Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18110 and SGL_RS18115 are separated by 88 nucleotides SGL_RS18115 and SGL_RS18120 are separated by 65 nucleotides SGL_RS18120 and SGL_RS19505 are separated by 51 nucleotides
SGL_RS18110: SGL_RS18110 - peptide chain release factor 1, at 3,520,597 to 3,521,694
_RS18110
SGL_RS18115: SGL_RS18115 - DUF3727 domain-containing protein, at 3,521,783 to 3,522,367
_RS18115
SGL_RS18120: SGL_RS18120 - 5'/3'-nucleotidase SurE, at 3,522,433 to 3,523,260
_RS18120
SGL_RS19505: SGL_RS19505 - hypothetical protein, at 3,523,312 to 3,523,458
_RS19505
Position (kb)
3521
3522
3523 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3521.915 kb on + strand, within SGL_RS18115 at 3522.247 kb on - strand, within SGL_RS18115 at 3522.318 kb on - strand at 3522.323 kb on - strand at 3522.584 kb on - strand, within SGL_RS18120 at 3522.598 kb on - strand, within SGL_RS18120 at 3522.660 kb on + strand, within SGL_RS18120 at 3522.795 kb on - strand, within SGL_RS18120 at 3522.845 kb on - strand, within SGL_RS18120 at 3522.861 kb on - strand, within SGL_RS18120 at 3522.944 kb on - strand, within SGL_RS18120 at 3522.956 kb on - strand, within SGL_RS18120 at 3522.956 kb on - strand, within SGL_RS18120 at 3523.036 kb on + strand, within SGL_RS18120 at 3523.036 kb on + strand, within SGL_RS18120 at 3523.036 kb on + strand, within SGL_RS18120 at 3523.043 kb on + strand, within SGL_RS18120 at 3523.051 kb on - strand, within SGL_RS18120 at 3523.064 kb on - strand, within SGL_RS18120 at 3523.139 kb on + strand, within SGL_RS18120 at 3523.147 kb on - strand, within SGL_RS18120 at 3523.186 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 3,521,915 + SGL_RS18115 0.23 -6.4 3,522,247 - SGL_RS18115 0.79 -3.5 3,522,318 - -4.2 3,522,323 - -4.3 3,522,584 - SGL_RS18120 0.18 -3.9 3,522,598 - SGL_RS18120 0.20 -3.4 3,522,660 + SGL_RS18120 0.27 -5.1 3,522,795 - SGL_RS18120 0.44 -3.0 3,522,845 - SGL_RS18120 0.50 -3.0 3,522,861 - SGL_RS18120 0.52 -2.9 3,522,944 - SGL_RS18120 0.62 -2.1 3,522,956 - SGL_RS18120 0.63 -4.4 3,522,956 - SGL_RS18120 0.63 -2.3 3,523,036 + SGL_RS18120 0.73 -4.5 3,523,036 + SGL_RS18120 0.73 -3.9 3,523,036 + SGL_RS18120 0.73 -2.5 3,523,043 + SGL_RS18120 0.74 -2.2 3,523,051 - SGL_RS18120 0.75 -2.7 3,523,064 - SGL_RS18120 0.76 -1.1 3,523,139 + SGL_RS18120 0.85 -2.2 3,523,147 - SGL_RS18120 0.86 -2.7 3,523,186 - -3.7
Or see this region's nucleotide sequence