Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15645 and SGL_RS15650 are separated by 363 nucleotides SGL_RS15650 and SGL_RS15655 are separated by 175 nucleotides SGL_RS15655 and SGL_RS15660 are separated by 58 nucleotides SGL_RS15660 and SGL_RS15665 are separated by 169 nucleotides
SGL_RS15645: SGL_RS15645 - NAD(P)/FAD-dependent oxidoreductase, at 2,959,530 to 2,960,756
_RS15645
SGL_RS15650: SGL_RS15650 - thioredoxin, at 2,961,120 to 2,961,443
_RS15650
SGL_RS15655: SGL_RS15655 - asparaginase, at 2,961,619 to 2,962,647
_RS15655
SGL_RS15660: SGL_RS15660 - CGLD27 family protein, at 2,962,706 to 2,963,227
_RS15660
SGL_RS15665: SGL_RS15665 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 2,963,397 to 2,964,512
_RS15665
Position (kb)
2961
2962
2963 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 2960.630 kb on + strand, within SGL_RS15645 at 2960.768 kb on + strand at 2960.768 kb on + strand at 2960.768 kb on + strand at 2960.884 kb on - strand at 2961.376 kb on - strand, within SGL_RS15650 at 2961.481 kb on + strand at 2961.617 kb on + strand at 2961.735 kb on + strand, within SGL_RS15655 at 2961.742 kb on + strand, within SGL_RS15655 at 2961.793 kb on + strand, within SGL_RS15655 at 2961.801 kb on - strand, within SGL_RS15655 at 2961.801 kb on - strand, within SGL_RS15655 at 2961.801 kb on - strand, within SGL_RS15655 at 2961.801 kb on - strand, within SGL_RS15655 at 2961.811 kb on - strand, within SGL_RS15655 at 2961.926 kb on + strand, within SGL_RS15655 at 2961.934 kb on - strand, within SGL_RS15655 at 2962.013 kb on + strand, within SGL_RS15655 at 2962.053 kb on + strand, within SGL_RS15655 at 2962.112 kb on + strand, within SGL_RS15655 at 2962.152 kb on + strand, within SGL_RS15655 at 2962.311 kb on + strand, within SGL_RS15655 at 2962.316 kb on + strand, within SGL_RS15655 at 2962.338 kb on - strand, within SGL_RS15655 at 2962.417 kb on + strand, within SGL_RS15655 at 2962.449 kb on + strand, within SGL_RS15655 at 2962.495 kb on + strand, within SGL_RS15655 at 2962.549 kb on - strand at 2962.707 kb on - strand at 2962.768 kb on - strand, within SGL_RS15660 at 2962.921 kb on + strand, within SGL_RS15660 at 2962.934 kb on - strand, within SGL_RS15660 at 2963.075 kb on + strand, within SGL_RS15660 at 2963.075 kb on + strand, within SGL_RS15660 at 2963.082 kb on - strand, within SGL_RS15660 at 2963.089 kb on + strand, within SGL_RS15660 at 2963.118 kb on - strand, within SGL_RS15660 at 2963.190 kb on + strand at 2963.198 kb on - strand at 2963.203 kb on - strand at 2963.257 kb on + strand at 2963.303 kb on - strand at 2963.369 kb on + strand at 2963.393 kb on + strand at 2963.401 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,960,630 + SGL_RS15645 0.90 +0.3 2,960,768 + +1.0 2,960,768 + -0.9 2,960,768 + +0.3 2,960,884 - +0.1 2,961,376 - SGL_RS15650 0.79 -2.8 2,961,481 + +0.0 2,961,617 + +0.1 2,961,735 + SGL_RS15655 0.11 -6.4 2,961,742 + SGL_RS15655 0.12 -4.9 2,961,793 + SGL_RS15655 0.17 -3.8 2,961,801 - SGL_RS15655 0.18 -4.1 2,961,801 - SGL_RS15655 0.18 -2.4 2,961,801 - SGL_RS15655 0.18 -4.1 2,961,801 - SGL_RS15655 0.18 -4.9 2,961,811 - SGL_RS15655 0.19 -6.0 2,961,926 + SGL_RS15655 0.30 -4.5 2,961,934 - SGL_RS15655 0.31 -7.6 2,962,013 + SGL_RS15655 0.38 -6.2 2,962,053 + SGL_RS15655 0.42 -4.1 2,962,112 + SGL_RS15655 0.48 -6.1 2,962,152 + SGL_RS15655 0.52 -6.0 2,962,311 + SGL_RS15655 0.67 -4.2 2,962,316 + SGL_RS15655 0.68 -5.6 2,962,338 - SGL_RS15655 0.70 -5.3 2,962,417 + SGL_RS15655 0.78 -6.0 2,962,449 + SGL_RS15655 0.81 -6.5 2,962,495 + SGL_RS15655 0.85 -4.0 2,962,549 - -1.0 2,962,707 - +0.1 2,962,768 - SGL_RS15660 0.12 +0.4 2,962,921 + SGL_RS15660 0.41 +0.4 2,962,934 - SGL_RS15660 0.44 +0.0 2,963,075 + SGL_RS15660 0.71 +0.5 2,963,075 + SGL_RS15660 0.71 -0.3 2,963,082 - SGL_RS15660 0.72 -0.1 2,963,089 + SGL_RS15660 0.73 +0.0 2,963,118 - SGL_RS15660 0.79 +0.1 2,963,190 + +0.2 2,963,198 - +0.7 2,963,203 - +0.5 2,963,257 + +1.0 2,963,303 - +1.3 2,963,369 + -1.3 2,963,393 + +1.4 2,963,401 - -1.4
Or see this region's nucleotide sequence