Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00795 and SGL_RS00800 are separated by 367 nucleotides SGL_RS00800 and SGL_RS00805 are separated by 10 nucleotides SGL_RS00805 and SGL_RS00810 are separated by 117 nucleotides SGL_RS00810 and SGL_RS00815 overlap by 10 nucleotides
SGL_RS00795: SGL_RS00795 - DUF3854 domain-containing protein, at 32,647 to 35,523
_RS00795
SGL_RS00800: SGL_RS00800 - putative addiction module antidote protein, at 35,891 to 36,178
_RS00800
SGL_RS00805: SGL_RS00805 - type II toxin-antitoxin system RelE/ParE family toxin, at 36,189 to 36,479
_RS00805
SGL_RS00810: SGL_RS00810 - AbrB/MazE/SpoVT family DNA-binding domain-containing protein, at 36,597 to 36,848
_RS00810
SGL_RS00815: SGL_RS00815 - type II toxin-antitoxin system PemK/MazF family toxin, at 36,839 to 37,201
_RS00815
Position (kb) 35
36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 35.587 kb on - strand at 35.917 kb on + strand at 35.979 kb on + strand, within SGL_RS00800 at 35.979 kb on + strand, within SGL_RS00800 at 36.068 kb on + strand, within SGL_RS00800 at 36.076 kb on - strand, within SGL_RS00800 at 36.186 kb on + strand at 36.273 kb on - strand, within SGL_RS00805 at 36.295 kb on - strand, within SGL_RS00805 at 36.295 kb on - strand, within SGL_RS00805 at 36.338 kb on + strand, within SGL_RS00805 at 36.375 kb on - strand, within SGL_RS00805 at 36.441 kb on - strand, within SGL_RS00805 at 36.441 kb on - strand, within SGL_RS00805 at 36.461 kb on - strand at 36.582 kb on + strand at 36.653 kb on - strand, within SGL_RS00810 at 36.658 kb on - strand, within SGL_RS00810 at 36.801 kb on - strand, within SGL_RS00810 at 36.855 kb on - strand at 36.857 kb on - strand at 36.880 kb on - strand, within SGL_RS00815 at 37.136 kb on + strand, within SGL_RS00815 at 37.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 35,587 - -0.6 35,917 + -0.7 35,979 + SGL_RS00800 0.31 +4.2 35,979 + SGL_RS00800 0.31 +3.8 36,068 + SGL_RS00800 0.61 +4.2 36,076 - SGL_RS00800 0.64 +3.6 36,186 + +4.5 36,273 - SGL_RS00805 0.29 -0.2 36,295 - SGL_RS00805 0.36 -0.5 36,295 - SGL_RS00805 0.36 -0.1 36,338 + SGL_RS00805 0.51 -0.5 36,375 - SGL_RS00805 0.64 -1.0 36,441 - SGL_RS00805 0.87 -0.2 36,441 - SGL_RS00805 0.87 -0.1 36,461 - -1.3 36,582 + -1.1 36,653 - SGL_RS00810 0.22 -0.0 36,658 - SGL_RS00810 0.24 -1.0 36,801 - SGL_RS00810 0.81 -0.3 36,855 - -0.5 36,857 - +0.1 36,880 - SGL_RS00815 0.11 +0.0 37,136 + SGL_RS00815 0.82 -0.0 37,171 - -3.0
Or see this region's nucleotide sequence