Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03235 and SGL_RS03240 are separated by 56 nucleotides SGL_RS03240 and SGL_RS03245 are separated by 451 nucleotides SGL_RS03245 and SGL_RS03250 are separated by 81 nucleotides SGL_RS03250 and SGL_RS19555 are separated by 10 nucleotides
SGL_RS03235: SGL_RS03235 - C-3',4' desaturase CrtD, at 289,322 to 290,845
_RS03235
SGL_RS03240: SGL_RS03240 - YggT family protein, at 290,902 to 291,174
_RS03240
SGL_RS03245: SGL_RS03245 - Fe(3+) ABC transporter substrate-binding protein, at 291,626 to 292,708
_RS03245
SGL_RS03250: SGL_RS03250 - tRNA (guanosine(37)-N1)-methyltransferase TrmD, at 292,790 to 293,485
_RS03250
SGL_RS19555: SGL_RS19555 - hypothetical protein, at 293,496 to 293,669
_RS19555
Position (kb) 291
292
293 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 290.645 kb on + strand, within SGL_RS03235 at 290.670 kb on + strand, within SGL_RS03235 at 290.691 kb on + strand, within SGL_RS03235 at 290.691 kb on + strand, within SGL_RS03235 at 290.824 kb on - strand at 290.939 kb on + strand, within SGL_RS03240 at 290.940 kb on + strand, within SGL_RS03240 at 291.003 kb on - strand, within SGL_RS03240 at 291.062 kb on + strand, within SGL_RS03240 at 291.062 kb on + strand, within SGL_RS03240 at 291.070 kb on - strand, within SGL_RS03240 at 291.070 kb on - strand, within SGL_RS03240 at 291.070 kb on - strand, within SGL_RS03240 at 291.716 kb on - strand at 291.728 kb on - strand at 291.752 kb on - strand, within SGL_RS03245 at 291.771 kb on - strand, within SGL_RS03245 at 291.820 kb on - strand, within SGL_RS03245 at 291.891 kb on - strand, within SGL_RS03245 at 291.898 kb on + strand, within SGL_RS03245 at 291.898 kb on + strand, within SGL_RS03245 at 292.224 kb on + strand, within SGL_RS03245 at 292.230 kb on + strand, within SGL_RS03245 at 292.242 kb on - strand, within SGL_RS03245 at 292.248 kb on - strand, within SGL_RS03245 at 292.282 kb on - strand, within SGL_RS03245 at 292.307 kb on + strand, within SGL_RS03245 at 292.358 kb on - strand, within SGL_RS03245 at 292.371 kb on + strand, within SGL_RS03245 at 292.447 kb on + strand, within SGL_RS03245 at 292.471 kb on + strand, within SGL_RS03245 at 292.487 kb on + strand, within SGL_RS03245 at 292.489 kb on - strand, within SGL_RS03245 at 292.509 kb on + strand, within SGL_RS03245 at 292.659 kb on - strand at 292.661 kb on + strand at 292.669 kb on - strand at 292.669 kb on - strand at 292.781 kb on + strand at 292.895 kb on - strand, within SGL_RS03250 at 292.923 kb on + strand, within SGL_RS03250 at 293.154 kb on + strand, within SGL_RS03250 at 293.167 kb on - strand, within SGL_RS03250 at 293.227 kb on - strand, within SGL_RS03250 at 293.227 kb on - strand, within SGL_RS03250 at 293.330 kb on - strand, within SGL_RS03250 at 293.412 kb on + strand, within SGL_RS03250 at 293.585 kb on - strand, within SGL_RS19555 at 293.607 kb on - strand, within SGL_RS19555 at 293.698 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 290,645 + SGL_RS03235 0.87 +0.9 290,670 + SGL_RS03235 0.88 +1.1 290,691 + SGL_RS03235 0.90 +0.9 290,691 + SGL_RS03235 0.90 +0.9 290,824 - +0.3 290,939 + SGL_RS03240 0.14 +0.3 290,940 + SGL_RS03240 0.14 +0.7 291,003 - SGL_RS03240 0.37 +0.2 291,062 + SGL_RS03240 0.59 +0.4 291,062 + SGL_RS03240 0.59 +0.4 291,070 - SGL_RS03240 0.62 -0.8 291,070 - SGL_RS03240 0.62 +0.1 291,070 - SGL_RS03240 0.62 +0.3 291,716 - +3.0 291,728 - +3.4 291,752 - SGL_RS03245 0.12 +2.9 291,771 - SGL_RS03245 0.13 +2.9 291,820 - SGL_RS03245 0.18 +3.3 291,891 - SGL_RS03245 0.24 +3.3 291,898 + SGL_RS03245 0.25 +2.9 291,898 + SGL_RS03245 0.25 +3.2 292,224 + SGL_RS03245 0.55 +4.1 292,230 + SGL_RS03245 0.56 +3.2 292,242 - SGL_RS03245 0.57 +3.9 292,248 - SGL_RS03245 0.57 +3.1 292,282 - SGL_RS03245 0.61 +3.5 292,307 + SGL_RS03245 0.63 +4.6 292,358 - SGL_RS03245 0.68 +3.3 292,371 + SGL_RS03245 0.69 +3.0 292,447 + SGL_RS03245 0.76 +3.7 292,471 + SGL_RS03245 0.78 +3.4 292,487 + SGL_RS03245 0.80 +3.6 292,489 - SGL_RS03245 0.80 +3.1 292,509 + SGL_RS03245 0.82 +3.3 292,659 - +3.1 292,661 + +3.5 292,669 - +3.7 292,669 - +3.1 292,781 + +1.2 292,895 - SGL_RS03250 0.15 -3.0 292,923 + SGL_RS03250 0.19 -2.9 293,154 + SGL_RS03250 0.52 -1.5 293,167 - SGL_RS03250 0.54 -4.3 293,227 - SGL_RS03250 0.63 -2.4 293,227 - SGL_RS03250 0.63 -1.3 293,330 - SGL_RS03250 0.78 -0.6 293,412 + SGL_RS03250 0.89 -3.3 293,585 - SGL_RS19555 0.51 +0.3 293,607 - SGL_RS19555 0.64 +0.4 293,698 + -0.3
Or see this region's nucleotide sequence