Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02505 and SGL_RS02510 are separated by 116 nucleotides SGL_RS02510 and SGL_RS02515 overlap by 1 nucleotides SGL_RS02515 and SGL_RS02520 are separated by 234 nucleotides SGL_RS02520 and SGL_RS02525 are separated by 10 nucleotides
SGL_RS02505: SGL_RS02505 - precorrin-4 C(11)-methyltransferase, at 141,648 to 142,442
_RS02505
SGL_RS02510: SGL_RS02510 - BlaI/MecI/CopY family transcriptional regulator, at 142,559 to 142,981
_RS02510
SGL_RS02515: SGL_RS02515 - M56 family metallopeptidase, at 142,981 to 143,826
_RS02515
SGL_RS02520: SGL_RS02520 - thioredoxin-dependent thiol peroxidase, at 144,061 to 144,543
_RS02520
SGL_RS02525: SGL_RS02525 - hypothetical protein, at 144,554 to 145,081
_RS02525
Position (kb) 142
143
144 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 142.158 kb on + strand, within SGL_RS02505 at 142.166 kb on - strand, within SGL_RS02505 at 142.251 kb on - strand, within SGL_RS02505 at 142.336 kb on + strand, within SGL_RS02505 at 142.420 kb on + strand at 142.455 kb on - strand at 142.526 kb on - strand at 142.526 kb on - strand at 142.545 kb on - strand at 142.602 kb on + strand, within SGL_RS02510 at 142.813 kb on + strand, within SGL_RS02510 at 142.894 kb on + strand, within SGL_RS02510 at 142.950 kb on + strand at 143.018 kb on + strand at 143.023 kb on + strand at 143.210 kb on + strand, within SGL_RS02515 at 143.343 kb on + strand, within SGL_RS02515 at 143.357 kb on + strand, within SGL_RS02515 at 143.419 kb on + strand, within SGL_RS02515 at 143.543 kb on + strand, within SGL_RS02515 at 143.664 kb on + strand, within SGL_RS02515 at 143.927 kb on + strand at 144.473 kb on - strand, within SGL_RS02520 at 144.531 kb on - strand at 144.693 kb on - strand, within SGL_RS02525 at 144.766 kb on + strand, within SGL_RS02525 at 144.821 kb on + strand, within SGL_RS02525
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 142,158 + SGL_RS02505 0.64 -1.9 142,166 - SGL_RS02505 0.65 +0.7 142,251 - SGL_RS02505 0.76 -2.6 142,336 + SGL_RS02505 0.87 -2.8 142,420 + -0.1 142,455 - +0.3 142,526 - +0.1 142,526 - -0.1 142,545 - -0.7 142,602 + SGL_RS02510 0.10 -0.9 142,813 + SGL_RS02510 0.60 -0.2 142,894 + SGL_RS02510 0.79 -1.7 142,950 + -0.5 143,018 + +2.1 143,023 + +0.8 143,210 + SGL_RS02515 0.27 +2.9 143,343 + SGL_RS02515 0.43 +1.2 143,357 + SGL_RS02515 0.44 +2.3 143,419 + SGL_RS02515 0.52 +2.9 143,543 + SGL_RS02515 0.66 +2.1 143,664 + SGL_RS02515 0.81 +2.3 143,927 + -0.9 144,473 - SGL_RS02520 0.85 -0.9 144,531 - +1.4 144,693 - SGL_RS02525 0.26 -0.5 144,766 + SGL_RS02525 0.40 +0.0 144,821 + SGL_RS02525 0.51 -1.1
Or see this region's nucleotide sequence