Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14340 and SGL_RS14345 are separated by 130 nucleotides SGL_RS14345 and SGL_RS14350 are separated by 14 nucleotides SGL_RS14350 and SGL_RS14355 are separated by 10 nucleotides SGL_RS14355 and SGL_RS14360 are separated by 511 nucleotides
SGL_RS14340: SGL_RS14340 - protein phosphatase 2C domain-containing protein, at 2,671,895 to 2,673,970
_RS14340
SGL_RS14345: SGL_RS14345 - VWA domain-containing protein, at 2,674,101 to 2,674,721
_RS14345
SGL_RS14350: SGL_RS14350 - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase, at 2,674,736 to 2,676,196
_RS14350
SGL_RS14355: SGL_RS14355 - hypothetical protein, at 2,676,207 to 2,676,425
_RS14355
SGL_RS14360: SGL_RS14360 - carbon-nitrogen hydrolase family protein, at 2,676,937 to 2,677,755
_RS14360
Position (kb)
2674
2675
2676
2677 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2673.753 kb on - strand, within SGL_RS14340 at 2673.788 kb on + strand at 2673.881 kb on + strand at 2673.961 kb on - strand at 2673.967 kb on + strand at 2673.980 kb on - strand at 2673.980 kb on - strand at 2674.103 kb on - strand at 2674.215 kb on + strand, within SGL_RS14345 at 2674.334 kb on + strand, within SGL_RS14345 at 2674.368 kb on + strand, within SGL_RS14345 at 2674.368 kb on + strand, within SGL_RS14345 at 2674.376 kb on - strand, within SGL_RS14345 at 2674.724 kb on - strand at 2675.145 kb on + strand, within SGL_RS14350 at 2675.181 kb on - strand, within SGL_RS14350 at 2675.391 kb on + strand, within SGL_RS14350 at 2675.393 kb on + strand, within SGL_RS14350 at 2675.411 kb on - strand, within SGL_RS14350 at 2675.411 kb on - strand, within SGL_RS14350 at 2675.429 kb on + strand, within SGL_RS14350 at 2675.694 kb on - strand, within SGL_RS14350 at 2675.717 kb on - strand, within SGL_RS14350 at 2675.724 kb on - strand, within SGL_RS14350 at 2675.798 kb on - strand, within SGL_RS14350 at 2675.798 kb on - strand, within SGL_RS14350 at 2675.869 kb on - strand, within SGL_RS14350 at 2675.935 kb on - strand, within SGL_RS14350 at 2676.009 kb on + strand, within SGL_RS14350 at 2676.134 kb on - strand at 2676.153 kb on - strand at 2676.153 kb on - strand at 2676.213 kb on - strand at 2676.670 kb on - strand at 2676.894 kb on - strand at 2676.900 kb on + strand at 2676.900 kb on + strand at 2676.908 kb on - strand at 2676.910 kb on + strand at 2676.992 kb on - strand at 2677.055 kb on - strand, within SGL_RS14360 at 2677.088 kb on - strand, within SGL_RS14360 at 2677.183 kb on + strand, within SGL_RS14360
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,673,753 - SGL_RS14340 0.89 +1.6 2,673,788 + +0.6 2,673,881 + +2.6 2,673,961 - +2.8 2,673,967 + +2.3 2,673,980 - +1.1 2,673,980 - +1.5 2,674,103 - +0.1 2,674,215 + SGL_RS14345 0.18 +0.8 2,674,334 + SGL_RS14345 0.38 -0.4 2,674,368 + SGL_RS14345 0.43 -0.5 2,674,368 + SGL_RS14345 0.43 -0.1 2,674,376 - SGL_RS14345 0.44 -0.5 2,674,724 - -1.7 2,675,145 + SGL_RS14350 0.28 -1.5 2,675,181 - SGL_RS14350 0.30 -1.1 2,675,391 + SGL_RS14350 0.45 -1.8 2,675,393 + SGL_RS14350 0.45 -0.8 2,675,411 - SGL_RS14350 0.46 -1.5 2,675,411 - SGL_RS14350 0.46 -1.8 2,675,429 + SGL_RS14350 0.47 -1.5 2,675,694 - SGL_RS14350 0.66 -1.3 2,675,717 - SGL_RS14350 0.67 -1.9 2,675,724 - SGL_RS14350 0.68 -1.5 2,675,798 - SGL_RS14350 0.73 -1.3 2,675,798 - SGL_RS14350 0.73 -0.4 2,675,869 - SGL_RS14350 0.78 -1.1 2,675,935 - SGL_RS14350 0.82 -1.1 2,676,009 + SGL_RS14350 0.87 -2.4 2,676,134 - -1.5 2,676,153 - -0.8 2,676,153 - -1.4 2,676,213 - +0.0 2,676,670 - +0.1 2,676,894 - +0.1 2,676,900 + -0.5 2,676,900 + +0.1 2,676,908 - +0.0 2,676,910 + -0.8 2,676,992 - +3.7 2,677,055 - SGL_RS14360 0.14 +4.2 2,677,088 - SGL_RS14360 0.18 +3.4 2,677,183 + SGL_RS14360 0.30 +3.4
Or see this region's nucleotide sequence