Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03000 and SGL_RS03005 are separated by 74 nucleotides SGL_RS03005 and SGL_RS03010 are separated by 107 nucleotides SGL_RS03010 and SGL_RS03015 are separated by 97 nucleotides SGL_RS03015 and SGL_RS03020 are separated by 100 nucleotides
SGL_RS03000: SGL_RS03000 - spore maturation protein, at 244,160 to 244,708
_RS03000
SGL_RS03005: SGL_RS03005 - nucleoside recognition domain-containing protein, at 244,783 to 245,394
_RS03005
SGL_RS03010: SGL_RS03010 - 4-alpha-glucanotransferase, at 245,502 to 247,019
_RS03010
SGL_RS03015: SGL_RS03015 - VOC family protein, at 247,117 to 247,569
_RS03015
SGL_RS03020: SGL_RS03020 - thiamine-phosphate kinase, at 247,670 to 248,713
_RS03020
Position (kb) 245
246
247
248 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 244.540 kb on - strand, within SGL_RS03000 at 244.570 kb on - strand, within SGL_RS03000 at 245.163 kb on + strand, within SGL_RS03005 at 245.171 kb on - strand, within SGL_RS03005 at 245.406 kb on - strand at 245.426 kb on - strand at 245.468 kb on - strand at 245.601 kb on - strand at 245.620 kb on - strand at 245.779 kb on - strand, within SGL_RS03010 at 245.787 kb on - strand, within SGL_RS03010 at 245.833 kb on + strand, within SGL_RS03010 at 245.833 kb on + strand, within SGL_RS03010 at 245.841 kb on - strand, within SGL_RS03010 at 245.950 kb on + strand, within SGL_RS03010 at 246.097 kb on + strand, within SGL_RS03010 at 246.353 kb on - strand, within SGL_RS03010 at 246.353 kb on - strand, within SGL_RS03010 at 246.371 kb on - strand, within SGL_RS03010 at 246.560 kb on - strand, within SGL_RS03010 at 246.571 kb on + strand, within SGL_RS03010 at 246.579 kb on - strand, within SGL_RS03010 at 246.671 kb on - strand, within SGL_RS03010 at 246.722 kb on - strand, within SGL_RS03010 at 246.850 kb on - strand, within SGL_RS03010 at 247.065 kb on - strand at 247.123 kb on + strand at 247.188 kb on + strand, within SGL_RS03015 at 247.301 kb on + strand, within SGL_RS03015 at 247.371 kb on + strand, within SGL_RS03015 at 247.515 kb on - strand, within SGL_RS03015 at 247.565 kb on - strand at 247.674 kb on - strand at 247.713 kb on - strand at 247.958 kb on - strand, within SGL_RS03020
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 244,540 - SGL_RS03000 0.69 -0.3 244,570 - SGL_RS03000 0.75 -5.6 245,163 + SGL_RS03005 0.62 -3.8 245,171 - SGL_RS03005 0.63 -6.1 245,406 - -6.4 245,426 - -5.3 245,468 - -0.7 245,601 - +2.6 245,620 - +1.8 245,779 - SGL_RS03010 0.18 +1.3 245,787 - SGL_RS03010 0.19 +1.4 245,833 + SGL_RS03010 0.22 +2.4 245,833 + SGL_RS03010 0.22 +1.6 245,841 - SGL_RS03010 0.22 +1.0 245,950 + SGL_RS03010 0.30 +2.3 246,097 + SGL_RS03010 0.39 +1.7 246,353 - SGL_RS03010 0.56 +1.7 246,353 - SGL_RS03010 0.56 +1.5 246,371 - SGL_RS03010 0.57 +2.0 246,560 - SGL_RS03010 0.70 +2.1 246,571 + SGL_RS03010 0.70 +2.0 246,579 - SGL_RS03010 0.71 +1.5 246,671 - SGL_RS03010 0.77 +1.6 246,722 - SGL_RS03010 0.80 +1.8 246,850 - SGL_RS03010 0.89 +2.3 247,065 - +0.3 247,123 + -0.1 247,188 + SGL_RS03015 0.16 +0.3 247,301 + SGL_RS03015 0.41 -0.1 247,371 + SGL_RS03015 0.56 +0.2 247,515 - SGL_RS03015 0.88 -0.3 247,565 - +0.1 247,674 - -0.7 247,713 - +0.4 247,958 - SGL_RS03020 0.28 -2.1
Or see this region's nucleotide sequence