Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03485 and SGL_RS03490 are separated by 303 nucleotides SGL_RS03490 and SGL_RS03495 are separated by 23 nucleotides SGL_RS03495 and SGL_RS03500 are separated by 99 nucleotides SGL_RS03500 and SGL_RS03505 are separated by 65 nucleotides
SGL_RS03485: SGL_RS03485 - translation initiation factor IF-3, at 343,026 to 343,559
_RS03485
SGL_RS03490: SGL_RS03490 - YaaW family protein, at 343,863 to 344,720
_RS03490
SGL_RS03495: SGL_RS03495 - metal-binding protein, at 344,744 to 345,298
_RS03495
SGL_RS03500: SGL_RS03500 - tRNA-Val, at 345,398 to 345,470
_RS03500
SGL_RS03505: SGL_RS03505 - exopolysaccharide transport family protein, at 345,536 to 347,806
_RS03505
Position (kb) 343
344
345 Strain fitness (log2 ratio) -1
0
1 at 343.562 kb on - strand at 344.079 kb on - strand, within SGL_RS03490 at 344.095 kb on - strand, within SGL_RS03490 at 344.254 kb on - strand, within SGL_RS03490 at 344.273 kb on - strand, within SGL_RS03490 at 344.385 kb on + strand, within SGL_RS03490 at 344.597 kb on - strand, within SGL_RS03490 at 344.613 kb on + strand, within SGL_RS03490 at 344.684 kb on - strand at 344.842 kb on + strand, within SGL_RS03495 at 344.850 kb on - strand, within SGL_RS03495 at 344.913 kb on - strand, within SGL_RS03495 at 344.965 kb on + strand, within SGL_RS03495 at 345.178 kb on + strand, within SGL_RS03495 at 345.265 kb on + strand at 345.305 kb on + strand at 345.605 kb on + strand at 345.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 343,562 - +0.1 344,079 - SGL_RS03490 0.25 +0.8 344,095 - SGL_RS03490 0.27 +1.1 344,254 - SGL_RS03490 0.46 +1.4 344,273 - SGL_RS03490 0.48 +1.4 344,385 + SGL_RS03490 0.61 +0.6 344,597 - SGL_RS03490 0.86 +0.5 344,613 + SGL_RS03490 0.87 +1.1 344,684 - +0.9 344,842 + SGL_RS03495 0.18 -0.4 344,850 - SGL_RS03495 0.19 +1.5 344,913 - SGL_RS03495 0.30 +0.1 344,965 + SGL_RS03495 0.40 -0.3 345,178 + SGL_RS03495 0.78 +0.5 345,265 + +1.3 345,305 + -0.2 345,605 + -0.1 345,605 - -0.4
Or see this region's nucleotide sequence