Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03550 and SGL_RS03555 are separated by 18 nucleotides SGL_RS03555 and SGL_RS03560 overlap by 4 nucleotides SGL_RS03560 and SGL_RS03565 are separated by 34 nucleotides SGL_RS03565 and SGL_RS03570 are separated by 50 nucleotides
SGL_RS03550: SGL_RS03550 - CDP-glucose 4,6-dehydratase, at 354,323 to 355,405
_RS03550
SGL_RS03555: SGL_RS03555 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 355,424 to 355,993
_RS03555
SGL_RS03560: SGL_RS03560 - class I SAM-dependent methyltransferase, at 355,990 to 357,210
_RS03560
SGL_RS03565: SGL_RS03565 - DUF29 domain-containing protein, at 357,245 to 357,697
_RS03565
SGL_RS03570: SGL_RS03570 - Uma2 family endonuclease, at 357,748 to 358,608
_RS03570
Position (kb) 355
356
357
358 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 355.078 kb on - strand, within SGL_RS03550 at 355.092 kb on - strand, within SGL_RS03550 at 355.217 kb on + strand, within SGL_RS03550 at 355.308 kb on + strand at 355.375 kb on + strand at 355.382 kb on - strand at 355.584 kb on + strand, within SGL_RS03555 at 355.584 kb on + strand, within SGL_RS03555 at 355.606 kb on - strand, within SGL_RS03555 at 355.740 kb on + strand, within SGL_RS03555 at 355.748 kb on - strand, within SGL_RS03555 at 355.765 kb on - strand, within SGL_RS03555 at 355.789 kb on - strand, within SGL_RS03555 at 355.831 kb on + strand, within SGL_RS03555 at 355.859 kb on - strand, within SGL_RS03555 at 355.909 kb on - strand, within SGL_RS03555 at 355.920 kb on - strand, within SGL_RS03555 at 356.014 kb on - strand at 356.210 kb on + strand, within SGL_RS03560 at 356.211 kb on + strand, within SGL_RS03560 at 356.535 kb on + strand, within SGL_RS03560 at 356.563 kb on - strand, within SGL_RS03560 at 356.676 kb on - strand, within SGL_RS03560 at 356.742 kb on + strand, within SGL_RS03560 at 356.820 kb on - strand, within SGL_RS03560 at 356.949 kb on + strand, within SGL_RS03560 at 356.959 kb on - strand, within SGL_RS03560 at 357.035 kb on - strand, within SGL_RS03560 at 357.433 kb on - strand, within SGL_RS03565 at 357.474 kb on + strand, within SGL_RS03565 at 357.487 kb on - strand, within SGL_RS03565
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 355,078 - SGL_RS03550 0.70 -5.3 355,092 - SGL_RS03550 0.71 -5.8 355,217 + SGL_RS03550 0.83 -6.4 355,308 + -7.0 355,375 + +0.3 355,382 - +0.6 355,584 + SGL_RS03555 0.28 -3.6 355,584 + SGL_RS03555 0.28 -5.4 355,606 - SGL_RS03555 0.32 -5.6 355,740 + SGL_RS03555 0.55 -5.4 355,748 - SGL_RS03555 0.57 -6.0 355,765 - SGL_RS03555 0.60 -4.4 355,789 - SGL_RS03555 0.64 -5.8 355,831 + SGL_RS03555 0.71 -7.0 355,859 - SGL_RS03555 0.76 -5.4 355,909 - SGL_RS03555 0.85 -3.2 355,920 - SGL_RS03555 0.87 -4.5 356,014 - -6.4 356,210 + SGL_RS03560 0.18 -5.7 356,211 + SGL_RS03560 0.18 -2.9 356,535 + SGL_RS03560 0.45 -2.4 356,563 - SGL_RS03560 0.47 -6.0 356,676 - SGL_RS03560 0.56 -6.5 356,742 + SGL_RS03560 0.62 -5.8 356,820 - SGL_RS03560 0.68 -5.4 356,949 + SGL_RS03560 0.79 -6.2 356,959 - SGL_RS03560 0.79 -6.5 357,035 - SGL_RS03560 0.86 -5.6 357,433 - SGL_RS03565 0.42 +0.1 357,474 + SGL_RS03565 0.51 +0.3 357,487 - SGL_RS03565 0.53 -0.0
Or see this region's nucleotide sequence