Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05110 and SGL_RS05115 are separated by 15 nucleotides SGL_RS05115 and SGL_RS05120 are separated by 264 nucleotides SGL_RS05120 and SGL_RS05125 are separated by 7 nucleotides
SGL_RS05110: SGL_RS05110 - ATP-dependent zinc metalloprotease FtsH1, at 695,975 to 697,972
_RS05110
SGL_RS05115: SGL_RS05115 - hypothetical protein, at 697,988 to 698,458
_RS05115
SGL_RS05120: SGL_RS05120 - ferrous iron transport protein A, at 698,723 to 698,962
_RS05120
SGL_RS05125: SGL_RS05125 - ferrous iron transport protein B, at 698,970 to 700,814
_RS05125
Position (kb) 697
698
699 Strain fitness (log2 ratio) -1
0
1
2 at 697.937 kb on - strand at 697.981 kb on - strand at 698.091 kb on - strand, within SGL_RS05115 at 698.106 kb on + strand, within SGL_RS05115 at 698.485 kb on + strand at 698.574 kb on + strand at 698.789 kb on + strand, within SGL_RS05120 at 698.797 kb on - strand, within SGL_RS05120 at 698.797 kb on - strand, within SGL_RS05120 at 698.965 kb on - strand at 698.970 kb on - strand at 698.985 kb on - strand at 699.055 kb on - strand at 699.149 kb on + strand at 699.201 kb on + strand, within SGL_RS05125 at 699.380 kb on + strand, within SGL_RS05125 at 699.388 kb on - strand, within SGL_RS05125 at 699.388 kb on - strand, within SGL_RS05125 at 699.421 kb on + strand, within SGL_RS05125 at 699.441 kb on - strand, within SGL_RS05125
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 697,937 - +0.7 697,981 - +0.0 698,091 - SGL_RS05115 0.22 +2.2 698,106 + SGL_RS05115 0.25 +2.2 698,485 + -0.1 698,574 + +0.3 698,789 + SGL_RS05120 0.28 +0.2 698,797 - SGL_RS05120 0.31 +0.3 698,797 - SGL_RS05120 0.31 +0.5 698,965 - +0.5 698,970 - +0.1 698,985 - -1.1 699,055 - -0.0 699,149 + +1.3 699,201 + SGL_RS05125 0.13 -0.2 699,380 + SGL_RS05125 0.22 +0.1 699,388 - SGL_RS05125 0.23 +0.8 699,388 - SGL_RS05125 0.23 -0.1 699,421 + SGL_RS05125 0.24 +0.3 699,441 - SGL_RS05125 0.26 -0.1
Or see this region's nucleotide sequence