Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04775 and SGL_RS04780 are separated by 90 nucleotides SGL_RS04780 and SGL_RS04785 are separated by 149 nucleotides SGL_RS04785 and SGL_RS04790 are separated by 52 nucleotides
SGL_RS04775: SGL_RS04775 - alpha/beta hydrolase, at 619,618 to 620,469
_RS04775
SGL_RS04780: SGL_RS04780 - alpha/beta fold hydrolase, at 620,560 to 621,468
_RS04780
SGL_RS04785: SGL_RS04785 - CPP1-like family protein, at 621,618 to 622,247
_RS04785
SGL_RS04790: SGL_RS04790 - penicillin-binding protein 2, at 622,300 to 624,123
_RS04790
Position (kb) 620
621
622 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 619.561 kb on - strand at 619.657 kb on + strand at 619.961 kb on - strand, within SGL_RS04775 at 619.976 kb on - strand, within SGL_RS04775 at 620.115 kb on + strand, within SGL_RS04775 at 620.369 kb on - strand, within SGL_RS04775 at 620.567 kb on + strand at 620.608 kb on + strand at 620.710 kb on - strand, within SGL_RS04780 at 620.775 kb on - strand, within SGL_RS04780 at 620.970 kb on + strand, within SGL_RS04780 at 620.970 kb on + strand, within SGL_RS04780 at 621.010 kb on + strand, within SGL_RS04780 at 621.018 kb on - strand, within SGL_RS04780 at 621.105 kb on + strand, within SGL_RS04780 at 621.105 kb on + strand, within SGL_RS04780 at 621.149 kb on + strand, within SGL_RS04780 at 621.257 kb on - strand, within SGL_RS04780 at 621.263 kb on + strand, within SGL_RS04780 at 621.292 kb on + strand, within SGL_RS04780 at 621.292 kb on - strand, within SGL_RS04780 at 621.354 kb on + strand, within SGL_RS04780 at 621.362 kb on + strand, within SGL_RS04780 at 621.411 kb on - strand at 621.449 kb on + strand at 621.457 kb on - strand at 621.457 kb on - strand at 621.457 kb on - strand at 621.462 kb on + strand at 621.462 kb on + strand at 621.481 kb on - strand at 621.483 kb on - strand at 621.531 kb on + strand at 621.531 kb on - strand at 621.608 kb on + strand at 621.711 kb on + strand, within SGL_RS04785 at 621.916 kb on - strand, within SGL_RS04785 at 622.013 kb on - strand, within SGL_RS04785 at 622.174 kb on + strand, within SGL_RS04785 at 622.308 kb on - strand at 622.317 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 619,561 - -0.7 619,657 + -5.3 619,961 - SGL_RS04775 0.40 -2.9 619,976 - SGL_RS04775 0.42 -5.4 620,115 + SGL_RS04775 0.58 -6.2 620,369 - SGL_RS04775 0.88 -4.4 620,567 + -0.3 620,608 + -1.3 620,710 - SGL_RS04780 0.17 -1.3 620,775 - SGL_RS04780 0.24 +1.0 620,970 + SGL_RS04780 0.45 -0.3 620,970 + SGL_RS04780 0.45 -0.3 621,010 + SGL_RS04780 0.50 +0.2 621,018 - SGL_RS04780 0.50 -0.7 621,105 + SGL_RS04780 0.60 +0.4 621,105 + SGL_RS04780 0.60 -0.2 621,149 + SGL_RS04780 0.65 -0.6 621,257 - SGL_RS04780 0.77 +0.1 621,263 + SGL_RS04780 0.77 +0.3 621,292 + SGL_RS04780 0.81 -0.2 621,292 - SGL_RS04780 0.81 -0.4 621,354 + SGL_RS04780 0.87 -0.1 621,362 + SGL_RS04780 0.88 -0.4 621,411 - -0.2 621,449 + +0.6 621,457 - -0.2 621,457 - -0.3 621,457 - +0.7 621,462 + -0.4 621,462 + +0.5 621,481 - +0.1 621,483 - -0.4 621,531 + -1.0 621,531 - +0.4 621,608 + +1.5 621,711 + SGL_RS04785 0.15 +0.3 621,916 - SGL_RS04785 0.47 +0.7 622,013 - SGL_RS04785 0.63 +0.1 622,174 + SGL_RS04785 0.88 +0.4 622,308 - -1.7 622,317 + -1.4
Or see this region's nucleotide sequence