Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03055 and SGL_RS03060 are separated by 146 nucleotides SGL_RS03060 and SGL_RS03065 are separated by 51 nucleotides SGL_RS03065 and SGL_RS03070 are separated by 196 nucleotides SGL_RS03070 and SGL_RS03075 are separated by 57 nucleotides
SGL_RS03055: SGL_RS03055 - protoporphyrinogen oxidase HemJ, at 256,647 to 257,279
_RS03055
SGL_RS03060: SGL_RS03060 - phosphoadenosine phosphosulfate reductase, at 257,426 to 258,175
_RS03060
SGL_RS03065: SGL_RS03065 - heat-inducible transcriptional repressor HrcA, at 258,227 to 259,363
_RS03065
SGL_RS03070: SGL_RS03070 - tRNA-Ser, at 259,560 to 259,644
_RS03070
SGL_RS03075: SGL_RS03075 - shikimate kinase, at 259,702 to 260,271
_RS03075
Position (kb) 258
259
260 Strain fitness (log2 ratio) -2
-1
0
1
2 at 257.240 kb on + strand at 257.359 kb on - strand at 257.397 kb on - strand at 257.397 kb on - strand at 257.428 kb on + strand at 257.691 kb on + strand, within SGL_RS03060 at 257.699 kb on - strand, within SGL_RS03060 at 257.711 kb on + strand, within SGL_RS03060 at 257.866 kb on - strand, within SGL_RS03060 at 258.024 kb on - strand, within SGL_RS03060 at 258.052 kb on + strand, within SGL_RS03060 at 258.056 kb on + strand, within SGL_RS03060 at 258.297 kb on - strand at 258.335 kb on - strand at 258.619 kb on - strand, within SGL_RS03065 at 258.853 kb on + strand, within SGL_RS03065 at 259.099 kb on - strand, within SGL_RS03065 at 259.149 kb on + strand, within SGL_RS03065 at 259.177 kb on - strand, within SGL_RS03065 at 259.262 kb on - strand at 259.264 kb on + strand at 259.264 kb on + strand at 259.272 kb on - strand at 259.272 kb on - strand at 259.498 kb on - strand at 259.692 kb on - strand at 259.727 kb on + strand at 259.727 kb on + strand at 259.728 kb on - strand at 260.279 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 257,240 + -0.9 257,359 - +0.4 257,397 - +0.2 257,397 - +0.7 257,428 + +1.4 257,691 + SGL_RS03060 0.35 -2.3 257,699 - SGL_RS03060 0.36 -1.5 257,711 + SGL_RS03060 0.38 -1.8 257,866 - SGL_RS03060 0.59 -1.1 258,024 - SGL_RS03060 0.80 -1.6 258,052 + SGL_RS03060 0.83 -2.0 258,056 + SGL_RS03060 0.84 -1.2 258,297 - -0.3 258,335 - +1.2 258,619 - SGL_RS03065 0.34 +1.7 258,853 + SGL_RS03065 0.55 +1.6 259,099 - SGL_RS03065 0.77 +1.2 259,149 + SGL_RS03065 0.81 +1.7 259,177 - SGL_RS03065 0.84 +1.4 259,262 - +1.2 259,264 + +0.4 259,264 + +1.8 259,272 - +1.7 259,272 - +0.1 259,498 - +0.8 259,692 - -0.3 259,727 + +0.2 259,727 + +0.1 259,728 - +1.3 260,279 + +0.1
Or see this region's nucleotide sequence