Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17320 and SGL_RS17325 overlap by 4 nucleotides SGL_RS17325 and SGL_RS17330 are separated by 297 nucleotides SGL_RS17330 and SGL_RS17335 are separated by 82 nucleotides SGL_RS17335 and SGL_RS17340 are separated by 81 nucleotides
SGL_RS17320: SGL_RS17320 - hypothetical protein, at 3,341,880 to 3,342,128
_RS17320
SGL_RS17325: SGL_RS17325 - putative toxin-antitoxin system toxin component, PIN family, at 3,342,125 to 3,342,559
_RS17325
SGL_RS17330: SGL_RS17330 - biosynthetic arginine decarboxylase, at 3,342,857 to 3,344,944
_RS17330
SGL_RS17335: SGL_RS17335 - hypothetical protein, at 3,345,027 to 3,345,779
_RS17335
SGL_RS17340: SGL_RS17340 - quinolinate synthase NadA, at 3,345,861 to 3,346,817
_RS17340
Position (kb)
3342
3343
3344
3345 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3342.219 kb on - strand, within SGL_RS17325 at 3342.254 kb on + strand, within SGL_RS17325 at 3342.763 kb on + strand at 3342.840 kb on - strand at 3342.918 kb on + strand at 3342.918 kb on + strand at 3342.974 kb on + strand at 3342.988 kb on - strand at 3342.993 kb on + strand at 3343.003 kb on - strand at 3343.095 kb on + strand, within SGL_RS17330 at 3343.131 kb on - strand, within SGL_RS17330 at 3343.361 kb on - strand, within SGL_RS17330 at 3343.400 kb on - strand, within SGL_RS17330 at 3343.402 kb on - strand, within SGL_RS17330 at 3343.407 kb on - strand, within SGL_RS17330 at 3343.412 kb on - strand, within SGL_RS17330 at 3343.725 kb on + strand, within SGL_RS17330 at 3343.754 kb on + strand, within SGL_RS17330 at 3343.809 kb on + strand, within SGL_RS17330 at 3343.853 kb on + strand, within SGL_RS17330 at 3343.861 kb on - strand, within SGL_RS17330 at 3343.874 kb on - strand, within SGL_RS17330 at 3343.958 kb on + strand, within SGL_RS17330 at 3343.992 kb on + strand, within SGL_RS17330 at 3344.000 kb on - strand, within SGL_RS17330 at 3344.000 kb on - strand, within SGL_RS17330 at 3344.005 kb on - strand, within SGL_RS17330 at 3344.101 kb on + strand, within SGL_RS17330 at 3344.256 kb on + strand, within SGL_RS17330 at 3344.743 kb on + strand at 3344.822 kb on + strand at 3344.858 kb on - strand at 3345.010 kb on - strand at 3345.060 kb on - strand at 3345.078 kb on + strand at 3345.089 kb on + strand at 3345.165 kb on - strand, within SGL_RS17335 at 3345.203 kb on + strand, within SGL_RS17335 at 3345.279 kb on + strand, within SGL_RS17335 at 3345.336 kb on + strand, within SGL_RS17335 at 3345.431 kb on + strand, within SGL_RS17335 at 3345.505 kb on + strand, within SGL_RS17335 at 3345.546 kb on - strand, within SGL_RS17335 at 3345.683 kb on + strand, within SGL_RS17335 at 3345.724 kb on + strand at 3345.757 kb on + strand at 3345.814 kb on + strand at 3345.867 kb on - strand at 3345.867 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 3,342,219 - SGL_RS17325 0.22 -0.5 3,342,254 + SGL_RS17325 0.30 +0.7 3,342,763 + -0.9 3,342,840 - +0.6 3,342,918 + -5.0 3,342,918 + -5.4 3,342,974 + +0.2 3,342,988 - -5.1 3,342,993 + -6.4 3,343,003 - -3.5 3,343,095 + SGL_RS17330 0.11 -5.2 3,343,131 - SGL_RS17330 0.13 -4.3 3,343,361 - SGL_RS17330 0.24 -5.2 3,343,400 - SGL_RS17330 0.26 -2.6 3,343,402 - SGL_RS17330 0.26 -4.6 3,343,407 - SGL_RS17330 0.26 -6.5 3,343,412 - SGL_RS17330 0.27 -3.6 3,343,725 + SGL_RS17330 0.42 -5.1 3,343,754 + SGL_RS17330 0.43 -5.0 3,343,809 + SGL_RS17330 0.46 -5.2 3,343,853 + SGL_RS17330 0.48 -4.0 3,343,861 - SGL_RS17330 0.48 -4.4 3,343,874 - SGL_RS17330 0.49 -2.2 3,343,958 + SGL_RS17330 0.53 -4.5 3,343,992 + SGL_RS17330 0.54 -4.8 3,344,000 - SGL_RS17330 0.55 -4.7 3,344,000 - SGL_RS17330 0.55 -4.3 3,344,005 - SGL_RS17330 0.55 -5.6 3,344,101 + SGL_RS17330 0.60 -4.9 3,344,256 + SGL_RS17330 0.67 -5.1 3,344,743 + -4.8 3,344,822 + -5.0 3,344,858 - -5.4 3,345,010 - +0.6 3,345,060 - -2.8 3,345,078 + -2.3 3,345,089 + -1.9 3,345,165 - SGL_RS17335 0.18 -3.9 3,345,203 + SGL_RS17335 0.23 -1.1 3,345,279 + SGL_RS17335 0.33 -2.6 3,345,336 + SGL_RS17335 0.41 -1.7 3,345,431 + SGL_RS17335 0.54 -1.8 3,345,505 + SGL_RS17335 0.63 -1.8 3,345,546 - SGL_RS17335 0.69 -2.3 3,345,683 + SGL_RS17335 0.87 -1.4 3,345,724 + -2.4 3,345,757 + -1.5 3,345,814 + -1.7 3,345,867 - -0.7 3,345,867 - +0.5
Or see this region's nucleotide sequence