Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05490 and SGL_RS05495 are separated by 208 nucleotides SGL_RS05495 and SGL_RS05500 are separated by 128 nucleotides SGL_RS05500 and SGL_RS05505 are separated by 681 nucleotides
SGL_RS05490: SGL_RS05490 - hypothetical protein, at 776,058 to 776,342
_RS05490
SGL_RS05495: SGL_RS05495 - hypothetical protein, at 776,551 to 776,937
_RS05495
SGL_RS05500: SGL_RS05500 - ribonuclease R, at 777,066 to 779,411
_RS05500
SGL_RS05505: SGL_RS05505 - TIGR03279 family radical SAM protein, at 780,093 to 781,448
_RS05505
Position (kb)
777
778
779
780 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 776.152 kb on + strand, within SGL_RS05490 at 776.285 kb on + strand, within SGL_RS05490 at 776.628 kb on + strand, within SGL_RS05495 at 776.628 kb on + strand, within SGL_RS05495 at 776.628 kb on + strand, within SGL_RS05495 at 776.636 kb on - strand, within SGL_RS05495 at 776.641 kb on - strand, within SGL_RS05495 at 776.736 kb on - strand, within SGL_RS05495 at 776.797 kb on + strand, within SGL_RS05495 at 777.055 kb on + strand at 777.247 kb on - strand at 777.366 kb on + strand, within SGL_RS05500 at 777.381 kb on - strand, within SGL_RS05500 at 777.682 kb on + strand, within SGL_RS05500 at 777.688 kb on - strand, within SGL_RS05500 at 777.688 kb on - strand, within SGL_RS05500 at 777.699 kb on - strand, within SGL_RS05500 at 777.707 kb on + strand, within SGL_RS05500 at 777.745 kb on - strand, within SGL_RS05500 at 777.845 kb on - strand, within SGL_RS05500 at 778.012 kb on + strand, within SGL_RS05500 at 778.012 kb on + strand, within SGL_RS05500 at 778.238 kb on - strand, within SGL_RS05500 at 778.321 kb on + strand, within SGL_RS05500 at 778.321 kb on + strand, within SGL_RS05500 at 778.328 kb on + strand, within SGL_RS05500 at 778.329 kb on - strand, within SGL_RS05500 at 778.418 kb on + strand, within SGL_RS05500 at 778.420 kb on + strand, within SGL_RS05500 at 778.598 kb on + strand, within SGL_RS05500 at 778.744 kb on - strand, within SGL_RS05500 at 778.853 kb on - strand, within SGL_RS05500 at 778.866 kb on - strand, within SGL_RS05500 at 778.908 kb on + strand, within SGL_RS05500 at 778.916 kb on + strand, within SGL_RS05500 at 779.031 kb on + strand, within SGL_RS05500 at 779.079 kb on - strand, within SGL_RS05500 at 779.216 kb on + strand at 779.229 kb on - strand at 779.295 kb on + strand at 779.313 kb on - strand at 779.487 kb on - strand at 779.516 kb on + strand at 779.665 kb on + strand at 779.730 kb on + strand at 779.730 kb on + strand at 779.730 kb on + strand at 779.730 kb on + strand at 779.738 kb on - strand at 779.738 kb on - strand at 779.738 kb on - strand at 779.738 kb on - strand at 779.738 kb on - strand at 779.836 kb on + strand at 779.893 kb on - strand at 779.898 kb on - strand at 779.902 kb on + strand at 779.957 kb on + strand at 779.987 kb on - strand at 780.046 kb on + strand at 780.064 kb on - strand at 780.069 kb on - strand at 780.084 kb on - strand at 780.168 kb on + strand at 780.203 kb on + strand at 780.207 kb on - strand at 780.274 kb on - strand, within SGL_RS05505 at 780.282 kb on - strand, within SGL_RS05505 at 780.328 kb on - strand, within SGL_RS05505 at 780.345 kb on + strand, within SGL_RS05505 at 780.345 kb on + strand, within SGL_RS05505 at 780.353 kb on - strand, within SGL_RS05505
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 776,152 + SGL_RS05490 0.33 -0.1 776,285 + SGL_RS05490 0.80 -0.2 776,628 + SGL_RS05495 0.20 -0.6 776,628 + SGL_RS05495 0.20 +0.3 776,628 + SGL_RS05495 0.20 +0.5 776,636 - SGL_RS05495 0.22 +0.6 776,641 - SGL_RS05495 0.23 +0.1 776,736 - SGL_RS05495 0.48 +0.1 776,797 + SGL_RS05495 0.64 -0.5 777,055 + -0.6 777,247 - -0.2 777,366 + SGL_RS05500 0.13 -2.0 777,381 - SGL_RS05500 0.13 -0.8 777,682 + SGL_RS05500 0.26 +0.6 777,688 - SGL_RS05500 0.27 +1.0 777,688 - SGL_RS05500 0.27 +0.9 777,699 - SGL_RS05500 0.27 +2.1 777,707 + SGL_RS05500 0.27 +0.9 777,745 - SGL_RS05500 0.29 +0.8 777,845 - SGL_RS05500 0.33 +1.0 778,012 + SGL_RS05500 0.40 +1.3 778,012 + SGL_RS05500 0.40 +1.7 778,238 - SGL_RS05500 0.50 +1.1 778,321 + SGL_RS05500 0.53 +2.1 778,321 + SGL_RS05500 0.53 +0.5 778,328 + SGL_RS05500 0.54 -0.8 778,329 - SGL_RS05500 0.54 +0.6 778,418 + SGL_RS05500 0.58 +1.3 778,420 + SGL_RS05500 0.58 +1.4 778,598 + SGL_RS05500 0.65 +1.0 778,744 - SGL_RS05500 0.72 +0.8 778,853 - SGL_RS05500 0.76 +1.6 778,866 - SGL_RS05500 0.77 +0.4 778,908 + SGL_RS05500 0.79 +1.3 778,916 + SGL_RS05500 0.79 +1.0 779,031 + SGL_RS05500 0.84 +1.2 779,079 - SGL_RS05500 0.86 +1.4 779,216 + +1.5 779,229 - +1.6 779,295 + +1.3 779,313 - +1.5 779,487 - +1.5 779,516 + +0.9 779,665 + +0.9 779,730 + +0.0 779,730 + +0.0 779,730 + +1.0 779,730 + +0.3 779,738 - +0.2 779,738 - +0.5 779,738 - -0.1 779,738 - +0.2 779,738 - +0.4 779,836 + -0.2 779,893 - +0.6 779,898 - +0.4 779,902 + +0.2 779,957 + -1.9 779,987 - -0.4 780,046 + +1.6 780,064 - +0.2 780,069 - +0.5 780,084 - +0.3 780,168 + +0.6 780,203 + +0.2 780,207 - -0.9 780,274 - SGL_RS05505 0.13 +0.1 780,282 - SGL_RS05505 0.14 +0.3 780,328 - SGL_RS05505 0.17 +0.4 780,345 + SGL_RS05505 0.19 +0.3 780,345 + SGL_RS05505 0.19 +0.5 780,353 - SGL_RS05505 0.19 +0.2
Or see this region's nucleotide sequence