Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05455 and SGL_RS05460 overlap by 4 nucleotides SGL_RS05460 and SGL_RS05465 are separated by 39 nucleotides SGL_RS05465 and SGL_RS05470 are separated by 169 nucleotides
SGL_RS05455: SGL_RS05455 - radical SAM family heme chaperone HemW, at 769,053 to 770,291
_RS05455
SGL_RS05460: SGL_RS05460 - nicotinate-nucleotide adenylyltransferase, at 770,288 to 770,890
_RS05460
SGL_RS05465: SGL_RS05465 - hypothetical protein, at 770,930 to 771,481
_RS05465
SGL_RS05470: SGL_RS05470 - dihydropteroate synthase, at 771,651 to 772,520
_RS05470
Position (kb) 770
771
772 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 770.113 kb on - strand, within SGL_RS05455 at 770.391 kb on + strand, within SGL_RS05460 at 771.248 kb on - strand, within SGL_RS05465 at 771.265 kb on - strand, within SGL_RS05465 at 771.332 kb on - strand, within SGL_RS05465 at 771.351 kb on + strand, within SGL_RS05465 at 771.351 kb on + strand, within SGL_RS05465 at 771.426 kb on - strand at 771.547 kb on - strand at 771.566 kb on + strand at 771.574 kb on - strand at 771.597 kb on + strand at 771.648 kb on + strand at 771.663 kb on + strand at 771.710 kb on - strand at 771.769 kb on + strand, within SGL_RS05470 at 771.769 kb on + strand, within SGL_RS05470 at 771.831 kb on + strand, within SGL_RS05470 at 772.008 kb on + strand, within SGL_RS05470 at 772.085 kb on + strand, within SGL_RS05470 at 772.202 kb on - strand, within SGL_RS05470 at 772.290 kb on + strand, within SGL_RS05470 at 772.300 kb on + strand, within SGL_RS05470 at 772.399 kb on + strand, within SGL_RS05470
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 770,113 - SGL_RS05455 0.86 +0.5 770,391 + SGL_RS05460 0.17 +0.8 771,248 - SGL_RS05465 0.58 -1.8 771,265 - SGL_RS05465 0.61 -0.2 771,332 - SGL_RS05465 0.73 -0.4 771,351 + SGL_RS05465 0.76 -4.0 771,351 + SGL_RS05465 0.76 -2.7 771,426 - -3.1 771,547 - +0.9 771,566 + +2.6 771,574 - -0.0 771,597 + +2.3 771,648 + +0.6 771,663 + +0.1 771,710 - -3.2 771,769 + SGL_RS05470 0.14 +0.8 771,769 + SGL_RS05470 0.14 -2.0 771,831 + SGL_RS05470 0.21 -1.2 772,008 + SGL_RS05470 0.41 -2.4 772,085 + SGL_RS05470 0.50 -1.2 772,202 - SGL_RS05470 0.63 -3.0 772,290 + SGL_RS05470 0.73 -2.2 772,300 + SGL_RS05470 0.75 -3.8 772,399 + SGL_RS05470 0.86 -2.3
Or see this region's nucleotide sequence