Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00435 and SGL_RS00440 are separated by 112 nucleotides SGL_RS00440 and SGL_RS00445 overlap by 4 nucleotides SGL_RS00445 and SGL_RS00450 are separated by 135 nucleotides SGL_RS00450 and SGL_RS00455 overlap by 4 nucleotides
SGL_RS00435: SGL_RS00435 - NAD(P)/FAD-dependent oxidoreductase, at 91,355 to 92,692
_RS00435
SGL_RS00440: SGL_RS00440 - type II toxin-antitoxin system VapC family toxin, at 92,805 to 93,236
_RS00440
SGL_RS00445: SGL_RS00445 - type II toxin-antitoxin system prevent-host-death family antitoxin, at 93,233 to 93,520
_RS00445
SGL_RS00450: SGL_RS00450 - recombinase family protein, at 93,656 to 93,865
_RS00450
SGL_RS00455: SGL_RS00455 - WYL domain-containing transcriptional regulator, at 93,862 to 94,866
_RS00455
Position (kb)
93
94 Strain fitness (log2 ratio)
-1
0
1
2
3 at 92.284 kb on + strand, within SGL_RS00435 at 92.294 kb on + strand, within SGL_RS00435 at 92.544 kb on - strand, within SGL_RS00435 at 92.605 kb on + strand at 92.866 kb on - strand, within SGL_RS00440 at 92.866 kb on - strand, within SGL_RS00440 at 92.894 kb on - strand, within SGL_RS00440 at 93.051 kb on + strand, within SGL_RS00440 at 93.113 kb on + strand, within SGL_RS00440 at 93.146 kb on - strand, within SGL_RS00440 at 93.170 kb on - strand, within SGL_RS00440 at 93.400 kb on + strand, within SGL_RS00445 at 93.400 kb on + strand, within SGL_RS00445 at 93.402 kb on + strand, within SGL_RS00445 at 93.435 kb on - strand, within SGL_RS00445 at 93.810 kb on + strand, within SGL_RS00450 at 93.900 kb on + strand at 93.962 kb on + strand at 93.967 kb on + strand, within SGL_RS00455 at 94.082 kb on - strand, within SGL_RS00455 at 94.366 kb on - strand, within SGL_RS00455 at 94.368 kb on - strand, within SGL_RS00455 at 94.368 kb on - strand, within SGL_RS00455
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 92,284 + SGL_RS00435 0.69 -1.2 92,294 + SGL_RS00435 0.70 -1.1 92,544 - SGL_RS00435 0.89 -0.1 92,605 + -0.6 92,866 - SGL_RS00440 0.14 +0.0 92,866 - SGL_RS00440 0.14 +0.2 92,894 - SGL_RS00440 0.21 -0.4 93,051 + SGL_RS00440 0.57 -0.5 93,113 + SGL_RS00440 0.71 +0.4 93,146 - SGL_RS00440 0.79 -0.2 93,170 - SGL_RS00440 0.84 -0.3 93,400 + SGL_RS00445 0.58 +0.6 93,400 + SGL_RS00445 0.58 -0.6 93,402 + SGL_RS00445 0.59 +2.9 93,435 - SGL_RS00445 0.70 +2.7 93,810 + SGL_RS00450 0.73 +0.2 93,900 + -0.2 93,962 + -0.5 93,967 + SGL_RS00455 0.10 +0.6 94,082 - SGL_RS00455 0.22 +0.5 94,366 - SGL_RS00455 0.50 +0.3 94,368 - SGL_RS00455 0.50 +0.3 94,368 - SGL_RS00455 0.50 +0.1
Or see this region's nucleotide sequence