Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04310 and SGL_RS04315 are separated by 162 nucleotides SGL_RS04315 and SGL_RS04320 are separated by 22 nucleotides SGL_RS04320 and SGL_RS04325 are separated by 288 nucleotides
SGL_RS04310: SGL_RS04310 - MlaD family protein, at 521,483 to 522,853
_RS04310
SGL_RS04315: SGL_RS04315 - ABC transporter ATP-binding protein, at 523,016 to 523,798
_RS04315
SGL_RS04320: SGL_RS04320 - hypothetical protein, at 523,821 to 524,093
_RS04320
SGL_RS04325: SGL_RS04325 - CHAT domain-containing protein, at 524,382 to 529,595
_RS04325
Position (kb) 523
524
525 Strain fitness (log2 ratio) -1
0
1
2 at 522.846 kb on - strand at 522.852 kb on - strand at 522.873 kb on - strand at 522.970 kb on - strand at 523.194 kb on - strand, within SGL_RS04315 at 523.267 kb on - strand, within SGL_RS04315 at 523.350 kb on + strand, within SGL_RS04315 at 523.397 kb on - strand, within SGL_RS04315 at 523.579 kb on + strand, within SGL_RS04315 at 523.615 kb on + strand, within SGL_RS04315 at 523.723 kb on + strand at 523.723 kb on + strand at 523.731 kb on - strand at 523.757 kb on + strand at 523.762 kb on + strand at 523.783 kb on - strand at 523.918 kb on - strand, within SGL_RS04320 at 523.941 kb on + strand, within SGL_RS04320 at 524.022 kb on + strand, within SGL_RS04320 at 524.317 kb on - strand at 524.459 kb on - strand at 524.480 kb on + strand at 524.599 kb on - strand at 524.715 kb on - strand at 524.850 kb on + strand at 524.937 kb on + strand, within SGL_RS04325
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 522,846 - +0.2 522,852 - +1.5 522,873 - +0.1 522,970 - -0.2 523,194 - SGL_RS04315 0.23 +0.1 523,267 - SGL_RS04315 0.32 +1.2 523,350 + SGL_RS04315 0.43 +0.6 523,397 - SGL_RS04315 0.49 +1.8 523,579 + SGL_RS04315 0.72 +0.7 523,615 + SGL_RS04315 0.77 +1.2 523,723 + +1.0 523,723 + +0.9 523,731 - +0.7 523,757 + +0.5 523,762 + +0.6 523,783 - +2.5 523,918 - SGL_RS04320 0.36 +1.0 523,941 + SGL_RS04320 0.44 +0.7 524,022 + SGL_RS04320 0.74 +1.4 524,317 - +0.3 524,459 - -0.1 524,480 + +0.8 524,599 - +0.3 524,715 - +0.6 524,850 + +0.4 524,937 + SGL_RS04325 0.11 +0.5
Or see this region's nucleotide sequence