Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02305 and SGL_RS02310 are separated by 0 nucleotides SGL_RS02310 and SGL_RS02315 are separated by 112 nucleotides
SGL_RS02305: SGL_RS02305 - NAD-dependent malic enzyme, at 99,402 to 100,793
_RS02305
SGL_RS02310: SGL_RS02310 - cysteine synthase A, at 100,794 to 101,771
_RS02310
SGL_RS02315: SGL_RS02315 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 101,884 to 102,831
_RS02315
Position (kb) 100
101
102 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 99.895 kb on - strand, within SGL_RS02305 at 100.009 kb on + strand, within SGL_RS02305 at 100.118 kb on + strand, within SGL_RS02305 at 100.121 kb on - strand, within SGL_RS02305 at 100.208 kb on - strand, within SGL_RS02305 at 100.229 kb on - strand, within SGL_RS02305 at 100.354 kb on - strand, within SGL_RS02305 at 100.439 kb on + strand, within SGL_RS02305 at 100.467 kb on + strand, within SGL_RS02305 at 100.472 kb on + strand, within SGL_RS02305 at 100.480 kb on - strand, within SGL_RS02305 at 100.676 kb on - strand at 100.678 kb on + strand at 100.684 kb on - strand at 100.691 kb on - strand at 101.089 kb on + strand, within SGL_RS02310 at 101.130 kb on - strand, within SGL_RS02310 at 101.139 kb on - strand, within SGL_RS02310 at 101.149 kb on - strand, within SGL_RS02310 at 101.179 kb on + strand, within SGL_RS02310 at 101.179 kb on + strand, within SGL_RS02310 at 101.187 kb on - strand, within SGL_RS02310 at 101.212 kb on - strand, within SGL_RS02310 at 101.218 kb on - strand, within SGL_RS02310 at 101.220 kb on + strand, within SGL_RS02310 at 101.308 kb on + strand, within SGL_RS02310 at 101.358 kb on - strand, within SGL_RS02310 at 101.363 kb on - strand, within SGL_RS02310 at 101.363 kb on - strand, within SGL_RS02310 at 101.415 kb on - strand, within SGL_RS02310 at 101.428 kb on + strand, within SGL_RS02310 at 101.436 kb on - strand, within SGL_RS02310 at 101.436 kb on - strand, within SGL_RS02310 at 101.449 kb on + strand, within SGL_RS02310 at 101.505 kb on + strand, within SGL_RS02310 at 101.735 kb on + strand at 101.781 kb on + strand at 101.781 kb on + strand at 101.809 kb on - strand at 101.819 kb on - strand at 101.824 kb on - strand at 101.844 kb on - strand at 101.885 kb on - strand at 102.211 kb on + strand, within SGL_RS02315
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 99,895 - SGL_RS02305 0.35 -2.3 100,009 + SGL_RS02305 0.44 -5.2 100,118 + SGL_RS02305 0.51 -4.3 100,121 - SGL_RS02305 0.52 -5.1 100,208 - SGL_RS02305 0.58 -4.9 100,229 - SGL_RS02305 0.59 -2.5 100,354 - SGL_RS02305 0.68 -4.8 100,439 + SGL_RS02305 0.74 -3.9 100,467 + SGL_RS02305 0.77 -5.7 100,472 + SGL_RS02305 0.77 -3.5 100,480 - SGL_RS02305 0.77 -2.9 100,676 - -1.6 100,678 + -3.2 100,684 - -0.3 100,691 - -3.2 101,089 + SGL_RS02310 0.30 +1.8 101,130 - SGL_RS02310 0.34 +1.5 101,139 - SGL_RS02310 0.35 +0.7 101,149 - SGL_RS02310 0.36 +1.6 101,179 + SGL_RS02310 0.39 +1.3 101,179 + SGL_RS02310 0.39 +1.5 101,187 - SGL_RS02310 0.40 +1.1 101,212 - SGL_RS02310 0.43 +1.9 101,218 - SGL_RS02310 0.43 +1.2 101,220 + SGL_RS02310 0.44 +1.0 101,308 + SGL_RS02310 0.53 +1.4 101,358 - SGL_RS02310 0.58 +1.3 101,363 - SGL_RS02310 0.58 +1.6 101,363 - SGL_RS02310 0.58 +2.5 101,415 - SGL_RS02310 0.63 +1.2 101,428 + SGL_RS02310 0.65 +1.0 101,436 - SGL_RS02310 0.66 +1.6 101,436 - SGL_RS02310 0.66 +1.5 101,449 + SGL_RS02310 0.67 +1.5 101,505 + SGL_RS02310 0.73 +0.5 101,735 + +1.5 101,781 + +0.3 101,781 + +1.5 101,809 - +1.1 101,819 - +0.5 101,824 - +1.0 101,844 - -0.2 101,885 - +0.1 102,211 + SGL_RS02315 0.34 -2.6
Or see this region's nucleotide sequence