Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06230 and SGL_RS06235 are separated by 143 nucleotides SGL_RS06235 and SGL_RS06240 overlap by 4 nucleotides SGL_RS06240 and SGL_RS06245 are separated by 55 nucleotides
SGL_RS06230: SGL_RS06230 - 50S ribosomal protein L19, at 926,860 to 927,222
_RS06230
SGL_RS06235: SGL_RS06235 - hypothetical protein, at 927,366 to 927,758
_RS06235
SGL_RS06240: SGL_RS06240 - alpha/beta fold hydrolase, at 927,755 to 928,750
_RS06240
SGL_RS06245: SGL_RS06245 - late competence development ComFB family protein, at 928,806 to 929,885
_RS06245
Position (kb) 927
928
929 Strain fitness (log2 ratio) -2
-1
0
1 at 926.829 kb on + strand at 926.856 kb on - strand at 927.381 kb on - strand at 927.541 kb on - strand, within SGL_RS06235 at 927.674 kb on - strand, within SGL_RS06235 at 927.879 kb on + strand, within SGL_RS06240 at 927.905 kb on + strand, within SGL_RS06240 at 927.924 kb on + strand, within SGL_RS06240 at 928.046 kb on + strand, within SGL_RS06240 at 928.161 kb on + strand, within SGL_RS06240 at 928.296 kb on - strand, within SGL_RS06240 at 928.385 kb on - strand, within SGL_RS06240 at 928.385 kb on - strand, within SGL_RS06240 at 928.480 kb on + strand, within SGL_RS06240 at 929.012 kb on + strand, within SGL_RS06245 at 929.404 kb on - strand, within SGL_RS06245
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 926,829 + -2.2 926,856 - +0.1 927,381 - +0.0 927,541 - SGL_RS06235 0.45 -0.0 927,674 - SGL_RS06235 0.78 -0.2 927,879 + SGL_RS06240 0.12 -0.4 927,905 + SGL_RS06240 0.15 -0.9 927,924 + SGL_RS06240 0.17 -0.4 928,046 + SGL_RS06240 0.29 -0.7 928,161 + SGL_RS06240 0.41 +1.2 928,296 - SGL_RS06240 0.54 -0.8 928,385 - SGL_RS06240 0.63 -0.4 928,385 - SGL_RS06240 0.63 -0.1 928,480 + SGL_RS06240 0.73 -1.1 929,012 + SGL_RS06245 0.19 -1.0 929,404 - SGL_RS06245 0.55 -0.2
Or see this region's nucleotide sequence