Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15040 and SGL_RS15045 are separated by 110 nucleotides SGL_RS15045 and SGL_RS15050 are separated by 129 nucleotides SGL_RS15050 and SGL_RS15055 overlap by 25 nucleotides SGL_RS15055 and SGL_RS15060 are separated by 6 nucleotides
SGL_RS15040: SGL_RS15040 - acyl-CoA desaturase, at 2,822,579 to 2,823,535
_RS15040
SGL_RS15045: SGL_RS15045 - aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme, at 2,823,646 to 2,824,893
_RS15045
SGL_RS15050: SGL_RS15050 - MliC family protein, at 2,825,023 to 2,825,355
_RS15050
SGL_RS15055: SGL_RS15055 - hypothetical protein, at 2,825,331 to 2,825,711
_RS15055
SGL_RS15060: SGL_RS15060 - PstS family phosphate ABC transporter substrate-binding protein, at 2,825,718 to 2,826,641
_RS15060
Position (kb)
2823
2824
2825 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2823.780 kb on - strand, within SGL_RS15045 at 2823.789 kb on + strand, within SGL_RS15045 at 2823.797 kb on + strand, within SGL_RS15045 at 2823.857 kb on - strand, within SGL_RS15045 at 2823.857 kb on - strand, within SGL_RS15045 at 2823.904 kb on + strand, within SGL_RS15045 at 2823.916 kb on + strand, within SGL_RS15045 at 2823.970 kb on + strand, within SGL_RS15045 at 2823.970 kb on + strand, within SGL_RS15045 at 2823.970 kb on + strand, within SGL_RS15045 at 2823.978 kb on - strand, within SGL_RS15045 at 2823.978 kb on - strand, within SGL_RS15045 at 2824.046 kb on - strand, within SGL_RS15045 at 2824.090 kb on + strand, within SGL_RS15045 at 2824.165 kb on + strand, within SGL_RS15045 at 2824.358 kb on - strand, within SGL_RS15045 at 2824.384 kb on - strand, within SGL_RS15045 at 2824.513 kb on - strand, within SGL_RS15045 at 2824.649 kb on - strand, within SGL_RS15045 at 2824.724 kb on - strand, within SGL_RS15045 at 2824.731 kb on - strand, within SGL_RS15045 at 2824.785 kb on + strand at 2824.824 kb on - strand at 2824.925 kb on - strand at 2824.935 kb on + strand at 2825.224 kb on + strand, within SGL_RS15050 at 2825.231 kb on - strand, within SGL_RS15050 at 2825.287 kb on - strand, within SGL_RS15050 at 2825.495 kb on + strand, within SGL_RS15055 at 2825.522 kb on + strand, within SGL_RS15055 at 2825.537 kb on - strand, within SGL_RS15055 at 2825.728 kb on + strand at 2825.798 kb on - strand at 2825.863 kb on - strand, within SGL_RS15060
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,823,780 - SGL_RS15045 0.11 -2.1 2,823,789 + SGL_RS15045 0.11 -3.0 2,823,797 + SGL_RS15045 0.12 -4.7 2,823,857 - SGL_RS15045 0.17 -4.0 2,823,857 - SGL_RS15045 0.17 -4.6 2,823,904 + SGL_RS15045 0.21 -3.2 2,823,916 + SGL_RS15045 0.22 -5.9 2,823,970 + SGL_RS15045 0.26 -5.3 2,823,970 + SGL_RS15045 0.26 -2.3 2,823,970 + SGL_RS15045 0.26 -1.5 2,823,978 - SGL_RS15045 0.27 -3.5 2,823,978 - SGL_RS15045 0.27 -3.2 2,824,046 - SGL_RS15045 0.32 -4.2 2,824,090 + SGL_RS15045 0.36 -4.7 2,824,165 + SGL_RS15045 0.42 -2.1 2,824,358 - SGL_RS15045 0.57 -1.5 2,824,384 - SGL_RS15045 0.59 -3.1 2,824,513 - SGL_RS15045 0.69 -5.1 2,824,649 - SGL_RS15045 0.80 -4.7 2,824,724 - SGL_RS15045 0.86 -5.5 2,824,731 - SGL_RS15045 0.87 -1.9 2,824,785 + -2.6 2,824,824 - -3.9 2,824,925 - -0.1 2,824,935 + -1.0 2,825,224 + SGL_RS15050 0.60 +0.3 2,825,231 - SGL_RS15050 0.62 +0.2 2,825,287 - SGL_RS15050 0.79 -0.9 2,825,495 + SGL_RS15055 0.43 +0.1 2,825,522 + SGL_RS15055 0.50 +0.2 2,825,537 - SGL_RS15055 0.54 +0.5 2,825,728 + +0.3 2,825,798 - +1.5 2,825,863 - SGL_RS15060 0.16 +0.4
Or see this region's nucleotide sequence