Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03635 and SGL_RS03640 overlap by 4 nucleotides SGL_RS03640 and SGL_RS03645 are separated by 301 nucleotides SGL_RS03645 and SGL_RS03650 are separated by 63 nucleotides
SGL_RS03635: SGL_RS03635 - glycosyltransferase family 4 protein, at 370,285 to 371,490
_RS03635
SGL_RS03640: SGL_RS03640 - UDP-glucose 4-epimerase GalE, at 371,487 to 372,509
_RS03640
SGL_RS03645: SGL_RS03645 - FkbM family methyltransferase, at 372,811 to 373,167
_RS03645
SGL_RS03650: SGL_RS03650 - FkbM family methyltransferase, at 373,231 to 373,644
_RS03650
Position (kb) 371
372
373 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 370.627 kb on - strand, within SGL_RS03635 at 370.984 kb on - strand, within SGL_RS03635 at 371.381 kb on + strand at 371.389 kb on - strand at 371.389 kb on - strand at 371.393 kb on + strand at 371.503 kb on - strand at 371.571 kb on + strand at 371.582 kb on - strand at 371.642 kb on - strand, within SGL_RS03640 at 372.012 kb on - strand, within SGL_RS03640 at 372.196 kb on - strand, within SGL_RS03640 at 372.366 kb on + strand, within SGL_RS03640 at 372.366 kb on + strand, within SGL_RS03640 at 372.376 kb on + strand, within SGL_RS03640 at 372.612 kb on + strand at 373.147 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 370,627 - SGL_RS03635 0.28 -1.4 370,984 - SGL_RS03635 0.58 -0.8 371,381 + -1.9 371,389 - -1.7 371,389 - -2.0 371,393 + -2.1 371,503 - -1.9 371,571 + -2.5 371,582 - -1.1 371,642 - SGL_RS03640 0.15 -1.6 372,012 - SGL_RS03640 0.51 -2.2 372,196 - SGL_RS03640 0.69 -2.2 372,366 + SGL_RS03640 0.86 -3.2 372,366 + SGL_RS03640 0.86 -1.0 372,376 + SGL_RS03640 0.87 -2.1 372,612 + -1.1 373,147 - +0.3
Or see this region's nucleotide sequence