Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16065 and SGL_RS16070 are separated by 79 nucleotides SGL_RS16070 and SGL_RS16075 are separated by 114 nucleotides
SGL_RS16065: SGL_RS16065 - CusA/CzcA family heavy metal efflux RND transporter, at 3,051,772 to 3,054,936
_RS16065
SGL_RS16070: SGL_RS16070 - glycoside hydrolase family protein, at 3,055,016 to 3,055,699
_RS16070
SGL_RS16075: SGL_RS16075 - MFS transporter, at 3,055,814 to 3,057,151
_RS16075
Position (kb)
3055
3056 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 3054.090 kb on + strand, within SGL_RS16065 at 3054.201 kb on + strand, within SGL_RS16065 at 3054.420 kb on - strand, within SGL_RS16065 at 3054.473 kb on + strand, within SGL_RS16065 at 3054.548 kb on + strand, within SGL_RS16065 at 3054.558 kb on + strand, within SGL_RS16065 at 3054.579 kb on - strand, within SGL_RS16065 at 3054.581 kb on + strand, within SGL_RS16065 at 3054.589 kb on - strand, within SGL_RS16065 at 3054.661 kb on - strand at 3054.666 kb on + strand at 3054.700 kb on + strand at 3054.708 kb on - strand at 3054.822 kb on - strand at 3054.869 kb on + strand at 3055.512 kb on + strand, within SGL_RS16070 at 3055.553 kb on + strand, within SGL_RS16070 at 3055.556 kb on - strand, within SGL_RS16070 at 3055.865 kb on + strand at 3055.867 kb on + strand at 3055.867 kb on + strand at 3055.875 kb on - strand at 3055.875 kb on - strand at 3055.875 kb on - strand at 3055.904 kb on + strand at 3055.904 kb on + strand at 3055.912 kb on - strand at 3055.912 kb on - strand at 3055.912 kb on - strand at 3055.914 kb on + strand at 3055.915 kb on + strand at 3055.917 kb on - strand at 3055.961 kb on + strand, within SGL_RS16075 at 3055.969 kb on - strand, within SGL_RS16075 at 3055.980 kb on - strand, within SGL_RS16075 at 3055.987 kb on - strand, within SGL_RS16075 at 3055.994 kb on - strand, within SGL_RS16075 at 3056.006 kb on + strand, within SGL_RS16075 at 3056.026 kb on - strand, within SGL_RS16075 at 3056.029 kb on + strand, within SGL_RS16075 at 3056.078 kb on - strand, within SGL_RS16075 at 3056.078 kb on - strand, within SGL_RS16075 at 3056.094 kb on - strand, within SGL_RS16075 at 3056.116 kb on + strand, within SGL_RS16075 at 3056.117 kb on + strand, within SGL_RS16075 at 3056.182 kb on + strand, within SGL_RS16075 at 3056.195 kb on + strand, within SGL_RS16075 at 3056.240 kb on + strand, within SGL_RS16075 at 3056.240 kb on + strand, within SGL_RS16075 at 3056.248 kb on - strand, within SGL_RS16075 at 3056.248 kb on - strand, within SGL_RS16075 at 3056.294 kb on - strand, within SGL_RS16075 at 3056.298 kb on - strand, within SGL_RS16075 at 3056.418 kb on - strand, within SGL_RS16075 at 3056.428 kb on - strand, within SGL_RS16075 at 3056.529 kb on - strand, within SGL_RS16075 at 3056.582 kb on - strand, within SGL_RS16075 at 3056.653 kb on + strand, within SGL_RS16075
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 3,054,090 + SGL_RS16065 0.73 -6.1 3,054,201 + SGL_RS16065 0.77 -4.6 3,054,420 - SGL_RS16065 0.84 -6.6 3,054,473 + SGL_RS16065 0.85 -6.5 3,054,548 + SGL_RS16065 0.88 -4.4 3,054,558 + SGL_RS16065 0.88 -7.1 3,054,579 - SGL_RS16065 0.89 -3.1 3,054,581 + SGL_RS16065 0.89 -5.7 3,054,589 - SGL_RS16065 0.89 -7.4 3,054,661 - -8.0 3,054,666 + -2.6 3,054,700 + -5.3 3,054,708 - -7.1 3,054,822 - -7.8 3,054,869 + -0.7 3,055,512 + SGL_RS16070 0.73 -1.7 3,055,553 + SGL_RS16070 0.79 -2.0 3,055,556 - SGL_RS16070 0.79 -1.2 3,055,865 + -6.4 3,055,867 + -4.3 3,055,867 + -5.1 3,055,875 - -4.3 3,055,875 - -5.3 3,055,875 - -7.2 3,055,904 + -5.0 3,055,904 + -4.4 3,055,912 - -6.3 3,055,912 - -4.0 3,055,912 - -5.5 3,055,914 + -7.7 3,055,915 + -7.3 3,055,917 - -7.4 3,055,961 + SGL_RS16075 0.11 -7.2 3,055,969 - SGL_RS16075 0.12 -5.4 3,055,980 - SGL_RS16075 0.12 -6.3 3,055,987 - SGL_RS16075 0.13 -5.6 3,055,994 - SGL_RS16075 0.13 -6.8 3,056,006 + SGL_RS16075 0.14 -6.1 3,056,026 - SGL_RS16075 0.16 -6.5 3,056,029 + SGL_RS16075 0.16 -6.6 3,056,078 - SGL_RS16075 0.20 -6.2 3,056,078 - SGL_RS16075 0.20 -5.4 3,056,094 - SGL_RS16075 0.21 -5.5 3,056,116 + SGL_RS16075 0.23 -3.9 3,056,117 + SGL_RS16075 0.23 -7.2 3,056,182 + SGL_RS16075 0.28 -7.1 3,056,195 + SGL_RS16075 0.28 -3.9 3,056,240 + SGL_RS16075 0.32 -6.8 3,056,240 + SGL_RS16075 0.32 -6.7 3,056,248 - SGL_RS16075 0.32 -8.5 3,056,248 - SGL_RS16075 0.32 -4.4 3,056,294 - SGL_RS16075 0.36 -6.9 3,056,298 - SGL_RS16075 0.36 -5.3 3,056,418 - SGL_RS16075 0.45 -6.8 3,056,428 - SGL_RS16075 0.46 -6.3 3,056,529 - SGL_RS16075 0.53 -5.8 3,056,582 - SGL_RS16075 0.57 -7.0 3,056,653 + SGL_RS16075 0.63 -5.3
Or see this region's nucleotide sequence