Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02295 and SGL_RS02300 are separated by 127 nucleotides SGL_RS02300 and SGL_RS02305 are separated by 202 nucleotides SGL_RS02305 and SGL_RS02310 are separated by 0 nucleotides
SGL_RS02295: SGL_RS02295 - polyribonucleotide nucleotidyltransferase, at 96,325 to 98,481
_RS02295
SGL_RS02300: SGL_RS02300 - signal peptidase I, at 98,609 to 99,199
_RS02300
SGL_RS02305: SGL_RS02305 - NAD-dependent malic enzyme, at 99,402 to 100,793
_RS02305
SGL_RS02310: SGL_RS02310 - cysteine synthase A, at 100,794 to 101,771
_RS02310
Position (kb) 99
100
101 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 98.547 kb on + strand at 98.550 kb on - strand at 98.633 kb on - strand at 98.638 kb on - strand at 98.648 kb on - strand at 98.795 kb on - strand, within SGL_RS02300 at 98.945 kb on + strand, within SGL_RS02300 at 99.143 kb on - strand at 99.230 kb on + strand at 99.272 kb on + strand at 99.306 kb on + strand at 99.405 kb on - strand at 99.415 kb on + strand at 99.433 kb on + strand at 99.533 kb on + strand at 99.552 kb on - strand, within SGL_RS02305 at 99.618 kb on + strand, within SGL_RS02305 at 99.632 kb on - strand, within SGL_RS02305 at 99.743 kb on + strand, within SGL_RS02305 at 99.748 kb on - strand, within SGL_RS02305 at 99.749 kb on - strand, within SGL_RS02305 at 99.895 kb on - strand, within SGL_RS02305 at 100.009 kb on + strand, within SGL_RS02305 at 100.118 kb on + strand, within SGL_RS02305 at 100.121 kb on - strand, within SGL_RS02305 at 100.208 kb on - strand, within SGL_RS02305 at 100.229 kb on - strand, within SGL_RS02305 at 100.354 kb on - strand, within SGL_RS02305 at 100.439 kb on + strand, within SGL_RS02305 at 100.467 kb on + strand, within SGL_RS02305 at 100.472 kb on + strand, within SGL_RS02305 at 100.480 kb on - strand, within SGL_RS02305 at 100.676 kb on - strand at 100.678 kb on + strand at 100.684 kb on - strand at 100.691 kb on - strand at 101.089 kb on + strand, within SGL_RS02310 at 101.130 kb on - strand, within SGL_RS02310 at 101.139 kb on - strand, within SGL_RS02310 at 101.149 kb on - strand, within SGL_RS02310 at 101.179 kb on + strand, within SGL_RS02310 at 101.179 kb on + strand, within SGL_RS02310 at 101.187 kb on - strand, within SGL_RS02310 at 101.212 kb on - strand, within SGL_RS02310 at 101.218 kb on - strand, within SGL_RS02310 at 101.220 kb on + strand, within SGL_RS02310 at 101.308 kb on + strand, within SGL_RS02310 at 101.358 kb on - strand, within SGL_RS02310 at 101.363 kb on - strand, within SGL_RS02310 at 101.363 kb on - strand, within SGL_RS02310 at 101.415 kb on - strand, within SGL_RS02310 at 101.428 kb on + strand, within SGL_RS02310 at 101.436 kb on - strand, within SGL_RS02310 at 101.436 kb on - strand, within SGL_RS02310 at 101.449 kb on + strand, within SGL_RS02310 at 101.505 kb on + strand, within SGL_RS02310 at 101.735 kb on + strand at 101.781 kb on + strand at 101.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 98,547 + -0.3 98,550 - -0.1 98,633 - -0.3 98,638 - +0.1 98,648 - -0.4 98,795 - SGL_RS02300 0.31 -1.3 98,945 + SGL_RS02300 0.57 -3.1 99,143 - +0.1 99,230 + +0.2 99,272 + -0.8 99,306 + +0.2 99,405 - -1.3 99,415 + -3.5 99,433 + -5.4 99,533 + -2.5 99,552 - SGL_RS02305 0.11 -3.5 99,618 + SGL_RS02305 0.16 -2.7 99,632 - SGL_RS02305 0.17 -2.6 99,743 + SGL_RS02305 0.24 -4.9 99,748 - SGL_RS02305 0.25 -3.5 99,749 - SGL_RS02305 0.25 -4.9 99,895 - SGL_RS02305 0.35 -2.3 100,009 + SGL_RS02305 0.44 -5.2 100,118 + SGL_RS02305 0.51 -4.3 100,121 - SGL_RS02305 0.52 -5.1 100,208 - SGL_RS02305 0.58 -4.9 100,229 - SGL_RS02305 0.59 -2.5 100,354 - SGL_RS02305 0.68 -4.8 100,439 + SGL_RS02305 0.74 -3.9 100,467 + SGL_RS02305 0.77 -5.7 100,472 + SGL_RS02305 0.77 -3.5 100,480 - SGL_RS02305 0.77 -2.9 100,676 - -1.6 100,678 + -3.2 100,684 - -0.3 100,691 - -3.2 101,089 + SGL_RS02310 0.30 +1.8 101,130 - SGL_RS02310 0.34 +1.5 101,139 - SGL_RS02310 0.35 +0.7 101,149 - SGL_RS02310 0.36 +1.6 101,179 + SGL_RS02310 0.39 +1.3 101,179 + SGL_RS02310 0.39 +1.5 101,187 - SGL_RS02310 0.40 +1.1 101,212 - SGL_RS02310 0.43 +1.9 101,218 - SGL_RS02310 0.43 +1.2 101,220 + SGL_RS02310 0.44 +1.0 101,308 + SGL_RS02310 0.53 +1.4 101,358 - SGL_RS02310 0.58 +1.3 101,363 - SGL_RS02310 0.58 +1.6 101,363 - SGL_RS02310 0.58 +2.5 101,415 - SGL_RS02310 0.63 +1.2 101,428 + SGL_RS02310 0.65 +1.0 101,436 - SGL_RS02310 0.66 +1.6 101,436 - SGL_RS02310 0.66 +1.5 101,449 + SGL_RS02310 0.67 +1.5 101,505 + SGL_RS02310 0.73 +0.5 101,735 + +1.5 101,781 + +0.3 101,781 + +1.5
Or see this region's nucleotide sequence