Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02135 and SGL_RS02140 are separated by 72 nucleotides SGL_RS02140 and SGL_RS02145 are separated by 78 nucleotides SGL_RS02145 and SGL_RS02150 are separated by 72 nucleotides SGL_RS02150 and SGL_RS02155 are separated by 38 nucleotides
SGL_RS02135: SGL_RS02135 - DinB family protein, at 64,393 to 64,914
_RS02135
SGL_RS02140: SGL_RS02140 - class I SAM-dependent methyltransferase, at 64,987 to 65,748
_RS02140
SGL_RS02145: SGL_RS02145 - metallophosphoesterase, at 65,827 to 67,533
_RS02145
SGL_RS02150: SGL_RS02150 - WecB/TagA/CpsF family glycosyltransferase, at 67,606 to 68,361
_RS02150
SGL_RS02155: SGL_RS02155 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB, at 68,400 to 69,059
_RS02155
Position (kb) 65
66
67
68 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 64.828 kb on - strand, within SGL_RS02135 at 65.031 kb on + strand at 65.044 kb on - strand at 65.333 kb on - strand, within SGL_RS02140 at 65.464 kb on - strand, within SGL_RS02140 at 65.589 kb on + strand, within SGL_RS02140 at 65.651 kb on - strand, within SGL_RS02140 at 65.973 kb on - strand at 66.025 kb on + strand, within SGL_RS02145 at 66.329 kb on + strand, within SGL_RS02145 at 66.337 kb on - strand, within SGL_RS02145 at 66.337 kb on - strand, within SGL_RS02145 at 66.545 kb on - strand, within SGL_RS02145 at 66.592 kb on - strand, within SGL_RS02145 at 66.671 kb on - strand, within SGL_RS02145 at 66.752 kb on - strand, within SGL_RS02145 at 66.757 kb on - strand, within SGL_RS02145 at 66.785 kb on + strand, within SGL_RS02145 at 66.785 kb on + strand, within SGL_RS02145 at 66.829 kb on - strand, within SGL_RS02145 at 66.835 kb on + strand, within SGL_RS02145 at 66.877 kb on - strand, within SGL_RS02145 at 66.972 kb on - strand, within SGL_RS02145 at 66.999 kb on + strand, within SGL_RS02145 at 67.043 kb on - strand, within SGL_RS02145 at 67.087 kb on - strand, within SGL_RS02145 at 67.163 kb on - strand, within SGL_RS02145 at 67.166 kb on + strand, within SGL_RS02145 at 67.200 kb on - strand, within SGL_RS02145 at 67.260 kb on - strand, within SGL_RS02145 at 67.322 kb on + strand, within SGL_RS02145 at 67.330 kb on - strand, within SGL_RS02145 at 67.330 kb on - strand, within SGL_RS02145 at 67.330 kb on - strand, within SGL_RS02145 at 67.399 kb on + strand at 67.399 kb on + strand at 67.406 kb on + strand at 67.463 kb on - strand at 67.657 kb on + strand at 67.660 kb on - strand at 67.786 kb on + strand, within SGL_RS02150 at 67.794 kb on - strand, within SGL_RS02150 at 67.823 kb on + strand, within SGL_RS02150 at 67.838 kb on + strand, within SGL_RS02150 at 67.850 kb on + strand, within SGL_RS02150 at 68.167 kb on + strand, within SGL_RS02150 at 68.528 kb on + strand, within SGL_RS02155
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 64,828 - SGL_RS02135 0.83 -0.1 65,031 + +0.6 65,044 - -1.1 65,333 - SGL_RS02140 0.45 +0.7 65,464 - SGL_RS02140 0.63 -0.9 65,589 + SGL_RS02140 0.79 +0.0 65,651 - SGL_RS02140 0.87 -0.2 65,973 - -3.6 66,025 + SGL_RS02145 0.12 -5.2 66,329 + SGL_RS02145 0.29 -5.2 66,337 - SGL_RS02145 0.30 -4.5 66,337 - SGL_RS02145 0.30 -5.3 66,545 - SGL_RS02145 0.42 -3.2 66,592 - SGL_RS02145 0.45 -5.0 66,671 - SGL_RS02145 0.49 -5.1 66,752 - SGL_RS02145 0.54 -5.0 66,757 - SGL_RS02145 0.54 -2.5 66,785 + SGL_RS02145 0.56 -2.6 66,785 + SGL_RS02145 0.56 -4.8 66,829 - SGL_RS02145 0.59 -3.6 66,835 + SGL_RS02145 0.59 -4.7 66,877 - SGL_RS02145 0.62 -5.5 66,972 - SGL_RS02145 0.67 -3.3 66,999 + SGL_RS02145 0.69 -3.4 67,043 - SGL_RS02145 0.71 -4.7 67,087 - SGL_RS02145 0.74 -3.2 67,163 - SGL_RS02145 0.78 -2.6 67,166 + SGL_RS02145 0.78 -2.1 67,200 - SGL_RS02145 0.80 -1.6 67,260 - SGL_RS02145 0.84 -1.6 67,322 + SGL_RS02145 0.88 -6.4 67,330 - SGL_RS02145 0.88 -5.2 67,330 - SGL_RS02145 0.88 -4.9 67,330 - SGL_RS02145 0.88 -2.9 67,399 + -5.0 67,399 + -2.1 67,406 + -3.2 67,463 - -3.2 67,657 + +1.3 67,660 - +2.0 67,786 + SGL_RS02150 0.24 +0.6 67,794 - SGL_RS02150 0.25 +0.5 67,823 + SGL_RS02150 0.29 +1.2 67,838 + SGL_RS02150 0.31 +1.4 67,850 + SGL_RS02150 0.32 +1.6 68,167 + SGL_RS02150 0.74 +1.3 68,528 + SGL_RS02155 0.19 +0.4
Or see this region's nucleotide sequence