Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04765 and SGL_RS04770 are separated by 520 nucleotides SGL_RS04770 and SGL_RS04775 are separated by 88 nucleotides SGL_RS04775 and SGL_RS04780 are separated by 90 nucleotides
SGL_RS04765: SGL_RS04765 - TM2 domain-containing protein, at 618,311 to 618,694
_RS04765
SGL_RS04770: SGL_RS04770 - DUF1825 family protein, at 619,215 to 619,529
_RS04770
SGL_RS04775: SGL_RS04775 - alpha/beta hydrolase, at 619,618 to 620,469
_RS04775
SGL_RS04780: SGL_RS04780 - alpha/beta fold hydrolase, at 620,560 to 621,468
_RS04780
Position (kb) 619
620
621 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 618.624 kb on + strand, within SGL_RS04765 at 618.695 kb on - strand at 618.802 kb on + strand at 618.835 kb on - strand at 619.128 kb on + strand at 619.206 kb on - strand at 619.414 kb on - strand, within SGL_RS04770 at 619.521 kb on + strand at 619.561 kb on - strand at 619.657 kb on + strand at 619.961 kb on - strand, within SGL_RS04775 at 619.976 kb on - strand, within SGL_RS04775 at 620.115 kb on + strand, within SGL_RS04775 at 620.369 kb on - strand, within SGL_RS04775 at 620.567 kb on + strand at 620.608 kb on + strand at 620.710 kb on - strand, within SGL_RS04780 at 620.775 kb on - strand, within SGL_RS04780 at 620.970 kb on + strand, within SGL_RS04780 at 620.970 kb on + strand, within SGL_RS04780 at 621.010 kb on + strand, within SGL_RS04780 at 621.018 kb on - strand, within SGL_RS04780 at 621.105 kb on + strand, within SGL_RS04780 at 621.105 kb on + strand, within SGL_RS04780 at 621.149 kb on + strand, within SGL_RS04780 at 621.257 kb on - strand, within SGL_RS04780 at 621.263 kb on + strand, within SGL_RS04780 at 621.292 kb on + strand, within SGL_RS04780 at 621.292 kb on - strand, within SGL_RS04780 at 621.354 kb on + strand, within SGL_RS04780 at 621.362 kb on + strand, within SGL_RS04780 at 621.411 kb on - strand at 621.449 kb on + strand at 621.457 kb on - strand at 621.457 kb on - strand at 621.457 kb on - strand at 621.462 kb on + strand at 621.462 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 618,624 + SGL_RS04765 0.82 -0.8 618,695 - -3.3 618,802 + -0.3 618,835 - +0.3 619,128 + +0.3 619,206 - -0.2 619,414 - SGL_RS04770 0.63 +0.1 619,521 + -0.6 619,561 - -0.7 619,657 + -5.3 619,961 - SGL_RS04775 0.40 -2.9 619,976 - SGL_RS04775 0.42 -5.4 620,115 + SGL_RS04775 0.58 -6.2 620,369 - SGL_RS04775 0.88 -4.4 620,567 + -0.3 620,608 + -1.3 620,710 - SGL_RS04780 0.17 -1.3 620,775 - SGL_RS04780 0.24 +1.0 620,970 + SGL_RS04780 0.45 -0.3 620,970 + SGL_RS04780 0.45 -0.3 621,010 + SGL_RS04780 0.50 +0.2 621,018 - SGL_RS04780 0.50 -0.7 621,105 + SGL_RS04780 0.60 +0.4 621,105 + SGL_RS04780 0.60 -0.2 621,149 + SGL_RS04780 0.65 -0.6 621,257 - SGL_RS04780 0.77 +0.1 621,263 + SGL_RS04780 0.77 +0.3 621,292 + SGL_RS04780 0.81 -0.2 621,292 - SGL_RS04780 0.81 -0.4 621,354 + SGL_RS04780 0.87 -0.1 621,362 + SGL_RS04780 0.88 -0.4 621,411 - -0.2 621,449 + +0.6 621,457 - -0.2 621,457 - -0.3 621,457 - +0.7 621,462 + -0.4 621,462 + +0.5
Or see this region's nucleotide sequence