Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03765 and SGL_RS03770 are separated by 58 nucleotides SGL_RS03770 and SGL_RS03775 are separated by 16 nucleotides SGL_RS03775 and SGL_RS03780 are separated by 146 nucleotides
SGL_RS03765: SGL_RS03765 - bestrophin family protein, at 390,905 to 391,840
_RS03765
SGL_RS03770: SGL_RS03770 - succinate--CoA ligase subunit beta, at 391,899 to 393,104
_RS03770
SGL_RS03775: SGL_RS03775 - membrane protein, at 393,121 to 393,849
_RS03775
SGL_RS03780: SGL_RS03780 - flotillin family protein, at 393,996 to 396,017
_RS03780
Position (kb) 391
392
393
394 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 390.962 kb on + strand at 390.965 kb on + strand at 390.970 kb on - strand at 391.103 kb on - strand, within SGL_RS03765 at 391.175 kb on + strand, within SGL_RS03765 at 391.183 kb on - strand, within SGL_RS03765 at 391.227 kb on + strand, within SGL_RS03765 at 391.542 kb on + strand, within SGL_RS03765 at 391.550 kb on - strand, within SGL_RS03765 at 391.659 kb on - strand, within SGL_RS03765 at 391.721 kb on + strand, within SGL_RS03765 at 391.729 kb on - strand, within SGL_RS03765 at 391.896 kb on + strand at 391.928 kb on - strand at 392.075 kb on - strand, within SGL_RS03770 at 392.142 kb on - strand, within SGL_RS03770 at 392.165 kb on - strand, within SGL_RS03770 at 392.186 kb on + strand, within SGL_RS03770 at 392.195 kb on - strand, within SGL_RS03770 at 392.283 kb on + strand, within SGL_RS03770 at 392.351 kb on + strand, within SGL_RS03770 at 392.383 kb on + strand, within SGL_RS03770 at 392.430 kb on + strand, within SGL_RS03770 at 392.539 kb on + strand, within SGL_RS03770 at 392.584 kb on - strand, within SGL_RS03770 at 392.672 kb on + strand, within SGL_RS03770 at 392.771 kb on - strand, within SGL_RS03770 at 392.781 kb on - strand, within SGL_RS03770 at 392.781 kb on - strand, within SGL_RS03770 at 392.977 kb on - strand, within SGL_RS03770 at 392.992 kb on - strand at 393.017 kb on - strand at 393.099 kb on + strand at 393.112 kb on + strand at 393.305 kb on + strand, within SGL_RS03775 at 393.351 kb on + strand, within SGL_RS03775 at 393.371 kb on + strand, within SGL_RS03775 at 393.372 kb on + strand, within SGL_RS03775 at 393.372 kb on + strand, within SGL_RS03775 at 393.380 kb on - strand, within SGL_RS03775 at 393.578 kb on - strand, within SGL_RS03775 at 393.658 kb on + strand, within SGL_RS03775 at 393.658 kb on + strand, within SGL_RS03775 at 393.666 kb on - strand, within SGL_RS03775 at 393.772 kb on - strand, within SGL_RS03775 at 393.849 kb on - strand at 393.851 kb on + strand at 393.871 kb on - strand at 393.894 kb on - strand at 394.083 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 390,962 + -0.4 390,965 + -1.8 390,970 - +0.4 391,103 - SGL_RS03765 0.21 +0.4 391,175 + SGL_RS03765 0.29 +0.2 391,183 - SGL_RS03765 0.30 +0.4 391,227 + SGL_RS03765 0.34 +0.3 391,542 + SGL_RS03765 0.68 +0.8 391,550 - SGL_RS03765 0.69 +0.5 391,659 - SGL_RS03765 0.81 -0.2 391,721 + SGL_RS03765 0.87 +0.5 391,729 - SGL_RS03765 0.88 +0.5 391,896 + +0.8 391,928 - +0.2 392,075 - SGL_RS03770 0.15 +0.2 392,142 - SGL_RS03770 0.20 -0.9 392,165 - SGL_RS03770 0.22 -0.4 392,186 + SGL_RS03770 0.24 -0.2 392,195 - SGL_RS03770 0.25 -0.8 392,283 + SGL_RS03770 0.32 -0.5 392,351 + SGL_RS03770 0.37 -0.1 392,383 + SGL_RS03770 0.40 -0.6 392,430 + SGL_RS03770 0.44 -0.5 392,539 + SGL_RS03770 0.53 -2.5 392,584 - SGL_RS03770 0.57 -0.4 392,672 + SGL_RS03770 0.64 -0.1 392,771 - SGL_RS03770 0.72 -0.1 392,781 - SGL_RS03770 0.73 -0.5 392,781 - SGL_RS03770 0.73 +0.8 392,977 - SGL_RS03770 0.89 -0.6 392,992 - -1.2 393,017 - -0.9 393,099 + +0.3 393,112 + +0.4 393,305 + SGL_RS03775 0.25 +2.4 393,351 + SGL_RS03775 0.32 +1.7 393,371 + SGL_RS03775 0.34 +2.0 393,372 + SGL_RS03775 0.34 +1.8 393,372 + SGL_RS03775 0.34 +1.7 393,380 - SGL_RS03775 0.36 +1.4 393,578 - SGL_RS03775 0.63 +2.1 393,658 + SGL_RS03775 0.74 +2.9 393,658 + SGL_RS03775 0.74 +3.1 393,666 - SGL_RS03775 0.75 +3.6 393,772 - SGL_RS03775 0.89 +2.1 393,849 - +0.6 393,851 + +2.5 393,871 - +2.5 393,894 - +2.9 394,083 - +0.2
Or see this region's nucleotide sequence