Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06775 and SGL_RS06780 overlap by 4 nucleotides SGL_RS06780 and SGL_RS06785 are separated by 500 nucleotides SGL_RS06785 and SGL_RS06790 are separated by 52 nucleotides SGL_RS06790 and SGL_RS06795 are separated by 82 nucleotides SGL_RS06795 and SGL_RS06800 are separated by 80 nucleotides
SGL_RS06775: SGL_RS06775 - type II toxin-antitoxin system PemK/MazF family toxin, at 1,048,406 to 1,048,753
_RS06775
SGL_RS06780: SGL_RS06780 - hypothetical protein, at 1,048,750 to 1,049,016
_RS06780
SGL_RS06785: SGL_RS06785 - alpha/beta hydrolase, at 1,049,517 to 1,050,407
_RS06785
SGL_RS06790: SGL_RS06790 - hypothetical protein, at 1,050,460 to 1,050,729
_RS06790
SGL_RS06795: SGL_RS06795 - glutaredoxin 3, at 1,050,812 to 1,051,078
_RS06795
SGL_RS06800: SGL_RS06800 - glutathione synthase, at 1,051,159 to 1,052,121
_RS06800
Position (kb)
1049
1050
1051 Strain fitness (log2 ratio)
-1
0
1
2 at 1048.564 kb on - strand, within SGL_RS06775 at 1048.615 kb on + strand, within SGL_RS06775 at 1048.854 kb on + strand, within SGL_RS06780 at 1048.861 kb on + strand, within SGL_RS06780 at 1048.862 kb on - strand, within SGL_RS06780 at 1048.935 kb on + strand, within SGL_RS06780 at 1049.024 kb on - strand at 1049.026 kb on - strand at 1049.212 kb on + strand at 1049.641 kb on - strand, within SGL_RS06785 at 1049.751 kb on + strand, within SGL_RS06785 at 1049.763 kb on + strand, within SGL_RS06785 at 1049.794 kb on + strand, within SGL_RS06785 at 1049.794 kb on + strand, within SGL_RS06785 at 1049.841 kb on + strand, within SGL_RS06785 at 1049.864 kb on + strand, within SGL_RS06785 at 1049.872 kb on - strand, within SGL_RS06785 at 1049.945 kb on + strand, within SGL_RS06785 at 1050.011 kb on - strand, within SGL_RS06785 at 1050.252 kb on + strand, within SGL_RS06785 at 1050.263 kb on + strand, within SGL_RS06785 at 1050.302 kb on + strand, within SGL_RS06785 at 1050.389 kb on + strand at 1050.390 kb on + strand at 1050.397 kb on - strand at 1050.397 kb on - strand at 1050.398 kb on - strand at 1050.409 kb on + strand at 1050.409 kb on - strand at 1050.645 kb on + strand, within SGL_RS06790 at 1050.733 kb on - strand at 1051.037 kb on - strand, within SGL_RS06795 at 1051.082 kb on + strand at 1051.146 kb on + strand at 1051.217 kb on - strand at 1051.310 kb on - strand, within SGL_RS06800 at 1051.374 kb on + strand, within SGL_RS06800 at 1051.389 kb on + strand, within SGL_RS06800 at 1051.389 kb on + strand, within SGL_RS06800
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,048,564 - SGL_RS06775 0.45 -0.2 1,048,615 + SGL_RS06775 0.60 +0.2 1,048,854 + SGL_RS06780 0.39 +0.3 1,048,861 + SGL_RS06780 0.42 +0.8 1,048,862 - SGL_RS06780 0.42 -0.6 1,048,935 + SGL_RS06780 0.69 -0.4 1,049,024 - -0.3 1,049,026 - +0.2 1,049,212 + -0.8 1,049,641 - SGL_RS06785 0.14 -0.4 1,049,751 + SGL_RS06785 0.26 +0.5 1,049,763 + SGL_RS06785 0.28 +0.0 1,049,794 + SGL_RS06785 0.31 +0.8 1,049,794 + SGL_RS06785 0.31 -1.5 1,049,841 + SGL_RS06785 0.36 -0.4 1,049,864 + SGL_RS06785 0.39 +1.5 1,049,872 - SGL_RS06785 0.40 +0.1 1,049,945 + SGL_RS06785 0.48 -0.5 1,050,011 - SGL_RS06785 0.55 +0.2 1,050,252 + SGL_RS06785 0.82 +0.3 1,050,263 + SGL_RS06785 0.84 +0.6 1,050,302 + SGL_RS06785 0.88 +0.4 1,050,389 + +0.1 1,050,390 + +0.4 1,050,397 - +0.4 1,050,397 - +0.0 1,050,398 - +0.4 1,050,409 + +0.1 1,050,409 - +0.0 1,050,645 + SGL_RS06790 0.69 +0.7 1,050,733 - -0.0 1,051,037 - SGL_RS06795 0.84 -1.2 1,051,082 + -0.5 1,051,146 + +0.7 1,051,217 - +2.2 1,051,310 - SGL_RS06800 0.16 +0.6 1,051,374 + SGL_RS06800 0.22 +0.4 1,051,389 + SGL_RS06800 0.24 +0.6 1,051,389 + SGL_RS06800 0.24 +0.1
Or see this region's nucleotide sequence