Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04435 and SGL_RS04440 are separated by 197 nucleotides SGL_RS04440 and SGL_RS04445 are separated by 94 nucleotides SGL_RS04445 and SGL_RS04450 are separated by 269 nucleotides SGL_RS04450 and SGL_RS04455 are separated by 180 nucleotides
SGL_RS04435: SGL_RS04435 - FAD-dependent thymidylate synthase, at 552,220 to 552,867
_RS04435
SGL_RS04440: SGL_RS04440 - photosystem II reaction center protein K, at 553,065 to 553,202
_RS04440
SGL_RS04445: SGL_RS04445 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 553,297 to 554,019
_RS04445
SGL_RS04450: SGL_RS04450 - BolA family transcriptional regulator, at 554,289 to 554,546
_RS04450
SGL_RS04455: SGL_RS04455 - Grx4 family monothiol glutaredoxin, at 554,727 to 555,050
_RS04455
Position (kb) 553
554
555 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 553.340 kb on + strand at 553.348 kb on - strand at 553.370 kb on - strand, within SGL_RS04445 at 553.484 kb on + strand, within SGL_RS04445 at 553.492 kb on - strand, within SGL_RS04445 at 553.508 kb on + strand, within SGL_RS04445 at 553.516 kb on - strand, within SGL_RS04445 at 553.520 kb on + strand, within SGL_RS04445 at 553.576 kb on + strand, within SGL_RS04445 at 553.732 kb on + strand, within SGL_RS04445 at 553.732 kb on + strand, within SGL_RS04445 at 553.751 kb on - strand, within SGL_RS04445 at 553.810 kb on - strand, within SGL_RS04445 at 553.846 kb on + strand, within SGL_RS04445 at 553.849 kb on - strand, within SGL_RS04445 at 553.999 kb on + strand at 554.157 kb on - strand at 554.161 kb on + strand at 554.199 kb on + strand at 554.225 kb on - strand at 554.256 kb on + strand at 554.264 kb on - strand at 554.304 kb on - strand at 554.368 kb on - strand, within SGL_RS04450 at 554.386 kb on - strand, within SGL_RS04450 at 554.417 kb on + strand, within SGL_RS04450 at 554.432 kb on + strand, within SGL_RS04450 at 554.524 kb on - strand at 554.524 kb on - strand at 554.715 kb on + strand at 554.797 kb on + strand, within SGL_RS04455 at 554.948 kb on + strand, within SGL_RS04455 at 554.985 kb on - strand, within SGL_RS04455 at 554.997 kb on - strand, within SGL_RS04455
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 553,340 + +0.6 553,348 - -1.6 553,370 - SGL_RS04445 0.10 +4.6 553,484 + SGL_RS04445 0.26 +5.2 553,492 - SGL_RS04445 0.27 +4.8 553,508 + SGL_RS04445 0.29 +5.0 553,516 - SGL_RS04445 0.30 +5.1 553,520 + SGL_RS04445 0.31 +5.1 553,576 + SGL_RS04445 0.39 +4.6 553,732 + SGL_RS04445 0.60 +4.8 553,732 + SGL_RS04445 0.60 +5.0 553,751 - SGL_RS04445 0.63 +4.9 553,810 - SGL_RS04445 0.71 +5.2 553,846 + SGL_RS04445 0.76 +5.3 553,849 - SGL_RS04445 0.76 +5.4 553,999 + +4.8 554,157 - +1.4 554,161 + -1.8 554,199 + +0.9 554,225 - +0.3 554,256 + +0.7 554,264 - -0.1 554,304 - -0.3 554,368 - SGL_RS04450 0.31 -0.3 554,386 - SGL_RS04450 0.38 +0.3 554,417 + SGL_RS04450 0.50 +0.2 554,432 + SGL_RS04450 0.55 +0.7 554,524 - +0.1 554,524 - +0.2 554,715 + +0.5 554,797 + SGL_RS04455 0.22 +0.1 554,948 + SGL_RS04455 0.68 +0.0 554,985 - SGL_RS04455 0.80 +0.3 554,997 - SGL_RS04455 0.83 +0.3
Or see this region's nucleotide sequence