Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06660 and SGL_RS06665 are separated by 165 nucleotides SGL_RS06665 and SGL_RS06670 are separated by 172 nucleotides
SGL_RS06660: SGL_RS06660 - DNA helicase PcrA, at 1,022,278 to 1,024,659
_RS06660
SGL_RS06665: SGL_RS06665 - bifunctional nuclease family protein, at 1,024,825 to 1,025,346
_RS06665
SGL_RS06670: SGL_RS06670 - TIGR01777 family oxidoreductase, at 1,025,519 to 1,026,442
_RS06670
Position (kb)
1024
1025
1026 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 1024.403 kb on + strand, within SGL_RS06660 at 1024.411 kb on - strand, within SGL_RS06660 at 1024.411 kb on - strand, within SGL_RS06660 at 1024.524 kb on + strand at 1024.559 kb on + strand at 1024.624 kb on - strand at 1024.626 kb on + strand at 1024.626 kb on + strand at 1024.634 kb on - strand at 1024.671 kb on + strand at 1024.793 kb on + strand at 1024.866 kb on - strand at 1024.879 kb on - strand, within SGL_RS06665 at 1025.070 kb on + strand, within SGL_RS06665 at 1025.070 kb on + strand, within SGL_RS06665 at 1025.072 kb on + strand, within SGL_RS06665 at 1025.075 kb on + strand, within SGL_RS06665 at 1025.075 kb on + strand, within SGL_RS06665 at 1025.078 kb on - strand, within SGL_RS06665 at 1025.078 kb on - strand, within SGL_RS06665 at 1025.276 kb on + strand, within SGL_RS06665 at 1025.420 kb on + strand at 1025.421 kb on - strand at 1025.428 kb on - strand at 1025.590 kb on - strand at 1025.616 kb on + strand, within SGL_RS06670 at 1025.628 kb on - strand, within SGL_RS06670 at 1025.703 kb on + strand, within SGL_RS06670 at 1025.974 kb on + strand, within SGL_RS06670 at 1026.038 kb on + strand, within SGL_RS06670 at 1026.082 kb on + strand, within SGL_RS06670 at 1026.114 kb on + strand, within SGL_RS06670 at 1026.225 kb on - strand, within SGL_RS06670 at 1026.263 kb on + strand, within SGL_RS06670
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,024,403 + SGL_RS06660 0.89 +0.5 1,024,411 - SGL_RS06660 0.90 +0.3 1,024,411 - SGL_RS06660 0.90 +0.7 1,024,524 + -0.6 1,024,559 + -0.8 1,024,624 - +0.5 1,024,626 + -0.1 1,024,626 + +0.9 1,024,634 - +0.7 1,024,671 + +0.3 1,024,793 + +0.0 1,024,866 - +5.3 1,024,879 - SGL_RS06665 0.10 +5.8 1,025,070 + SGL_RS06665 0.47 +5.8 1,025,070 + SGL_RS06665 0.47 +5.5 1,025,072 + SGL_RS06665 0.47 +6.4 1,025,075 + SGL_RS06665 0.48 +5.5 1,025,075 + SGL_RS06665 0.48 +5.7 1,025,078 - SGL_RS06665 0.48 +6.0 1,025,078 - SGL_RS06665 0.48 +5.6 1,025,276 + SGL_RS06665 0.86 +5.1 1,025,420 + -0.4 1,025,421 - -0.4 1,025,428 - +0.2 1,025,590 - +0.5 1,025,616 + SGL_RS06670 0.10 +1.9 1,025,628 - SGL_RS06670 0.12 +0.8 1,025,703 + SGL_RS06670 0.20 +0.9 1,025,974 + SGL_RS06670 0.49 +1.0 1,026,038 + SGL_RS06670 0.56 +0.8 1,026,082 + SGL_RS06670 0.61 -0.3 1,026,114 + SGL_RS06670 0.64 -0.2 1,026,225 - SGL_RS06670 0.76 +0.3 1,026,263 + SGL_RS06670 0.81 -1.1
Or see this region's nucleotide sequence