Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06730 and SGL_RS06735 are separated by 131 nucleotides SGL_RS06735 and SGL_RS06740 are separated by 51 nucleotides SGL_RS06740 and SGL_RS06745 are separated by 45 nucleotides SGL_RS06745 and SGL_RS06750 are separated by 36 nucleotides SGL_RS06750 and SGL_RS06755 are separated by 66 nucleotides
SGL_RS06730: SGL_RS06730 - succinate dehydrogenase/fumarate reductase flavoprotein subunit, at 1,040,531 to 1,042,258
_RS06730
SGL_RS06735: SGL_RS06735 - PCP reductase family protein, at 1,042,390 to 1,042,557
_RS06735
SGL_RS06740: SGL_RS06740 - histidine triad nucleotide-binding protein, at 1,042,609 to 1,042,953
_RS06740
SGL_RS06745: SGL_RS06745 - alpha/beta fold hydrolase, at 1,042,999 to 1,043,880
_RS06745
SGL_RS06750: SGL_RS06750 - DUF192 domain-containing protein, at 1,043,917 to 1,044,450
_RS06750
SGL_RS06755: SGL_RS06755 - bacterioferritin-associated ferredoxin, at 1,044,517 to 1,044,693
_RS06755
Position (kb)
1042
1043
1044 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1042.046 kb on - strand, within SGL_RS06730 at 1042.061 kb on - strand, within SGL_RS06730 at 1042.170 kb on + strand at 1042.668 kb on - strand, within SGL_RS06740 at 1042.690 kb on + strand, within SGL_RS06740 at 1042.806 kb on + strand, within SGL_RS06740 at 1042.839 kb on - strand, within SGL_RS06740 at 1042.846 kb on - strand, within SGL_RS06740 at 1042.955 kb on + strand at 1043.126 kb on + strand, within SGL_RS06745 at 1043.170 kb on + strand, within SGL_RS06745 at 1043.190 kb on - strand, within SGL_RS06745 at 1043.200 kb on + strand, within SGL_RS06745 at 1043.217 kb on - strand, within SGL_RS06745 at 1043.244 kb on - strand, within SGL_RS06745 at 1043.381 kb on + strand, within SGL_RS06745 at 1043.391 kb on + strand, within SGL_RS06745 at 1043.473 kb on + strand, within SGL_RS06745 at 1043.481 kb on - strand, within SGL_RS06745 at 1043.535 kb on - strand, within SGL_RS06745 at 1043.631 kb on - strand, within SGL_RS06745 at 1043.666 kb on - strand, within SGL_RS06745 at 1043.666 kb on - strand, within SGL_RS06745 at 1043.778 kb on + strand, within SGL_RS06745 at 1043.808 kb on + strand at 1043.870 kb on + strand at 1043.883 kb on - strand at 1043.883 kb on - strand at 1043.984 kb on + strand, within SGL_RS06750 at 1043.992 kb on - strand, within SGL_RS06750 at 1044.029 kb on + strand, within SGL_RS06750 at 1044.032 kb on - strand, within SGL_RS06750 at 1044.091 kb on + strand, within SGL_RS06750 at 1044.204 kb on + strand, within SGL_RS06750 at 1044.306 kb on + strand, within SGL_RS06750 at 1044.369 kb on - strand, within SGL_RS06750 at 1044.486 kb on - strand at 1044.559 kb on + strand, within SGL_RS06755 at 1044.610 kb on - strand, within SGL_RS06755 at 1044.623 kb on - strand, within SGL_RS06755 at 1044.625 kb on + strand, within SGL_RS06755 at 1044.634 kb on - strand, within SGL_RS06755 at 1044.634 kb on - strand, within SGL_RS06755 at 1044.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,042,046 - SGL_RS06730 0.88 -0.4 1,042,061 - SGL_RS06730 0.89 +0.4 1,042,170 + -0.5 1,042,668 - SGL_RS06740 0.17 +0.3 1,042,690 + SGL_RS06740 0.23 +0.0 1,042,806 + SGL_RS06740 0.57 +1.5 1,042,839 - SGL_RS06740 0.67 +0.0 1,042,846 - SGL_RS06740 0.69 -0.4 1,042,955 + +0.0 1,043,126 + SGL_RS06745 0.14 +0.5 1,043,170 + SGL_RS06745 0.19 -2.9 1,043,190 - SGL_RS06745 0.22 -0.1 1,043,200 + SGL_RS06745 0.23 -0.1 1,043,217 - SGL_RS06745 0.25 +0.5 1,043,244 - SGL_RS06745 0.28 +0.0 1,043,381 + SGL_RS06745 0.43 -0.3 1,043,391 + SGL_RS06745 0.44 +0.0 1,043,473 + SGL_RS06745 0.54 -0.4 1,043,481 - SGL_RS06745 0.55 +0.5 1,043,535 - SGL_RS06745 0.61 +0.0 1,043,631 - SGL_RS06745 0.72 -0.3 1,043,666 - SGL_RS06745 0.76 -0.3 1,043,666 - SGL_RS06745 0.76 -1.2 1,043,778 + SGL_RS06745 0.88 +0.3 1,043,808 + +0.0 1,043,870 + +0.2 1,043,883 - -0.4 1,043,883 - -1.1 1,043,984 + SGL_RS06750 0.13 -2.7 1,043,992 - SGL_RS06750 0.14 -3.4 1,044,029 + SGL_RS06750 0.21 -5.2 1,044,032 - SGL_RS06750 0.22 -3.1 1,044,091 + SGL_RS06750 0.33 -5.3 1,044,204 + SGL_RS06750 0.54 -5.0 1,044,306 + SGL_RS06750 0.73 -3.7 1,044,369 - SGL_RS06750 0.85 -3.8 1,044,486 - -0.4 1,044,559 + SGL_RS06755 0.24 +0.7 1,044,610 - SGL_RS06755 0.53 +0.8 1,044,623 - SGL_RS06755 0.60 -0.3 1,044,625 + SGL_RS06755 0.61 +0.4 1,044,634 - SGL_RS06755 0.66 -0.0 1,044,634 - SGL_RS06755 0.66 +0.0 1,044,859 - +0.4
Or see this region's nucleotide sequence