Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05210 and SGL_RS05215 are separated by 190 nucleotides SGL_RS05215 and SGL_RS05220 are separated by 31 nucleotides SGL_RS05220 and SGL_RS05225 are separated by 90 nucleotides
SGL_RS05210: SGL_RS05210 - SLBB domain-containing protein, at 719,733 to 721,238
_RS05210
SGL_RS05215: SGL_RS05215 - TIGR02450 family Trp-rich protein, at 721,429 to 721,692
_RS05215
SGL_RS05220: SGL_RS05220 - DUF1995 family protein, at 721,724 to 722,479
_RS05220
SGL_RS05225: SGL_RS05225 - serine--tRNA ligase, at 722,570 to 723,862
_RS05225
Position (kb) 721
722
723 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 720.832 kb on - strand, within SGL_RS05210 at 721.017 kb on - strand, within SGL_RS05210 at 721.037 kb on + strand, within SGL_RS05210 at 721.056 kb on - strand, within SGL_RS05210 at 721.112 kb on - strand at 721.166 kb on + strand at 721.166 kb on + strand at 721.201 kb on - strand at 721.329 kb on - strand at 721.471 kb on - strand, within SGL_RS05215 at 721.652 kb on - strand, within SGL_RS05215 at 721.722 kb on - strand at 721.751 kb on + strand at 721.945 kb on + strand, within SGL_RS05220 at 722.241 kb on + strand, within SGL_RS05220 at 722.249 kb on - strand, within SGL_RS05220 at 722.435 kb on + strand at 722.568 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 720,832 - SGL_RS05210 0.73 -3.2 721,017 - SGL_RS05210 0.85 -3.0 721,037 + SGL_RS05210 0.87 -2.0 721,056 - SGL_RS05210 0.88 -2.5 721,112 - -1.3 721,166 + -3.5 721,166 + -3.6 721,201 - -2.8 721,329 - +0.1 721,471 - SGL_RS05215 0.16 +0.2 721,652 - SGL_RS05215 0.84 -0.1 721,722 - +0.6 721,751 + +1.1 721,945 + SGL_RS05220 0.29 +1.3 722,241 + SGL_RS05220 0.68 +1.0 722,249 - SGL_RS05220 0.69 +1.0 722,435 + -0.0 722,568 - +2.2
Or see this region's nucleotide sequence