Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03540 and SGL_RS03545 overlap by 4 nucleotides SGL_RS03545 and SGL_RS03550 are separated by 0 nucleotides SGL_RS03550 and SGL_RS03555 are separated by 18 nucleotides SGL_RS03555 and SGL_RS03560 overlap by 4 nucleotides
SGL_RS03540: SGL_RS03540 - ABC transporter ATP-binding protein, at 352,263 to 353,555
_RS03540
SGL_RS03545: SGL_RS03545 - glucose-1-phosphate cytidylyltransferase, at 353,552 to 354,322
_RS03545
SGL_RS03550: SGL_RS03550 - CDP-glucose 4,6-dehydratase, at 354,323 to 355,405
_RS03550
SGL_RS03555: SGL_RS03555 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 355,424 to 355,993
_RS03555
SGL_RS03560: SGL_RS03560 - class I SAM-dependent methyltransferase, at 355,990 to 357,210
_RS03560
Position (kb) 354
355
356 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 353.481 kb on + strand at 353.486 kb on + strand at 353.820 kb on - strand, within SGL_RS03545 at 354.014 kb on + strand, within SGL_RS03545 at 354.213 kb on - strand, within SGL_RS03545 at 354.409 kb on - strand at 354.459 kb on + strand, within SGL_RS03550 at 354.468 kb on - strand, within SGL_RS03550 at 354.504 kb on + strand, within SGL_RS03550 at 354.590 kb on + strand, within SGL_RS03550 at 354.602 kb on - strand, within SGL_RS03550 at 354.614 kb on - strand, within SGL_RS03550 at 354.615 kb on + strand, within SGL_RS03550 at 354.667 kb on - strand, within SGL_RS03550 at 354.760 kb on + strand, within SGL_RS03550 at 354.790 kb on + strand, within SGL_RS03550 at 354.869 kb on + strand, within SGL_RS03550 at 354.877 kb on - strand, within SGL_RS03550 at 355.078 kb on - strand, within SGL_RS03550 at 355.092 kb on - strand, within SGL_RS03550 at 355.217 kb on + strand, within SGL_RS03550 at 355.308 kb on + strand at 355.375 kb on + strand at 355.382 kb on - strand at 355.584 kb on + strand, within SGL_RS03555 at 355.584 kb on + strand, within SGL_RS03555 at 355.606 kb on - strand, within SGL_RS03555 at 355.740 kb on + strand, within SGL_RS03555 at 355.748 kb on - strand, within SGL_RS03555 at 355.765 kb on - strand, within SGL_RS03555 at 355.789 kb on - strand, within SGL_RS03555 at 355.831 kb on + strand, within SGL_RS03555 at 355.859 kb on - strand, within SGL_RS03555 at 355.909 kb on - strand, within SGL_RS03555 at 355.920 kb on - strand, within SGL_RS03555 at 356.014 kb on - strand at 356.210 kb on + strand, within SGL_RS03560 at 356.211 kb on + strand, within SGL_RS03560
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 353,481 + -3.7 353,486 + -4.7 353,820 - SGL_RS03545 0.35 -3.2 354,014 + SGL_RS03545 0.60 -5.8 354,213 - SGL_RS03545 0.86 -6.2 354,409 - -4.9 354,459 + SGL_RS03550 0.13 -5.4 354,468 - SGL_RS03550 0.13 -2.0 354,504 + SGL_RS03550 0.17 -5.6 354,590 + SGL_RS03550 0.25 -2.9 354,602 - SGL_RS03550 0.26 -6.7 354,614 - SGL_RS03550 0.27 -0.7 354,615 + SGL_RS03550 0.27 -5.3 354,667 - SGL_RS03550 0.32 -7.5 354,760 + SGL_RS03550 0.40 -6.4 354,790 + SGL_RS03550 0.43 -6.3 354,869 + SGL_RS03550 0.50 -6.5 354,877 - SGL_RS03550 0.51 -6.0 355,078 - SGL_RS03550 0.70 -5.3 355,092 - SGL_RS03550 0.71 -5.8 355,217 + SGL_RS03550 0.83 -6.4 355,308 + -7.0 355,375 + +0.3 355,382 - +0.6 355,584 + SGL_RS03555 0.28 -3.6 355,584 + SGL_RS03555 0.28 -5.4 355,606 - SGL_RS03555 0.32 -5.6 355,740 + SGL_RS03555 0.55 -5.4 355,748 - SGL_RS03555 0.57 -6.0 355,765 - SGL_RS03555 0.60 -4.4 355,789 - SGL_RS03555 0.64 -5.8 355,831 + SGL_RS03555 0.71 -7.0 355,859 - SGL_RS03555 0.76 -5.4 355,909 - SGL_RS03555 0.85 -3.2 355,920 - SGL_RS03555 0.87 -4.5 356,014 - -6.4 356,210 + SGL_RS03560 0.18 -5.7 356,211 + SGL_RS03560 0.18 -2.9
Or see this region's nucleotide sequence