Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03410 and SGL_RS03415 are separated by 40 nucleotides SGL_RS03415 and SGL_RS03420 are separated by 33 nucleotides SGL_RS03420 and SGL_RS03425 are separated by 83 nucleotides
SGL_RS03410: SGL_RS03410 - LL-diaminopimelate aminotransferase, at 328,006 to 329,181
_RS03410
SGL_RS03415: SGL_RS03415 - SH3 domain-containing protein, at 329,222 to 329,521
_RS03415
SGL_RS03420: SGL_RS03420 - UbiD family decarboxylase, at 329,555 to 331,060
_RS03420
SGL_RS03425: SGL_RS03425 - precorrin-3B C(17)-methyltransferase, at 331,144 to 333,027
_RS03425
Position (kb) 329
330
331
332 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 328.598 kb on + strand, within SGL_RS03410 at 328.603 kb on + strand, within SGL_RS03410 at 328.690 kb on + strand, within SGL_RS03410 at 328.698 kb on - strand, within SGL_RS03410 at 328.712 kb on + strand, within SGL_RS03410 at 328.720 kb on - strand, within SGL_RS03410 at 328.801 kb on + strand, within SGL_RS03410 at 328.822 kb on - strand, within SGL_RS03410 at 329.019 kb on - strand, within SGL_RS03410 at 329.053 kb on + strand, within SGL_RS03410 at 329.097 kb on - strand at 329.100 kb on + strand at 329.627 kb on + strand at 329.651 kb on + strand at 329.663 kb on + strand at 329.674 kb on - strand at 329.868 kb on - strand, within SGL_RS03420 at 330.016 kb on - strand, within SGL_RS03420 at 330.068 kb on + strand, within SGL_RS03420 at 330.330 kb on - strand, within SGL_RS03420 at 330.330 kb on - strand, within SGL_RS03420 at 330.547 kb on + strand, within SGL_RS03420 at 330.555 kb on - strand, within SGL_RS03420 at 330.783 kb on - strand, within SGL_RS03420 at 330.870 kb on - strand, within SGL_RS03420 at 330.900 kb on - strand, within SGL_RS03420 at 330.946 kb on - strand at 330.988 kb on + strand at 331.058 kb on - strand at 331.063 kb on - strand at 331.075 kb on - strand at 331.077 kb on + strand at 331.620 kb on - strand, within SGL_RS03425 at 331.870 kb on + strand, within SGL_RS03425 at 331.980 kb on - strand, within SGL_RS03425
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 328,598 + SGL_RS03410 0.50 -0.3 328,603 + SGL_RS03410 0.51 -0.6 328,690 + SGL_RS03410 0.58 +0.4 328,698 - SGL_RS03410 0.59 -0.3 328,712 + SGL_RS03410 0.60 -0.4 328,720 - SGL_RS03410 0.61 -0.8 328,801 + SGL_RS03410 0.68 -0.5 328,822 - SGL_RS03410 0.69 -2.4 329,019 - SGL_RS03410 0.86 -0.2 329,053 + SGL_RS03410 0.89 -0.2 329,097 - +0.0 329,100 + -0.0 329,627 + -2.8 329,651 + -5.2 329,663 + -3.5 329,674 - -3.5 329,868 - SGL_RS03420 0.21 -3.5 330,016 - SGL_RS03420 0.31 -4.8 330,068 + SGL_RS03420 0.34 -0.2 330,330 - SGL_RS03420 0.51 -4.5 330,330 - SGL_RS03420 0.51 -3.9 330,547 + SGL_RS03420 0.66 -4.4 330,555 - SGL_RS03420 0.66 -4.0 330,783 - SGL_RS03420 0.82 -6.3 330,870 - SGL_RS03420 0.87 -1.9 330,900 - SGL_RS03420 0.89 -3.2 330,946 - -5.5 330,988 + -2.2 331,058 - -0.4 331,063 - -1.9 331,075 - -3.9 331,077 + -3.1 331,620 - SGL_RS03425 0.25 -2.4 331,870 + SGL_RS03425 0.39 -2.4 331,980 - SGL_RS03425 0.44 -3.0
Or see this region's nucleotide sequence