Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04600 and SGL_RS04605 are separated by 75 nucleotides SGL_RS04605 and SGL_RS04610 are separated by 3 nucleotides SGL_RS04610 and SGL_RS04615 are separated by 64 nucleotides SGL_RS04615 and SGL_RS04620 are separated by 106 nucleotides
SGL_RS04600: SGL_RS04600 - hypothetical protein, at 581,518 to 582,162
_RS04600
SGL_RS04605: SGL_RS04605 - hypothetical protein, at 582,238 to 582,477
_RS04605
SGL_RS04610: SGL_RS04610 - Uma2 family endonuclease, at 582,481 to 583,092
_RS04610
SGL_RS04615: SGL_RS04615 - Fur family transcriptional regulator, at 583,157 to 583,573
_RS04615
SGL_RS04620: SGL_RS04620 - zinc ABC transporter substrate-binding protein, at 583,680 to 584,696
_RS04620
Position (kb) 583
584 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 582.173 kb on + strand at 582.180 kb on + strand at 582.367 kb on + strand, within SGL_RS04605 at 582.392 kb on + strand, within SGL_RS04605 at 582.400 kb on - strand, within SGL_RS04605 at 582.584 kb on + strand, within SGL_RS04610 at 582.686 kb on + strand, within SGL_RS04610 at 582.767 kb on + strand, within SGL_RS04610 at 583.115 kb on + strand at 583.115 kb on + strand at 583.149 kb on + strand at 583.269 kb on - strand, within SGL_RS04615 at 583.272 kb on + strand, within SGL_RS04615 at 583.306 kb on - strand, within SGL_RS04615 at 583.335 kb on - strand, within SGL_RS04615 at 583.400 kb on + strand, within SGL_RS04615 at 583.416 kb on + strand, within SGL_RS04615 at 583.521 kb on - strand, within SGL_RS04615 at 583.521 kb on - strand, within SGL_RS04615 at 583.527 kb on + strand, within SGL_RS04615 at 583.662 kb on - strand at 583.672 kb on - strand at 583.672 kb on - strand at 583.672 kb on - strand at 583.672 kb on - strand at 583.748 kb on - strand at 583.750 kb on + strand at 583.778 kb on - strand at 583.900 kb on + strand, within SGL_RS04620 at 583.930 kb on + strand, within SGL_RS04620 at 583.959 kb on + strand, within SGL_RS04620 at 583.967 kb on - strand, within SGL_RS04620 at 583.981 kb on + strand, within SGL_RS04620 at 584.031 kb on - strand, within SGL_RS04620 at 584.041 kb on - strand, within SGL_RS04620 at 584.042 kb on - strand, within SGL_RS04620 at 584.077 kb on - strand, within SGL_RS04620 at 584.191 kb on - strand, within SGL_RS04620 at 584.216 kb on + strand, within SGL_RS04620 at 584.227 kb on + strand, within SGL_RS04620 at 584.384 kb on - strand, within SGL_RS04620 at 584.453 kb on - strand, within SGL_RS04620 at 584.569 kb on + strand, within SGL_RS04620
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 582,173 + +0.3 582,180 + +0.5 582,367 + SGL_RS04605 0.54 +1.0 582,392 + SGL_RS04605 0.64 -0.1 582,400 - SGL_RS04605 0.68 -1.3 582,584 + SGL_RS04610 0.17 -0.5 582,686 + SGL_RS04610 0.33 -0.4 582,767 + SGL_RS04610 0.47 -0.3 583,115 + +0.2 583,115 + +0.1 583,149 + +0.5 583,269 - SGL_RS04615 0.27 -2.4 583,272 + SGL_RS04615 0.28 -2.8 583,306 - SGL_RS04615 0.36 -2.1 583,335 - SGL_RS04615 0.43 -2.8 583,400 + SGL_RS04615 0.58 -3.6 583,416 + SGL_RS04615 0.62 -3.0 583,521 - SGL_RS04615 0.87 -2.9 583,521 - SGL_RS04615 0.87 -2.3 583,527 + SGL_RS04615 0.89 -2.2 583,662 - +0.2 583,672 - -1.8 583,672 - -1.4 583,672 - -1.1 583,672 - -0.5 583,748 - -0.1 583,750 + -0.1 583,778 - -0.5 583,900 + SGL_RS04620 0.22 -0.4 583,930 + SGL_RS04620 0.25 +0.1 583,959 + SGL_RS04620 0.27 -0.3 583,967 - SGL_RS04620 0.28 +0.4 583,981 + SGL_RS04620 0.30 +0.2 584,031 - SGL_RS04620 0.35 +0.8 584,041 - SGL_RS04620 0.35 -1.0 584,042 - SGL_RS04620 0.36 +0.2 584,077 - SGL_RS04620 0.39 -0.7 584,191 - SGL_RS04620 0.50 -0.2 584,216 + SGL_RS04620 0.53 -0.3 584,227 + SGL_RS04620 0.54 +0.5 584,384 - SGL_RS04620 0.69 -1.8 584,453 - SGL_RS04620 0.76 -0.0 584,569 + SGL_RS04620 0.87 -0.5
Or see this region's nucleotide sequence