Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02195 and SGL_RS02200 are separated by 79 nucleotides SGL_RS02200 and SGL_RS02205 are separated by 86 nucleotides SGL_RS02205 and SGL_RS02210 are separated by 153 nucleotides SGL_RS02210 and SGL_RS02215 overlap by 41 nucleotides SGL_RS02215 and SGL_RS02220 are separated by 21 nucleotides
SGL_RS02195: SGL_RS02195 - nucleoside monophosphate kinase, at 75,955 to 76,500
_RS02195
SGL_RS02200: SGL_RS02200 - 4-hydroxy-tetrahydrodipicolinate reductase, at 76,580 to 77,407
_RS02200
SGL_RS02205: SGL_RS02205 - thioredoxin family protein, at 77,494 to 77,826
_RS02205
SGL_RS02210: SGL_RS02210 - DUF2442 domain-containing protein, at 77,980 to 78,246
_RS02210
SGL_RS02215: SGL_RS02215 - DUF4160 domain-containing protein, at 78,206 to 78,445
_RS02215
SGL_RS02220: SGL_RS02220 - phosphoribosylformylglycinamidine synthase subunit PurL, at 78,467 to 80,800
_RS02220
Position (kb) 77
78 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 76.880 kb on - strand, within SGL_RS02200 at 76.880 kb on - strand, within SGL_RS02200 at 77.149 kb on + strand, within SGL_RS02200 at 77.198 kb on - strand, within SGL_RS02200 at 77.205 kb on - strand, within SGL_RS02200 at 77.440 kb on - strand at 77.505 kb on - strand at 77.523 kb on + strand at 77.624 kb on + strand, within SGL_RS02205 at 77.657 kb on + strand, within SGL_RS02205 at 77.708 kb on - strand, within SGL_RS02205 at 77.905 kb on - strand at 78.242 kb on - strand at 78.580 kb on - strand at 78.585 kb on - strand at 78.609 kb on + strand at 78.806 kb on + strand, within SGL_RS02220 at 78.806 kb on + strand, within SGL_RS02220
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 76,880 - SGL_RS02200 0.36 +1.3 76,880 - SGL_RS02200 0.36 -1.9 77,149 + SGL_RS02200 0.69 +0.9 77,198 - SGL_RS02200 0.75 +0.7 77,205 - SGL_RS02200 0.75 -1.9 77,440 - -0.5 77,505 - -1.3 77,523 + -0.3 77,624 + SGL_RS02205 0.39 +0.8 77,657 + SGL_RS02205 0.49 +0.9 77,708 - SGL_RS02205 0.64 +0.9 77,905 - +0.0 78,242 - +0.1 78,580 - -1.8 78,585 - -0.3 78,609 + -2.9 78,806 + SGL_RS02220 0.15 -3.4 78,806 + SGL_RS02220 0.15 -1.6
Or see this region's nucleotide sequence