Experiment: Nickel (II) sulfate 3 uM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt SGL_RS15825 and SGL_RS15830 are separated by 225 nucleotides SGL_RS15830 and SGL_RS15835 are separated by 332 nucleotides 
        SGL_RS15825: SGL_RS15825 - phosphomethylpyrimidine synthase, at 2,999,181 to 3,000,560 
        _RS15825 
         
        
        SGL_RS15830: SGL_RS15830 - YihY/virulence factor BrkB family protein, at 3,000,786 to 3,001,742 
        _RS15830 
         
        
        SGL_RS15835: SGL_RS15835 - TrmJ/YjtD family RNA methyltransferase, at 3,002,075 to 3,002,797 
        _RS15835 
         Position (kb) 3000 
3001 
3002 Strain fitness (log2 ratio) -4 
-3 
-2 
-1 
0 
1 at 3000.561 kb on + strand at 3000.669 kb on - strand at 3000.733 kb on + strand at 3000.744 kb on + strand at 3000.789 kb on - strand at 3001.930 kb on + strand at 3001.930 kb on + strand at 3002.186 kb on + strand, within SGL_RS15835 at 3002.227 kb on + strand, within SGL_RS15835 at 3002.289 kb on + strand, within SGL_RS15835 at 3002.297 kb on - strand, within SGL_RS15835 at 3002.370 kb on - strand, within SGL_RS15835 at 3002.407 kb on - strand, within SGL_RS15835 at 3002.407 kb on - strand, within SGL_RS15835 at 3002.422 kb on - strand, within SGL_RS15835 at 3002.445 kb on + strand, within SGL_RS15835 at 3002.451 kb on - strand, within SGL_RS15835 at 3002.462 kb on - strand, within SGL_RS15835 at 3002.499 kb on + strand, within SGL_RS15835 at 3002.571 kb on - strand, within SGL_RS15835 at 3002.587 kb on - strand, within SGL_RS15835 at 3002.613 kb on - strand, within SGL_RS15835 at 3002.619 kb on - strand, within SGL_RS15835 at 3002.630 kb on + strand, within SGL_RS15835 at 3002.735 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nickel (II) sulfate 3 uM remove 3,000,561 +  +1.3 3,000,669 -  -0.1 3,000,733 +  +0.2 3,000,744 +  +0.1 3,000,789 -  -1.1 3,001,930 +  +0.2 3,001,930 +  -0.4 3,002,186 +  SGL_RS15835 0.15  -2.5 3,002,227 +  SGL_RS15835 0.21  -3.0 3,002,289 +  SGL_RS15835 0.30  -3.0 3,002,297 -  SGL_RS15835 0.31  -3.4 3,002,370 -  SGL_RS15835 0.41  -2.5 3,002,407 -  SGL_RS15835 0.46  -2.9 3,002,407 -  SGL_RS15835 0.46  -3.6 3,002,422 -  SGL_RS15835 0.48  -3.0 3,002,445 +  SGL_RS15835 0.51  -3.3 3,002,451 -  SGL_RS15835 0.52  -2.7 3,002,462 -  SGL_RS15835 0.54  -2.9 3,002,499 +  SGL_RS15835 0.59  -3.4 3,002,571 -  SGL_RS15835 0.69  -2.9 3,002,587 -  SGL_RS15835 0.71  -3.0 3,002,613 -  SGL_RS15835 0.74  -3.9 3,002,619 -  SGL_RS15835 0.75  -4.7 3,002,630 +  SGL_RS15835 0.77  -3.0 3,002,735 -  -1.7 
Or see this region's nucleotide sequence