 kb on - strand, within SGL_RS07180 at 1141.457 kb on + strand, within SGL_RS07180
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,138,309 + SGL_RS07170 0.25 -4.7 1,138,385 - SGL_RS07170 0.44 -1.2 1,138,414 + SGL_RS07170 0.51 -2.0 1,138,578 + +1.4 1,138,589 + +1.0 1,138,623 + +0.6 1,138,623 + -0.1 1,138,723 + +1.6 1,138,746 + +1.7 1,138,804 + -0.2 1,138,877 + +1.5 1,138,947 + SGL_RS07175 0.14 +1.4 1,138,947 + SGL_RS07175 0.14 +1.1 1,138,955 - SGL_RS07175 0.14 +1.5 1,139,046 - SGL_RS07175 0.19 +1.7 1,139,138 - SGL_RS07175 0.25 +1.8 1,139,192 - SGL_RS07175 0.28 +1.7 1,139,317 - SGL_RS07175 0.35 +1.5 1,139,477 - SGL_RS07175 0.44 +1.5 1,139,508 + SGL_RS07175 0.45 +1.1 1,139,689 - SGL_RS07175 0.56 +1.6 1,139,735 - SGL_RS07175 0.58 +1.6 1,139,767 + SGL_RS07175 0.60 +0.2 1,139,775 - SGL_RS07175 0.61 +1.5 1,139,937 - SGL_RS07175 0.70 +1.2 1,140,095 + SGL_RS07175 0.79 +2.2 1,140,113 + SGL_RS07175 0.80 +3.5 1,140,121 - SGL_RS07175 0.80 +2.2 1,140,233 + SGL_RS07175 0.86 +1.1 1,140,241 - SGL_RS07175 0.87 +1.5 1,140,343 - +1.3 1,140,372 - +1.6 1,140,392 - +0.9 1,140,460 - -0.1 1,140,594 + -0.2 1,140,594 + -2.4 1,140,688 - SGL_RS07180 0.17 -0.8 1,140,997 - SGL_RS07180 0.44 -0.2 1,141,026 + SGL_RS07180 0.46 -2.3 1,141,145 - SGL_RS07180 0.56 -2.0 1,141,325 - SGL_RS07180 0.72 -0.5 1,141,457 + SGL_RS07180 0.84 +0.8
Or see this region's nucleotide sequence