Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18220 and SGL_RS18225 are separated by 38 nucleotides SGL_RS18225 and SGL_RS18230 are separated by 108 nucleotides SGL_RS18230 and SGL_RS18235 are separated by 113 nucleotides
SGL_RS18220: SGL_RS18220 - hypothetical protein, at 3,538,447 to 3,538,803
_RS18220
SGL_RS18225: SGL_RS18225 - cofactor assembly of complex C subunit B, at 3,538,842 to 3,539,387
_RS18225
SGL_RS18230: SGL_RS18230 - DUF2993 domain-containing protein, at 3,539,496 to 3,540,212
_RS18230
SGL_RS18235: SGL_RS18235 - NAD-dependent epimerase/dehydratase, at 3,540,326 to 3,541,258
_RS18235
Position (kb) 3539
3540
3541 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 3539.037 kb on - strand, within SGL_RS18225 at 3539.070 kb on + strand, within SGL_RS18225 at 3539.124 kb on - strand, within SGL_RS18225 at 3539.139 kb on - strand, within SGL_RS18225 at 3539.214 kb on + strand, within SGL_RS18225 at 3539.247 kb on - strand, within SGL_RS18225 at 3539.632 kb on + strand, within SGL_RS18230 at 3539.647 kb on - strand, within SGL_RS18230 at 3539.997 kb on + strand, within SGL_RS18230 at 3540.041 kb on - strand, within SGL_RS18230 at 3540.447 kb on + strand, within SGL_RS18235 at 3540.752 kb on + strand, within SGL_RS18235
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 3,539,037 - SGL_RS18225 0.36 +0.4 3,539,070 + SGL_RS18225 0.42 -0.9 3,539,124 - SGL_RS18225 0.52 -0.7 3,539,139 - SGL_RS18225 0.54 +0.3 3,539,214 + SGL_RS18225 0.68 -0.2 3,539,247 - SGL_RS18225 0.74 +0.0 3,539,632 + SGL_RS18230 0.19 -4.0 3,539,647 - SGL_RS18230 0.21 -2.5 3,539,997 + SGL_RS18230 0.70 -4.2 3,540,041 - SGL_RS18230 0.76 -3.2 3,540,447 + SGL_RS18235 0.13 -1.6 3,540,752 + SGL_RS18235 0.46 -2.6
Or see this region's nucleotide sequence