Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03840 and SGL_RS03845 are separated by 157 nucleotides SGL_RS03845 and SGL_RS03850 are separated by 110 nucleotides SGL_RS03850 and SGL_RS03855 are separated by 35 nucleotides
SGL_RS03840: SGL_RS03840 - adenylyl-sulfate kinase, at 414,871 to 415,404
_RS03840
SGL_RS03845: SGL_RS03845 - MotA/TolQ/ExbB proton channel family protein, at 415,562 to 416,245
_RS03845
SGL_RS03850: SGL_RS03850 - biopolymer transporter ExbD, at 416,356 to 417,165
_RS03850
SGL_RS03855: SGL_RS03855 - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 417,201 to 418,541
_RS03855
Position (kb) 416
417
418 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 415.418 kb on - strand at 415.418 kb on - strand at 415.726 kb on + strand, within SGL_RS03845 at 415.838 kb on + strand, within SGL_RS03845 at 415.910 kb on + strand, within SGL_RS03845 at 415.913 kb on - strand, within SGL_RS03845 at 415.939 kb on - strand, within SGL_RS03845 at 416.033 kb on + strand, within SGL_RS03845 at 416.038 kb on + strand, within SGL_RS03845 at 416.103 kb on + strand, within SGL_RS03845 at 416.430 kb on - strand at 416.897 kb on + strand, within SGL_RS03850 at 416.900 kb on - strand, within SGL_RS03850 at 416.902 kb on + strand, within SGL_RS03850 at 416.935 kb on - strand, within SGL_RS03850 at 417.016 kb on + strand, within SGL_RS03850 at 417.063 kb on - strand, within SGL_RS03850 at 417.160 kb on - strand at 417.273 kb on + strand at 417.356 kb on - strand, within SGL_RS03855 at 417.474 kb on - strand, within SGL_RS03855 at 417.474 kb on - strand, within SGL_RS03855 at 417.536 kb on - strand, within SGL_RS03855 at 417.545 kb on - strand, within SGL_RS03855 at 417.595 kb on - strand, within SGL_RS03855 at 417.753 kb on - strand, within SGL_RS03855 at 417.777 kb on - strand, within SGL_RS03855 at 417.777 kb on - strand, within SGL_RS03855 at 417.780 kb on + strand, within SGL_RS03855 at 417.780 kb on + strand, within SGL_RS03855 at 417.788 kb on - strand, within SGL_RS03855 at 417.795 kb on - strand, within SGL_RS03855 at 417.838 kb on + strand, within SGL_RS03855 at 417.838 kb on + strand, within SGL_RS03855 at 417.838 kb on + strand, within SGL_RS03855 at 417.846 kb on - strand, within SGL_RS03855 at 417.906 kb on + strand, within SGL_RS03855 at 417.914 kb on - strand, within SGL_RS03855 at 417.941 kb on - strand, within SGL_RS03855 at 418.001 kb on + strand, within SGL_RS03855
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 415,418 - -1.9 415,418 - -1.5 415,726 + SGL_RS03845 0.24 -1.5 415,838 + SGL_RS03845 0.40 -1.8 415,910 + SGL_RS03845 0.51 -2.4 415,913 - SGL_RS03845 0.51 -1.5 415,939 - SGL_RS03845 0.55 -2.3 416,033 + SGL_RS03845 0.69 -1.9 416,038 + SGL_RS03845 0.70 -1.4 416,103 + SGL_RS03845 0.79 -2.4 416,430 - -2.0 416,897 + SGL_RS03850 0.67 -1.6 416,900 - SGL_RS03850 0.67 -1.6 416,902 + SGL_RS03850 0.67 -0.8 416,935 - SGL_RS03850 0.71 -3.5 417,016 + SGL_RS03850 0.81 -2.2 417,063 - SGL_RS03850 0.87 -2.4 417,160 - -1.1 417,273 + -0.4 417,356 - SGL_RS03855 0.12 -1.0 417,474 - SGL_RS03855 0.20 -0.7 417,474 - SGL_RS03855 0.20 -1.0 417,536 - SGL_RS03855 0.25 -0.3 417,545 - SGL_RS03855 0.26 -0.7 417,595 - SGL_RS03855 0.29 -1.0 417,753 - SGL_RS03855 0.41 -1.2 417,777 - SGL_RS03855 0.43 -0.8 417,777 - SGL_RS03855 0.43 -1.0 417,780 + SGL_RS03855 0.43 -1.1 417,780 + SGL_RS03855 0.43 +0.0 417,788 - SGL_RS03855 0.44 -1.1 417,795 - SGL_RS03855 0.44 +0.1 417,838 + SGL_RS03855 0.48 -2.2 417,838 + SGL_RS03855 0.48 -0.5 417,838 + SGL_RS03855 0.48 -0.5 417,846 - SGL_RS03855 0.48 +0.2 417,906 + SGL_RS03855 0.53 +1.5 417,914 - SGL_RS03855 0.53 +0.2 417,941 - SGL_RS03855 0.55 -0.6 418,001 + SGL_RS03855 0.60 -1.1
Or see this region's nucleotide sequence