Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03440 and SGL_RS03445 are separated by 17 nucleotides SGL_RS03445 and SGL_RS03450 are separated by 164 nucleotides SGL_RS03450 and SGL_RS03455 are separated by 100 nucleotides SGL_RS03455 and SGL_RS03460 are separated by 171 nucleotides
SGL_RS03440: SGL_RS03440 - PAM68 family protein, at 335,819 to 336,295
_RS03440
SGL_RS03445: SGL_RS03445 - 30S ribosomal protein S15, at 336,313 to 336,582
_RS03445
SGL_RS03450: SGL_RS03450 - permease, at 336,747 to 337,760
_RS03450
SGL_RS03455: SGL_RS03455 - tetratricopeptide repeat protein, at 337,861 to 338,331
_RS03455
SGL_RS03460: SGL_RS03460 - hypothetical protein, at 338,503 to 339,063
_RS03460
Position (kb)
336
337
338 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 335.837 kb on - strand at 335.837 kb on - strand at 335.842 kb on - strand at 335.906 kb on + strand, within SGL_RS03440 at 335.914 kb on - strand, within SGL_RS03440 at 335.914 kb on - strand, within SGL_RS03440 at 335.965 kb on - strand, within SGL_RS03440 at 335.992 kb on + strand, within SGL_RS03440 at 336.101 kb on - strand, within SGL_RS03440 at 336.106 kb on - strand, within SGL_RS03440 at 336.704 kb on + strand at 336.842 kb on + strand at 336.850 kb on - strand, within SGL_RS03450 at 337.389 kb on - strand, within SGL_RS03450 at 337.410 kb on - strand, within SGL_RS03450 at 337.415 kb on - strand, within SGL_RS03450 at 337.931 kb on + strand, within SGL_RS03455 at 337.933 kb on + strand, within SGL_RS03455 at 338.162 kb on - strand, within SGL_RS03455 at 338.162 kb on - strand, within SGL_RS03455 at 338.162 kb on - strand, within SGL_RS03455 at 338.225 kb on + strand, within SGL_RS03455 at 338.225 kb on + strand, within SGL_RS03455 at 338.239 kb on - strand, within SGL_RS03455 at 338.428 kb on + strand at 338.618 kb on - strand, within SGL_RS03460 at 338.679 kb on + strand, within SGL_RS03460 at 338.724 kb on + strand, within SGL_RS03460
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 335,837 - +0.5 335,837 - +0.6 335,842 - +1.3 335,906 + SGL_RS03440 0.18 +1.7 335,914 - SGL_RS03440 0.20 +2.0 335,914 - SGL_RS03440 0.20 +2.2 335,965 - SGL_RS03440 0.31 +1.9 335,992 + SGL_RS03440 0.36 +2.9 336,101 - SGL_RS03440 0.59 +2.5 336,106 - SGL_RS03440 0.60 +2.3 336,704 + +1.3 336,842 + -1.0 336,850 - SGL_RS03450 0.10 -3.3 337,389 - SGL_RS03450 0.63 -4.5 337,410 - SGL_RS03450 0.65 -4.1 337,415 - SGL_RS03450 0.66 -1.8 337,931 + SGL_RS03455 0.15 -0.1 337,933 + SGL_RS03455 0.15 -1.1 338,162 - SGL_RS03455 0.64 -0.4 338,162 - SGL_RS03455 0.64 -0.8 338,162 - SGL_RS03455 0.64 -0.7 338,225 + SGL_RS03455 0.77 -1.0 338,225 + SGL_RS03455 0.77 -0.0 338,239 - SGL_RS03455 0.80 -0.4 338,428 + +0.4 338,618 - SGL_RS03460 0.20 -0.4 338,679 + SGL_RS03460 0.31 +0.2 338,724 + SGL_RS03460 0.39 +0.4
Or see this region's nucleotide sequence