Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05260 and SGL_RS05265 are separated by 186 nucleotides SGL_RS05265 and SGL_RS05270 are separated by 236 nucleotides SGL_RS05270 and SGL_RS05280 are separated by 73 nucleotides SGL_RS05280 and SGL_RS05285 are separated by 226 nucleotides
SGL_RS05260: SGL_RS05260 - hypothetical protein, at 728,660 to 729,484
_RS05260
SGL_RS05265: SGL_RS05265 - hypothetical protein, at 729,671 to 729,871
_RS05265
SGL_RS05270: SGL_RS05270 - serine/threonine-protein kinase, at 730,108 to 731,674
_RS05270
SGL_RS05280: SGL_RS05280 - hypothetical protein, at 731,748 to 732,191
_RS05280
SGL_RS05285: SGL_RS05285 - aspartoacylase, at 732,418 to 733,284
_RS05285
Position (kb) 730
731
732 Strain fitness (log2 ratio) -1
0
1
2
3 at 729.197 kb on - strand, within SGL_RS05260 at 729.291 kb on - strand, within SGL_RS05260 at 729.425 kb on + strand at 729.425 kb on + strand at 729.432 kb on + strand at 729.780 kb on - strand, within SGL_RS05265 at 729.800 kb on - strand, within SGL_RS05265 at 729.952 kb on + strand at 730.072 kb on + strand at 730.072 kb on + strand at 730.072 kb on + strand at 730.080 kb on - strand at 730.148 kb on - strand at 730.221 kb on + strand at 730.375 kb on - strand, within SGL_RS05270 at 730.402 kb on - strand, within SGL_RS05270 at 730.490 kb on - strand, within SGL_RS05270 at 730.876 kb on + strand, within SGL_RS05270 at 730.951 kb on - strand, within SGL_RS05270 at 730.953 kb on + strand, within SGL_RS05270 at 730.953 kb on + strand, within SGL_RS05270 at 731.036 kb on - strand, within SGL_RS05270 at 731.064 kb on - strand, within SGL_RS05270 at 731.073 kb on + strand, within SGL_RS05270 at 731.082 kb on - strand, within SGL_RS05270 at 731.287 kb on + strand, within SGL_RS05270 at 731.583 kb on + strand at 731.607 kb on - strand at 731.774 kb on + strand at 731.774 kb on + strand at 731.842 kb on - strand, within SGL_RS05280 at 731.933 kb on + strand, within SGL_RS05280 at 731.941 kb on - strand, within SGL_RS05280 at 731.943 kb on + strand, within SGL_RS05280 at 732.023 kb on - strand, within SGL_RS05280 at 732.023 kb on - strand, within SGL_RS05280 at 732.031 kb on - strand, within SGL_RS05280 at 732.119 kb on - strand, within SGL_RS05280 at 732.155 kb on - strand at 732.166 kb on + strand at 732.232 kb on - strand at 732.234 kb on - strand at 732.617 kb on + strand, within SGL_RS05285
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 729,197 - SGL_RS05260 0.65 -1.0 729,291 - SGL_RS05260 0.76 -0.8 729,425 + -0.7 729,425 + -0.4 729,432 + -0.5 729,780 - SGL_RS05265 0.54 +0.5 729,800 - SGL_RS05265 0.64 +0.4 729,952 + +0.3 730,072 + -0.1 730,072 + +0.1 730,072 + +0.3 730,080 - +0.2 730,148 - -0.7 730,221 + +2.4 730,375 - SGL_RS05270 0.17 +1.8 730,402 - SGL_RS05270 0.19 +1.8 730,490 - SGL_RS05270 0.24 +1.4 730,876 + SGL_RS05270 0.49 +1.6 730,951 - SGL_RS05270 0.54 +1.4 730,953 + SGL_RS05270 0.54 +1.2 730,953 + SGL_RS05270 0.54 +1.7 731,036 - SGL_RS05270 0.59 +3.4 731,064 - SGL_RS05270 0.61 +2.2 731,073 + SGL_RS05270 0.62 +1.4 731,082 - SGL_RS05270 0.62 +1.8 731,287 + SGL_RS05270 0.75 +1.7 731,583 + +2.2 731,607 - +1.7 731,774 + -0.5 731,774 + -0.5 731,842 - SGL_RS05280 0.21 +0.1 731,933 + SGL_RS05280 0.42 -0.1 731,941 - SGL_RS05280 0.43 -0.1 731,943 + SGL_RS05280 0.44 +0.5 732,023 - SGL_RS05280 0.62 +0.1 732,023 - SGL_RS05280 0.62 +0.1 732,031 - SGL_RS05280 0.64 +0.1 732,119 - SGL_RS05280 0.84 -0.1 732,155 - +0.1 732,166 + +0.4 732,232 - -0.3 732,234 - -0.1 732,617 + SGL_RS05285 0.23 +0.2
Or see this region's nucleotide sequence